CRISPR/Cas-mediated knockin in human pluripotent stem cells

Methods in Molecular Biology

Volumn 1513, Issue , 2017, Pages 119-140

  Verma, Nipun ^a,b   Zhu, Zengrong ^a   Huangfu, Danwei ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

CRISPR Cas; Epitope tag; Fluorescent reporter; Gene targeting; Homologous recombination; Human pluripotent stem cells (hPSCs); Knockin

Indexed keywords

GUIDE RNA; NUCLEASE; SINGLE STRANDED DNA; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; DOXYCYCLINE; ENDONUCLEASE; EPITOPE; FLUORESCENT PROTEIN 583; GREEN FLUORESCENT PROTEIN; LIPID; LIPOFECTAMINE; PHOTOPROTEIN;

ANIMAL CELL; CELL SCREENING; CLONAL CELL LINE; CRISPR CAS SYSTEM; GENE CONSTRUCT; GENE OVEREXPRESSION; GENE TARGETING; GENETIC ENGINEERING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; REPORTER GENE; SEQUENCE ANALYSIS; STEM CELL EXPANSION; VALIDATION STUDY; WESTERN BLOTTING; CHEMISTRY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOLOGY; DRUG EFFECTS; GENE KNOCK-IN; GENE LOCUS; GENE VECTOR; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; PROCEDURES;

BACTERIAL PROTEINS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DOXYCYCLINE; ENDONUCLEASES; EPITOPES; GENE KNOCK-IN TECHNIQUES; GENES, REPORTER; GENETIC LOCI; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HOMOLOGOUS RECOMBINATION; HUMANS; LIPIDS; LUMINESCENT PROTEINS; PLURIPOTENT STEM CELLS; RNA, GUIDE; TRANSFECTION;

EID: 84994311202     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6539-7_9     Document Type: Chapter

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