Flash-and-freeze: Coordinating optogenetic stimulation with rapid freezing to visualize membrane dynamics at synapses with millisecond resolution

Frontiers in Synaptic Neuroscience

Volumn 8, Issue AUG, 2016, Pages

  Watanabe, Shigeki ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Electron microscopy; Flash and freeze; High pressure freezing; Optogenetics; Synaptic cell biology; Synaptic transmission; Synaptic vesicle exocytosis; Synaptic vesicles

Indexed keywords

CHANNELRHODOPSIN; RHODOPSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLUE LIGHT; BRAIN NERVE CELL; CAENORHABDITIS ELEGANS; CELL MEMBRANE TRANSPORT; CELL STRUCTURE; CONTROLLED STUDY; ENDOCYTOSIS; EXOCYTOSIS; FLASH AND FREEZE APPROACH; HETEROLOGOUS EXPRESSION; HIPPOCAMPUS; IMAGE ANALYSIS; MEMBRANE BIOLOGY; MICROMORPHOLOGY; MOTONEURON; MOUSE; NERVE POTENTIAL; NEUROLOGICAL AND SENSORIAL PROCEDURES; NEWBORN; NONHUMAN; OPTICAL RESOLUTION; OPTOGENETICS; PHOTOSTIMULATION; SYNAPSE; SYNAPTIC TRANSMISSION; ULTRAFAST ENDOCYTOSIS;

EID: 84993966769     PISSN: None     EISSN: 16633563     Source Type: Journal    
DOI: 10.3389/fnsyn.2016.00024     Document Type: Article

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