Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Zhou, Mengying ^a   Zhang, Xulang ^b   Wen, Xinyu ^a   Wu, Taihua ^a   Wang, Weidong ^a   Yang, Mingzhou ^c   Wang, Jing ^a   Fang, Ming ^a   Lin, Bingcheng ^b   Lin, Hongli ^a  

^a DALIAN MEDICAL UNIVERSITY   (China)

^b DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^c Dalian Friendship Hospital   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; BETA 2 MICROGLOBULIN; IMMUNOGLOBULIN G;

ANIMAL; CELL ADHESION; CHRONIC KIDNEY FAILURE; COCULTURE; CYTOLOGY; CYTOSKELETON; GLOMERULUS; HUMAN; HYPERTENSION; IN VITRO STUDY; KIDNEY; KIDNEY DISEASE; LAB ON A CHIP; METABOLISM; NEPHRITIS; PATHOPHYSIOLOGY; PODOCYTE; RAT; RENOVASCULAR HYPERTENSION; SPONTANEOUSLY HYPERTENSIVE RAT;

ACTINS; ANIMALS; BETA 2-MICROGLOBULIN; CELL ADHESION; COCULTURE TECHNIQUES; CYTOSKELETON; HUMANS; HYPERTENSION; HYPERTENSION, RENAL; IMMUNOGLOBULIN G; IN VITRO TECHNIQUES; KIDNEY; KIDNEY DISEASES; KIDNEY FAILURE, CHRONIC; KIDNEY GLOMERULUS; LAB-ON-A-CHIP DEVICES; NEPHRITIS; PODOCYTES; RATS; RATS, INBRED SHR;

EID: 84983784148     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep31771     Document Type: Article

References (44)

