Cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA for genome editing

Methods in Molecular Biology

Volumn 1507, Issue , 2017, Pages 81-94

  Suresh, Bharathi ^a,b   Ramakrishna, Suresh ^c   Kim, Hyongbum ^a,b  

^a Yonsei University College of Medicine   (South Korea)

^b Yonsei University   (South Korea)

^c Hanyang University   (South Korea)

Author keywords

Cas9 conjugation; Cas9 protein purification; Dialysis; In vitro sgRNA synthesis; Protein delivery; T7E1 assay

Indexed keywords

ARGININE; CASPASE 9; CELL PENETRATING PEPTIDE; CYSTEINE; GENOMIC DNA; GLYCINE; GUIDE RNA; LEUCINE; POLYHISTIDINE TAG; RECOMBINANT PROTEIN; VIRUS VECTOR; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; ENDONUCLEASE;

AMINO TERMINAL SEQUENCE; CELL MEMBRANE; CELL MUTANT; CYTOTOXICITY; DNA SEQUENCE; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE DISRUPTION; GENE MUTATION; HUMAN; HUMAN CELL; IMMUNE RESPONSE; MOLECULAR CLONING; NUCLEAR LOCALIZATION SIGNAL; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN EXPRESSION; RNA EDITING; RNA SYNTHESIS; BACTERIUM TRANSFORMATION; CHEMISTRY; CRISPR CAS SYSTEM; ESCHERICHIA COLI; GENE EDITING; GENETICS; HEK293 CELL LINE; METABOLISM; PROCEDURES;

BACTERIAL PROTEINS; CELL-PENETRATING PEPTIDES; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; ENDONUCLEASES; ESCHERICHIA COLI; GENE EDITING; HEK293 CELLS; HUMANS; PLASMIDS; RNA, GUIDE; TRANSFORMATION, BACTERIAL;

EID: 84994853550     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6518-2_7     Document Type: Chapter

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