Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR

Transgenic Research

Volumn 11, Issue 1, 2002, Pages 43-48

  Tesson, Laurent ^a   Heslan, Jean Marie ^a   Ménoret, Séverine ^a   Anegon, Ignacio ^a  

^a NANTES UNIVERSITY HOSPITAL   (France)

Author keywords

Rats; Real time PCR; Transgenic; Zygosity

Indexed keywords

ANIMALIA;

DECAY ACCELERATING FACTOR; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; PRIMER DNA;

ANIMAL; ARTICLE; DNA PROBE; GENE AMPLIFICATION; GENETICS; GENOTYPE; HUMAN; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RAT; REPRODUCIBILITY; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ANTIGENS, CD55; BASE SEQUENCE; DNA PRIMERS; DNA PROBES; GENE AMPLIFICATION; GENOTYPE; HUMANS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; POLYMERASE CHAIN REACTION; RATS; REPRODUCIBILITY OF RESULTS;

EID: 0036008336     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1013928600442     Document Type: Article

References (6)

1. Protection against hyperacute xenograft rejection of transgenic rat hearts expressing human decay accelerating factor (DAF) transplanted into primates
2. Screening of homozygous transgenic mice by comparative PCR
3. Real time quantitative PCR
4. Molecular approaches involved in mammalian gene transfer: Analysis of transgene integration