Expression, purification and biochemical characterization of AtFUT1, a xyloglucan-specific fucosyltransferase from Arabidopsis thaliana

Biochimie

Volumn 128-129, Issue , 2016, Pages 183-192

  Cicéron, Félix ^a   Rocha, Joana ^a   Kousar, Sumaira ^a   Hansen, Sara Fasmer ^a   Chazalet, Valérie ^a   Gillon, Emilie ^a   Breton, Christelle ^a   Lerouxel, Olivier ^a  

^a UNIV GRENOBLE ALPES   (France)

Author keywords

AtFUT1; Fucosyltransferase; Insect cell expression; ITC; Xyloglucan

Indexed keywords

FUCOSYLTRANSFERASE; FUCOSYLTRANSFERASE 1; GLUCAN; HOMODIMER; UNCLASSIFIED DRUG; ARABIDOPSIS PROTEIN; GUANOSINE DIPHOSPHATE FUCOSE; PROTEIN BINDING; RECOMBINANT PROTEIN; SOLUTION AND SOLUBILITY; XYLAN; XYLOGLUCAN; XYLOGLUCAN 2-FUCOSYLTRANSFERASE;

ARABIDOPSIS THALIANA; ARTICLE; BIOCHEMICAL ANALYSIS; BIOLUMINESCENCE; CARBOHYDRATE SYNTHESIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; INSECT CELL; INSECT CELL CULTURE; ISOTHERMAL TITRATION CALORIMETRY; NONHUMAN; PROTEIN EXPRESSION; TAMARIND; ANIMAL; ARABIDOPSIS; CELL LINE; CHEMISTRY; CYTOLOGY; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY; METABOLISM; MOTH; PROTEIN MULTIMERIZATION; SIZE EXCLUSION CHROMATOGRAPHY; SOLUTION AND SOLUBILITY; WESTERN BLOTTING;

ANIMALS; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, GEL; FUCOSYLTRANSFERASES; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUCANS; GUANOSINE DIPHOSPHATE FUCOSE; MOTHS; PROTEIN BINDING; PROTEIN MULTIMERIZATION; RECOMBINANT PROTEINS; SOLUTIONS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY; XYLANS;

EID: 84984788885     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2016.08.012     Document Type: Article

References (36)

