Backbone structures in human milk oligosaccharides: trans-glycosylation by metagenomic β-N-acetylhexosaminidases

Applied Microbiology and Biotechnology

Volumn 99, Issue 19, 2015, Pages 7997-8009

  Nyffenegger, Christian ^a   Nordvang, Rune Thorbjørn ^a   Zeuner, Birgitte ^a   Łężyk, Mateusz ^a   Difilippo, Elisabetta ^b   Logtenberg, Madelon J ^b   Schols, Henk A ^b   Meyer, Anne S ^a   Mikkelsen, Jørn Dalgaard ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Chito oligosaccharides; Functional screening; Lacto N triose II; Protein expression; Synthetic biology

Indexed keywords

AFFINITY CHROMATOGRAPHY; ENZYMES; ESCHERICHIA COLI; ESTERIFICATION; GLUCOSE; GLYCOSYLATION; HYDROLYSIS; PROTEINS; SACCHARIFICATION; SUGARS;

DEGREE OF POLYMERIZATION; FUNCTIONAL SCREENING; HUMAN MILK OLIGOSACCHARIDES; LACTO-N-TRIOSE II; METAGENOMIC LIBRARIES; PROTEIN EXPRESSIONS; SYNTHETIC BIOLOGY; TRANS-GLYCOSYLATION;

OLIGOSACCHARIDES;

BETA N ACETYLHEXOSAMINIDASE; GALACTOSE; GLUCOSE; GLYCOSIDASE; HEX1 ENZYME; HEX2 ENZYME; LACTOSE; MALTOSE; NICKEL; OLIGOSACCHARIDE; SUCROSE; TRIOSE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN;

BIOTECHNOLOGY; CHITIN; ENZYME ACTIVITY; GENE EXPRESSION; GENOMICS; HOMINID; MILK; MOLECULAR ANALYSIS; MUSCLE; NICKEL; POLYSACCHARIDE; PROTEIN;

AFFINITY CHROMATOGRAPHY; ALGIBACTER LECTUS; ARTICLE; BACTERIUM; BREAST MILK; CHEMICAL STRUCTURE; CLONING; ESCHERICHIA COLI; FOSMID; GLYCOSYLATION; HYDROLYSIS; METAGENOMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERIZATION; PROTEIN EXPRESSION; SOIL; STREPTOMYCES SCABIEI; AMINO ACID SEQUENCE; CHEMISTRY; CLASSIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; SEQUENCE ALIGNMENT;

ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BACTERIA; BACTERIAL PROTEINS; BETA-N-ACETYLHEXOSAMINIDASES; ENZYME STABILITY; GLYCOSYLATION; HUMANS; METAGENOMICS; MILK, HUMAN; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PHYLOGENY; SEQUENCE ALIGNMENT; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY;

EID: 84941181658     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6550-0     Document Type: Article

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