1. Hart, P. D. & Bakris, G. L. Hypertensive nephropathy: prevention and treatment recommendations. Expert Opin Pharmacother 11, 2675-2686 (2010).
2. Tramonti, G. & Kanwar, Y. S. Review and discussion of tubular biomarkers in the diagnosis and management of diabetic nephropathy. Endocrine 43, 494-503 (2013).
3. Endlich, N. & Endlich, K. The challenge and response of podocytes to glomerular hypertension. Semin Nephrol 32, 327-341 (2012).
4. Ihm, C. G. Hypertension in Chronic Glomerulonephritis. Electrolyte Blood Press 13, 41-45 (2015).
5. Chiu, J. J. & Chien, S. Effects of disturbed flow on vascular endothelium: pathophysiological basis and clinical perspectives. Physiol Rev. 91, 327-387 (2011).
6. Huang, C., Bruggeman, L. A., Hydo, L. M. & Miller, R. T. Shear stress induces cell apoptosis via a c-Src-phospholipase D-mTOR signaling pathway in cultured podocytes. Exp Cell Res. 318, 1075-1085 (2012).
7. Hausmann, R. et al. Electrical forces determine glomerular permeability. J Am Soc Nephrol 21, 2053-2058 (2010).
8. Hung, L. Y. et al. Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX. Sci Rep. 5, 10326 (2015).
9. Maschmeyer, I. et al. A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents. Lab Chip. 15, 2688-2699 (2015).
10. Wang, G. et al. Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-onchip technologies. Nat Med. 20, 616-623 (2014).
11. Kim, D., Wu, X., Young, A. T. & Haynes, C. L. Microfluidics-based in vivo mimetic systems for the study of cellular biology. Acc Chem Res. 47, 1165-1173 (2014).
12. Huh, D., Hamilton, G. A. & Ingber, D. E. From 3D cell culture to organs-on-chips. Trends Cell Biol. 21, 745-754 (2011).
13. Bruggeman, L. A., Doan, R. P., Loftis, J., Darr, A. & Calabro, A. A cell culture system for the structure and hydrogel properties of basement membranes; Application to capillary walls. Cell Mol Bioeng. 5, 194-204 (2012).
14. Booth, R. & Kim, H. Characterization of a microfluidic in vitro model of the blood-brain barrier (muBBB). Lab Chip. 12, 1784-1792 (2012).
15. Frohlich, E. M., Zhang, X. & Charest, J. L. The use of controlled surface topography and flow-induced shear stress to influence renal epithelial cell function. Integr Biol (Camb) 4, 75-83 (2012).
16. Slater, S. C. et al. An in vitro model of the glomerular capillary wall using electrospun collagen nanofibres in a bioartificial composite basement membrane. Plos One 6, e20802 (2011).
17. Huh, D. et al. Reconstituting organ-level lung functions on a chip. Science 328, 1662-1668 (2010).
18. Camp, J. P. & Capitano, A. T. Induction of zone-like liver function gradients in HepG2 cells by varying culture medium height. Biotechnol Prog. 23, 1485-1491 (2007).
19. Wilmer, M. J. et al. Kidney-on-a-Chip Technology for Drug-Induced Nephrotoxicity Screening. Trends Biotechnol. 34, 156-170 (2016).
20. Kyung-Jin, Jang et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol (Camb). 5, 1119-1129 (2013).
21. Zhou, M., Ma, H., Lin, H. & Qin, J. Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices. Biomaterials 35, 1390-1401 (2014).
22. Li, K., Wang, J., Yin, X., Zhai, X. & Li, Z. Alteration of podocyte protein expression and localization in the early stage of various hemodynamic conditions. Int J Mol Sci. 14, 5998-6011 (2013).
23. Merscher-Gomez, S. et al. Cyclodextrin protects podocytes in diabetic kidney disease. Diabetes. 62, 3817-3827 (2013).
24. Denton, K. M., Anderson, W. P. & Sinniah, R. Effects of angiotensin II on regional afferent and efferent arteriole dimensions and the glomerular pole. Am J Physiol Regul Integr Comp Physiol. 279, 629-3 (2000).
25. Schermer, B. & Benzing, T. Lipid-protein interactions along the slit diaphragm of podocytes. J Am Soc Nephrol. 20, 473-478 (2009).
26. Schnittler, H. J. et al. Role of actin filaments in endothelial cell-cell adhesion and membrane stability under fluid shear stress. Pflugers Arch. 442, 675-687 (2001).
27. Shakhov, A. S., Verin, A. D. & Alieva, I. B. Endothelial cell cytoskeleton reorganization during functional monolayer formation in vitro. Tsitologiia. 56, 36-47 (2014).
28. Fu, X. et al. Shear stress-induced unfolding of VWF accelerates oxidation of key methionine residues in the A1A2A3 region. Blood 118, 5283-5291 (2011).
29. Bi, J., Pellenz, C. D. & Krendel, M. Visualization of cytoskeletal dynamics in podocytes using adenoviral vectors. Cytoskeleton (Hoboken) 71, 145-156 (2014).
30. Bi, J. et al. Myosin 1e is a component of the glomerular slit diaphragm complex that regulates actin reorganization during cell-cell contact formation in podocytes. Am J Physiol Renal Physiol. 305, F532-F544 (2013).
31. Tossidou, I. et al. CIN85/RukL is a novel binding partner of nephrin and podocin and mediates slit diaphragm turnover in podocytes. J Biol Chem. 285, 25285-25295 (2010).
32. Scott, R. P. & Quaggin, S. E. Review series: The cell biology of renal filtration. J Cell Biol. 209, 199-210 (2015).
33. Martín Alemany, N., Almirall Daly, J., Orellana Fernández, R. & Andreu, X. Nephrotic proteinuria in hypertensive nephrosclerosis: clinical and evolution characteristics. Med Clin (Barc) 143, 392-394 (2014).
34. Chia-Yi, Tseng. et al. Protective effects of N-Acetyl cysteine against diesel exhaust particles-induced intracellular ROS generates pro-inflammatory cytokines to mediate the vascular permeability of capillary-like endothelial tubes. Plos One 10, e0131911 (2015).
35. Mei, H. et al. Regulation of endothelial cell barrier function by antibody-driven affinity modulation of platelet endothelial cell adhesion molecule-1 (PECAM-1). J Biol Chem. 289, 20836-20844 (2014).
36. Poirault-Chassac, S. et al. Terminal platelet production is regulated by von Willebrand factor. Plos One. 8, e63810 (2013).
37. Sampogna, R. V. & Al-Awqati, Q. Taking a bite: endocytosis in the maintenance of the slit diaphragm. J Clin Invest. 122, 4330-4333 (2012).
38. Faul, C. et al. The actin cytoskeleton of kidney podocytes is a direct target of the antiproteinuric effect of cyclosporine A. Nat Med. 14, 931-938 (2008).
39. Wang, L. et al. Podocytes protect glomerular endothelial cells from hypoxic injury via deSUMOylation of HIF-1alpha signaling. Int J Biochem Cell Biol. 58, 17-27 (2015).
40. Oh, K. W., Lee, K., Ahn, B. & Furlani, E. P. Design of pressure-driven microfluidic networks using electric circuit analogy. Lab Chip. 12, 515-545 (2012).
41. Beebe, D. J., Mensing, G. A. & Walker, G. M. Physics and applications of microfluidics in biology. Annu Rev Biomed Eng 4, 261-286 (2002).
42. Bruus, H. Acoustofluidics 1: Governing equations in microfluidics. Lab Chip 11, 3742-3751 (2011).
43. Filippi-Chiela, E. C. et al. Nuclear morphometric analysis (NMA): screening of senescence, apoptosis and nuclear irregularities. Plos One 7, e42522 (2012).
44. He, Y. et al. Adenophorum induces cell cycle arrest and apoptosis of splenocytes through the mitochondrial Pathway and caspase Activation in saanen Goats. Sci Rep. 5, 15967 (2015).