1. [1] Atmodjo, M.A., Hao, Z., Mohnen, D., Evolving views of pectin biosynthesis. Annu. Rev. Plant Biol. 64 (2013), 747–779.
2. [2] Oikawa, A., Lund, C.H., Sakuragi, Y., Scheller, H.V., Golgi-localized enzyme complexes for plant cell wall biosynthesis. TIPS 18 (2013), 49–58.
3. [3] Carpita, N.C., Progress in the biological synthesis of the plant cell wall: new ideas for improving biomass for bioenergy. Curr. Opin. Biotechnol. 23 (2012), 330–337.
4. [4] Pauly, M., Keegstra, K., Cell-wall carbohydrates and their modification as a resource for biofuels. Plant J. 54 (2008), 559–568.
5. [5] Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K., Plant cell walls to ethanol. Biochem. J. 442 (2012), 241–252.
6. [6] Mishra, A., Malhotra, A.V., Tamarind xyloglucan: a polysaccharide with versatile application potential. J. Mater. Chem. 19 (2009), 8528–8536.
7. [7] Mazzarino, L., Loch-Neckel, G., Dos Santos Bubniak, L., Ourique, F., Otsuka, I., Halila, S., Curi Pedrosa, R., Santos-Silva, M.C., Lemos-Senna, E., Curti Muniz, E., Borsali, R., Nanoparticles made from xyloglucan-block-polycaprolactone copolymers: safety assessment for drug delivery. Toxicol. Sci. 147 (2015), 104–115.
8. [8] Burton, R.A., Fincher, G.B., Plant cell wall engineering: applications in biofuel production and improved human health. Curr. Opin. Biotechnol. 26 (2014), 79–84.
9. [9] De Bruyn, F., Maertens, J., Beauprez, J., Soetaert, W., De Mey, M., Biotechnological advances in UDP-sugar based glycosylation of small molecules. Biotechnol. Adv. 33 (2015), 288–302.
10. [10] Rocha, J., Audry, M., Pesce, G., Chazalet, V., Block, M.A., Maréchal, E., Breton, C., Revisiting the expression and purification of MGD1, the major galactolipid synthase in Arabidopsis to establish a novel standard for biochemical and structural studies. Biochimie 95 (2013), 700–708.
11. [11] Perrin, R.M., DeRocher, A.E., Bar-Peled, M., Zeng, W., Norambuena, L., Orellana, A., Raikhel, N.V., Keegstra, K., Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis. Science 284 (1999), 1976–1979.
12. [12] Madson, M., Dunand, C., Li, X., Verma, R., Vanzin, G.F., Caplan, J., Shoue, D.A., Carpita, N.C., Reiter, W.D., The MUR3 gene of Arabidopsis encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins. Plant Cell. 15 (2003), 1662–1670.
13. [13] Egelund, J., Petersen, B.L., Motawia, M.S., Damager, I., Faik, A., Olsen, C.E., Ishii, T., Clausen, H., Ulvskov, P., Geshi, N., Arabidopsis thaliana RGXT1 and RGXT2 encode Golgi-localized (1,3)-alpha-D-xylosyltransferases involved in the synthesis of pectic rhamnogalacturonan-II. Plant Cell 18 (2006), 2593–25607.
14. [14] Sterling, J.D., Atmodjo, M.A., Inwood, S.E., Kumar Kolli, V.S., Quigley, H.F., Hahn, M.G., Mohnen, D., Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase. Proc. Natl. Acad. Sci. U. S. A. 103 (2006), 5236–5241.
15. [15] Cavalier, D.M., Keegstra, K., Two xyloglucan xylosyltransferases catalyze the addition of multiple xylosyl residues to cellohexaose. J. Biol. Chem. 281 (2006), 34197–34207.
16. [16] Lerouxel, O., Cavalier, D.M., Liepman, A.H., Keegstra, K., Biosynthesis of plant cell wall polysaccharides-a complex process. Curr. Opin. Plant Biol. 9 (2006), 621–630.
17. [17] Faik, A., Bar-Peled, M., DeRocher, A.E., Zeng, W., Perrin, R.M., Wilkerson, C., Raikhel, N.V., Keegstra, K., Biochemical characterization and molecular cloning of an alpha-1,2-fucosyltransferase that catalyzes the last step of cell wall xyloglucan biosynthesis in pea. J. Biol. Chem. 275 (2000), 15082–15089.
18. [18] Vanzin, G.F., Madson, M., Carpita, N.C., Raikhel, N.V., Keegstra, K., Reiter, W.D., The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1. Proc. Natl. Acad. Sci. U. S. A. 99 (2002), 3340–3345.
19. [19] Possee, R.D., Baculoviruses as expression vectors. Curr. Opin. Biotechnol. 5 (1997), 569–572.
20. [20] Münster, J., Ziegelmüller, P., Spillner, E., Bredehorst, R., High level expression of monomeric and dimeric human alpha1,3-fucosyltransferase V. J. Biotechnol. 121 (2006), 448–457.
21. [21] Ihara, H., Okada, T., Ikeda, Y., Cloning, expression and characterization of Bombyx mori α1,6-fucosyltransferase. Biochem. Biophys. Res. Commun. 450 (2014), 953–960.
22. [22] Spadiut, O., Ibatullin, F.M., Peart, J., Gullfot, F., Martinez-Fleites, C., Ruda, M., Xu, C., Sundqvist, G., Davies, G.J., Brumer, H., Building custom polysaccharides in vitro with an efficient, broad-specificity xyloglucan glycosynthase and a fucosyltransferase. J. Am. Chem. Soc. 133 (2011), 10892–10900.
23. [23] Kelley, L.A., Mezulis, S., Yates, C.M., Wass, M.N., Sternberg, M.J., The Phyre2 web portal for protein modeling, prediction and analysis. Nat. Protoc. 10 (2015), 845–858.
24. [24] Ferre, F., Clote, P., DiANNA: a web server for disulfide connectivity prediction. Nucleic Acids Res. 33 (2005), W230–W232 (Web Server issue).
25. [25] Fry, S.C., York, W.S., Albersheim, P., An unambiguous nomenclature for xyloglucan-derived oligosaccharide. Physiol. Plant. 89 (1993), 1–3.
26. [26] Zabotina, O., Xyloglucan and its biosynthesis. Front. Plant Sci. 3 (2012), 1–5.
27. [27] Pauly, M., Gille, S., Liu, L., Mansoori, N., de Souza, A., Schultnik, A., Xiong, G., Hemicellulose biosynthesis. Planta 238 (2013), 627–642.
28. [28] Chou, Y.H., Pogorelko, G., Young, Z.T., Zabotina, O., Protein-protein interactions among xyloglucan-synthesizing enzymes and formation of Golgi-localized multiprotein complexes. Plant Cell Physiol. 56 (2015), 255–267.
29. [29] Chou, Y.H., Pogorelko, G., Zabotina, O.A., Xyloglucan xylosyltransferases XXT1, XXT2, and XXT5 and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes. Plant Physiol. 159 (2012), 1355–1366.
30. [30] Lund, C.H., Bromley, J.R., Stenbæk, A., Rasmussen, R.E., Scheller, H.V., Sakuragi, Y., A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus. J. Exp. Bot. 66 (2015), 85–97.
31. [31] Breton, C., Snajdrová, L., Jeanneau, C., Koca, J., Imberty, A., Structures and mechanisms of glycosyltransferases. Glycobiology 16 (2006), 29R–37R.
32. [32] Sindhuwinata, N., Grimm, L.L., Weißbach, S., Zinn, S., Munoz, E., Palcic, M.M., Peters, T., Thermodynamic signature of substrates and substrate analogs binding to human blood group B galactosyltransferase from isothermal titration calorimetry experiments. Biopolymers 99 (2015), 784–795.
33. [33] Toki, D., Sarkar, M., Yip, B., Reck, F., Joziasse, D., Fukuda, M., Schachter, H., Brockhausen, I., Expression of stable human O-glycan core 2 beta-1,6-N-acetylglucosaminyltransferase in Sf9 insect cells. Biochem. J. 325 (1997), 63–69.
34. [34] Tessier, D.C., Thomas, D.Y., Khouri, H.E., Laliberte, F., Vernet, T., Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide. Gene 98 (1991), 177–183.
35. [35] Reed, L.J., Muench, H.A., Simple method of estimating fifty percent endpoints. Am. J. Hyg. 27 (1938), 493–497.
36. [36] Lerouxel, O., Choo, T.S., Séveno, M., Usabel, B., Faye, L., Lerouge, P., Pauly, M., Rapid structural phenotyping of plant cell wall mutants by enzymatic oligosaccharide fingerprinting. Plant Physiol. 130 (2002), 1754–1763.