Revisiting the expression and purification of MGD1, the major galactolipid synthase in Arabidopsis to establish a novel standard for biochemical and structural studies

Biochimie

Volumn 95, Issue 4, 2013, Pages 700-708

  Rocha, Joana ^a   Audry, Magali ^a   Pesce, Gaelle ^a   Chazalet, Valérie ^a   Block, Maryse A ^b   Maréchal, Eric ^b   Breton, Christelle ^a  

^a CENTRE DE RECHERCHES SUR LES MACROMOLÉCULES VÉGÉTALES CERMAV CNRS   (France)

^b Laboratoire de Physiologie Cellulaire Vegetale Grenoble   (France)

Author keywords

Galactolipid; Galactolipid synthase; Phosphatidic acid; Phosphatidylglycerol

Indexed keywords

MGD1 PROTEIN; SYNTHETASE; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; BIOCHEMISTRY; CRYSTALLIZATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HOST CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PROTEIN AGGREGATION; PROTEIN EXPRESSION;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CATALYTIC DOMAIN; CRYSTALLIZATION; ESCHERICHIA COLI; GALACTOSYLTRANSFERASES; GENE EXPRESSION; GENETIC ENGINEERING;

ALGAE; ARABIDOPSIS; ESCHERICHIA COLI;

EID: 84874664872     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2012.11.011     Document Type: Article

References (24)

1. H. Härtel, P. Dormann, and C. Benning DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis Proc. Natl. Acad. Sci. U. S. A. 97 2000 10649 10654
2. J. Jouhet, E. Marechal, B. Baldan, R. Bligny, J. Joyard, and M.A. Block Phosphate deprivation induces transfer of DGDG galactolipid from chloroplast to mitochondria J. Cell. Biol. 167 2004 863 874
3. M.A. Block, A.J. Dorne, J. Joyard, and R. Douce Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. II. Biochemical characterization J. Biol. Chem. 258 1983 13281 13286
4. C. Benning, and H. Ohta Three enzyme systems for galactoglycerolipid biosynthesis are coordinately regulated in plants J. Biol. Chem. 280 2005 2397 2400
5. C. Miège, E. Maréchal, M. Shimojima, K. Awai, M.A. Block, H. Ohta, K. Takamiya, R. Douce, and J. Joyard Biochemical and topological properties of type A MGDG synthase, a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG Eur. J. Biochem. 265 1999 990 1001
6. L. Boudière, C.Y. Botté, N. Saidani, M. Lajoie, J. Marion, L. Bréhélin, Y. Yamaryo-Botté, B. Satiat-Jeunemaître, C. Breton, A. Girard-Egrot, O. Bastien, J. Jouhet, D. Falconet, M.A. Block, and E. Maréchal Mol. Biosyst. 8 2012 2023 2035
7. K. Awai, E. Maréchal, M.A. Block, D. Brun, T. Masuda, H. Shimada, K. Takamiya, H. Ohta, and J. Joyard Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana Proc. Natl. Acad. Sci. U. S. A. 98 2001 10960 10965
8. J.E. Froehlich, C. Benning, and P. Dörmann The digalactosyldiacylglycerol (DGDG) synthase DGD1 is inserted into the outer envelope membrane of chloroplasts in a manner independent of the general import pathway and does not depend on direct interaction with monogalactosyldiacylglycerol synthase for DGDG biosynthesis J. Biol. Chem. 276 2001 31806 31812
9. K. Kobayashi, M. Kondo, H. Fukuda, M. Nishimura, and H. Ohta Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis Proc. Natl. Acad. Sci. U. S. A. 104 2007 17216 17221
10. C.Y. Botté, M. Deligny, A. Roccia, A.L. Bonneau, N. Saïdani, H. Hardré, S. Aci, Y. Yamaryo-Botté, J. Jouhet, E. Dubots, K. Loizeau, O. Bastien, L. Bréhélin, J. Joyard, J.C. Cintrat, D. Falconet, M.A. Block, B. Rousseau, R. Lopez, and E. Maréchal Chemical inhibitors of monogalactosyldiacylglycerol synthases in Arabidopsis thaliana Nat. Chem. Biol. 7 2011 834 842
11. E. Maréchal, C. Miège, M.A. Block, R. Douce, and J. Joyard The catalytic site of monogalactosyldiacylglycerol synthase from spinach chloroplast envelope membranes. Biochemical analysis of the structure and of the metal content J. Biol. Chem. 270 1995 5714 5722
12. S. Ha, D. Walker, Y. Shi, and S. Walker The 1.9 A crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis Protein Sci. 9 2000 1045 1052
13. Y. Hu, L. Chen, S. Ha, B. Gross, B. Falcone, D. Walker, M. Mokhtarzadeh, and S. Walker Crystal structure of the MurG: UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases Proc. Natl. Acad. Sci. U. S. A. 100 2003 845 849
14. C. Breton, S. Fournel-Gigleux, and M.M. Palcic Recent structures, evolution and mechanisms of glycosyltransferases Curr. Opin. Struct. Biol. 22 2012 540 549
15. C. Botté, C. Jeanneau, L. Snajdrova, O. Bastien, A. Imberty, C. Breton, and E. Maréchal Molecular modeling and site-directed mutagenesis of plant chloroplast monogalactosyldiacylglycerol synthase reveal critical residues for activity J. Biol. Chem. 280 2005 34691 34701
16. E. Dubots, M. Audry, Y. Yamaryo, O. Bastien, H. Ohta, C. Breton, E. Maréchal, and M.A. Block Activation of the chloroplast monogalactosyldiacylglycerol synthase MGD1 by phosphatidic acid and phosphatidylglycerol J. Biol. Chem. 285 2010 6003 6011
17. Y. Nishiyama, H. Hardré-Liénard, S. Miras, C. Miège, M.A. Block, F. Revah, J. Joyard, and E. Maréchal Refolding from denatured inclusion bodies, purification to homogeneity and simplified assay of MGDG synthases from land plants Protein Expr. Purif. 31 2003 79 87
18. Y. Kim, G. Babnigg, R. Jedrzejczak, W.H. Eschenfeldt, H. Li, N. Maltseva, C. Hatzos-Skintges, M. Gu, M. Makowska-Grzyska, R. Wu, H. An, G. Chhor, and A. Joachimiak High-throughput protein purification and quality assessment for crystallization Methods 55 2011 12 28
19. K. Houben, D. Marion, N. Tarbouriech, R.W. Ruigrok, and L. Blanchard Interaction of the C-terminal domains of sendai virus N and P proteins: comparison of polymerase-nucleocapsid interactions within the paramyxovirus family J. Virol. 81 2007 6807 6816
20. E.G. Bligh, and W.J. Dyer A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 37 1959 911 917
21. S.E. Bondos, and A. Bicknell Detection and prevention of protein aggrhation before, during, and after purification Anal. Biochem. 316 2003 223 231
22. U.B. Ericsson, B.M. Hallberg, G.T. Detitta, N. Dekker, and P. Nordlund Thermofluor-based high-throughput stability optimization of proteins for structural studies Anal. Biochem. 357 2006 289 298
23. F. Dupeux, M. Röwer, G. Seroul, D. Blot, and J.A. Márquez A thermal stability assay can help to estimate the crystallization likelihood of biological samples Acta Crystallogr. D: Biol. Crystallogr. 67 2011 915 919
24. J. Covés, J. Joyard, and R. Douce Lipid requirement and kinetic studies of solubilized UDP-galactose:diacylglycerol galactosyltransferase activity from spinach chloroplast envelope membranes Proc. Natl. Acad. Sci. U. S. A. 85 1988 4966 4970