The convenience of single homology arm donor DNA and CRISPR/Cas9-nickase for targeted insertion of long DNA fragment

Cell Journal

Volumn 18, Issue 4, 2016, Pages 532-539

  Basiri, Mohsen ^a,b   Behmanesh, Mehrdad ^a   Tahamtani, Yaser ^b   Khalooghi, Keynoosh ^b   Moradmand, Azadeh ^b   Baharvand, Hossein ^b,c  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b ROYAN INSTITUTE FOR REPRODUCTIVE BIOMEDICINE   (Iran)

^c UNIVERSITY OF SCIENCE AND CULTURE   (Iran)

Author keywords

CRISPR cas systems; Embryonic stem cells; Gene targeting; Pdx1

Indexed keywords

DNA FRAGMENT; RNA; SINGLE GUIDE RNA; TRANSCRIPTION FACTOR PDX 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BASE PAIRING; CONTROLLED STUDY; CRISPR CAS SYSTEM; EMBRYO; EMBRYONIC STEM CELL; EXPERIMENTAL STUDY; GENE INSERTION; GENE LOCUS; GENE TARGETING; INSULINOMA CELL LINE; MOUSE; MUTANT; NONHUMAN; PDX1 GENE; WILD TYPE;

EID: 84992323085     PISSN: 22285806     EISSN: 22285814     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, et al. RNA-guided human genome engineering via Cas9. Science. 2013; 339(6121): 823-626.
2. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013; 339(6121): 819-823.
3. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 2013; 31(9): 827-832.
4. van Gent DC, van der Burg M. Non-homologous end-joining, a sticky affair. Oncogene. 2007; 26(56): 7731-7740.
5. Wyman C, Kanaar R. DNA double-strand break repair: all's well that ends well. Annu Rev Genet. 2006; 40: 363-383.
6. Liang F, Han M, Romanienko PJ, Jasin M. Homology-directed repair is a major double-strand break repair pathway in mammalian cells. Proc Natl Acad Sci USA. 1998; 95(9): 5172-5177.
7. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, et al. Double nicking by rna-guided crispr cas9 for enhanced genome editing specificity. Cell. 2013; 154(6): 1380-1389.
8. Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, et al. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013; 31(9): 833-838.
9. Fujii W, Onuma A, Sugiura K, Naito K. Efficient generation of genome-modified mice via offset-nicking by CRISPR/ Cas system. Biochem Biophys Res Commun. 445(4): 791-794.
10. Rong Z, Zhu S, Xu Y, Fu X. Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template. Protein Cell. 2014; 5(4): 258-260.
11. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. One-step generation of mice carrying reporter and conditional alleles by crispr/cas-mediated genome engineering. Cell. 2013; 154(6): 1370-1399.
12. Li K, Wang G, Andersen T, Zhou P, Pu WT. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. PLoS One. 2014; 9(8): e105779.
13. Hendel A, Kildebeck EJ, Fine EJ, Clark JT, Punjya N, Sebastiano V, et al. Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep. 2014; 7(1): 293-305.
14. Adams DJ, van der Weyden L. Contemporary approaches for modifying the mouse genome. Physiol Genomics. 2008; 34(3): 225-238.
15. Hassani SN, Totonchi M, Sharifi-Zarchi A, Mollamohammadi S, Pakzad M, Moradi S, et al. Inhibition of TGFβ Signaling Promotes Ground State Pluripotency. Stem Cell Rev. 2014; 10(1): 16-30.
16. Rassouli H, Nemati S, Rezaeiani S, Sayadmanesh A, Gharaati MR, Salekdeh GH, et al. Cloning, expression, and functional characterization of in-house prepared human leukemia inhibitory factor. Cell J. 2013; 15(2): 190-197.
17. Hassani SN, Totonchi M, Farrokhi A, Taei A, Larijani MR, Gourabi H, et al. Simultaneous suppression of TGF-β and ERK signaling contributes to the highly efficient and reproducible generation of mouse embryonic stem cells from previously considered refractory and non-permissive strains. Stem Cell Rev. 2012; 8(2): 472-481.
18. Tesson L, Heslan JM, Ménoret S, Anegon I. Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR. Transgenic Res. 2002; 11(1): 43-48.
19. Ballester M, Castelló A, Ibáñez E, Sánchez A, Folch JM. Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals. Biotechniques. 2004; 37(4): 610-613.
20. Ginzinger DG Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream. Exp Hematol. 2002; 30(6): 503-512.
21. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013; 8(11): 2281-2308.
22. Hasty P, Rivera-Pérez J, Bradley A. The role and fate of DNA ends for homologous recombination in embryonic stem cells. Mol Cell Biol. 1992; 12(6): 2464-2474.