CRISPR/Cas-mediated site-specifi c mutagenesis in arabidopsis thaliana using Cas9 nucleases and paired nickases

Methods in Molecular Biology

Volumn 1469, Issue , 2016, Pages 111-122

  Schiml, Simon ^a   Fauser, Friedrich ^a   Puchta, Holger ^a  

^a NONE

Author keywords

Double strand break repair; Engineered nucleases; Gene technology; Genome engineering; Targeted mutagenesis

Indexed keywords

CAS9 NICKASE; CAS9 NUCLEASE; ENZYME; NUCLEASE; PLANT RNA; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; DEOXYRIBONUCLEASE I; ENDONUCLEASE; GUIDE RNA;

ARABIDOPSIS THALIANA; CRISPR CAS SYSTEM; EXPRESSION VECTOR; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; SITE DIRECTED MUTAGENESIS; ARABIDOPSIS; GENE VECTOR; GENETICS; METABOLISM; MUTATION; PROCEDURES;

ARABIDOPSIS; BACTERIAL PROTEINS; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; ENDONUCLEASES; GENETIC VECTORS; MUTAGENESIS, SITE-DIRECTED; MUTATION; RNA, GUIDE;

EID: 84984874849     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-4931-1_8     Document Type: Chapter

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