Efficient and regioselective synthesis of β-GalNAc/GlcNAc-lactose by a bifunctional transglycosylating β-N-acetylhexosaminidase from Bifidobacterium bifidum

Applied and Environmental Microbiology

Volumn 82, Issue 18, 2016, Pages 5642-5652

  Chen, Xiaodi ^a   Xu, Li ^a   Jin, Lan ^a   Sun, Bin ^a   Gu, Guofeng ^a   Lu, Lili ^a   Xiao, Min ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; ENZYME ACTIVITY; ESCHERICHIA COLI; GLYCOSYLATION; SUGARS;

BACTEROIDES FRAGILIS; BIFIDOBACTERIUM BIFIDUM; CLOSTRIDIUM PERFRINGENS; N-ACETYLHEXOSAMINIDASE; OLIGOSACCHARIDE SYNTHESIS; REGIOSELECTIVE SYNTHESIS; SUBSTRATE CONCENTRATIONS; TRANS GLYCOSYLATION ACTIVITY;

REGIOSELECTIVITY;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; NUMERICAL MODEL; PH; PROTEIN; SUGAR;

BACTEROIDES FRAGILIS NCTC 9343; BIFIDOBACTERIUM BIFIDUM; CLOSTRIDIUM PERFRINGENS ATCC 13124; ESCHERICHIA COLI;

BETA N ACETYLHEXOSAMINIDASE; LACTOSE; N ACETYLGALACTOSAMINE; N ACETYLGLUCOSAMINE; RECOMBINANT PROTEIN;

BACTEROIDES FRAGILIS; BIFIDOBACTERIUM BIFIDUM; CHEMISTRY; CLOSTRIDIUM PERFRINGENS; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR DOCKING; PH; TEMPERATURE;

ACETYLGALACTOSAMINE; ACETYLGLUCOSAMINE; BACTEROIDES FRAGILIS; BETA-N-ACETYLHEXOSAMINIDASES; BIFIDOBACTERIUM BIFIDUM; CLONING, MOLECULAR; CLOSTRIDIUM PERFRINGENS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; KINETICS; LACTOSE; MOLECULAR DOCKING SIMULATION; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 84987850886     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01325-16     Document Type: Article

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