Influence of 3D porous galactose containing PVA/gelatin hydrogel scaffolds on three-dimensional spheroidal morphology of hepatocytes

Journal of Materials Science: Materials in Medicine

Volumn 26, Issue 1, 2015, Pages

  Vasanthan, Kirthanashri S ^a   Subramaniam, Anuradha ^a   Krishnan, Uma Maheswari ^a   Sethuraman, Swaminathan ^a  

^a SASTRA UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROLIFERATION; CELLS; CYTOLOGY; PORE SIZE; SCANNING ELECTRON MICROSCOPY; TENSILE STRENGTH;

ALBUMIN SECRETION; FREEZE/THAW TECHNIQUE; HUMAN LIVER; HYDROGEL SCAFFOLDS; LIQUID DISPLACEMENTS; METABOLIC ACTIVITY; PORE INTERCONNECTIVITY; SCANNING ELECTRON MICROGRAPHS;

HYDROGELS;

ALBUMIN; GALACTOSE; GELATIN; POLYVINYL ALCOHOL; ALBUMINOID; BIOMATERIAL; CROSS LINKING REAGENT; HYDROGEL; POLYVINYL ACETATE; POLYVINYL DERIVATIVE;

ARTICLE; CELL AGGREGATION; CELL INTERACTION; CELL PROLIFERATION; CELL STRUCTURE; CONTROLLED STUDY; FREEZE THAWING; HUMAN; HUMAN CELL; HUMAN TISSUE; HYDROGEL; LIQUID DISPLACEMENT METHOD; LIVER CELL; MICROPHOTOGRAPHY; POROSITY; PRIORITY JOURNAL; PROCEDURES; SCANNING ELECTRON MICROGRAPH; TENSILE STRENGTH; THREE DIMENSIONAL SPHEROIDAL MORPHOLOGY; ANIMAL; BOVINE; CELL ADHESION; CELL LINEAGE; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; DIFFUSION; EXTRACELLULAR MATRIX; HEPG2 CELL LINE; LIVER; METABOLISM; PATHOLOGY; REGENERATION; SCANNING ELECTRON MICROSCOPY; TEMPERATURE; TISSUE ENGINEERING; TISSUE SCAFFOLD;

ALBUMINS; ANIMALS; BIOCOMPATIBLE MATERIALS; CATTLE; CELL ADHESION; CELL LINEAGE; CELL PROLIFERATION; CELL SURVIVAL; CROSS-LINKING REAGENTS; DIFFUSION; EXTRACELLULAR MATRIX; GALACTOSE; HEP G2 CELLS; HEPATOCYTES; HUMANS; HYDROGELS; LIVER; MICROSCOPY, ELECTRON, SCANNING; POLYVINYLS; POROSITY; REGENERATION; TEMPERATURE; TENSILE STRENGTH; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84983573790     PISSN: 09574530     EISSN: 15734838     Source Type: Journal    
DOI: 10.1007/s10856-014-5345-7     Document Type: Article

References (56)

1. Vasanthan KS, Subramanian A, Krishnan UM, Sethuraman S. Role of biomaterials, therapeutic molecules and cells for hepatic tissue engineering. Biotechnol Adv. 2012;30:742–52.
2. Chao YC, Ying L, Zhang PC, Zhuo RX, Kang ET, Leong KW, Ma HQ. High density of immobilized galactose ligand enhances hepatocyte attachment and function. J Biomed Mater Res A. 2003;67:1093–104.
3. Cho CS, Seo SJ, Park IK, Kim SH, Kim TH, Hoshiba T, Harada I, Akaike T. Galactose–carrying polymers as extracellular matrices for liver tissue engineering. Biomaterials. 2006;2:576–85.
4. Yang SM, Lee DH, Park JH. Effects of degree of cell-cell contact on liver specific functions of rat primary hepatocytes. Biotechnol Bioprocess Eng. 2000;5:99–105.
5. Geckil H, Xu F, Zhang X, Moon S, Demirci U. Engineering hydrogels as extracellular matrix mimics. Nanomedicine (Lond). 2010;5:469–84.
6. Kim M, Lee JY, Jones CN, Revzin A, Tae G. Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes. Biomaterials. 2010;31:3596–603.
7. Hwang CM, Sant S, Masaeli M, Kachouie NN, Zamanian B, Lee SH, Khademhosseini A. Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering. Biofabrication. 2010;2:035003–24.
8. Hago EE, Li X. Interpenetrating polymer network hydrogels based on gelatin and PVA by biocompatible approaches: synthesis and characterization. Adv Mater Sci Eng. 2013;2013:1–8.
9. Bhatia SN, Balis UJ, Yarmush ML, Toner M. Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells. FASEB J. 1999;13:1883–900.
10. Blum MM, Ovaert TC. Experimental and numerical tribological studies of a boundary lubricant functionalized poro-viscoelastic PVA hydrogel in normal contact and sliding. J Mech Behav Biomed Mater. 2012;14:248–58.
11. Jiang S, Liu S, Feng W. PVA hydrogel properties for biomedical application. J Mech Behav Biomed Mater. 2011;4:1228–33.
12. Oe S, Fukunaka Y, Hirose T, Yamaoka Y, Tabata Y. A trial on regeneration therapy of rat liver cirrhosis by controlled release of hepatocyte growth factor. J Control Release. 2003;88:193–200.
13. Wang X, Yan Y, Pan Y, Xiong Z, Liu H, Chang J, Liu F, Lin F, Wu R, Zhang R, Lu Q. Generation of three-dimensional hepatocyte/gelatin structures with rapid prototyping system. Tissue Eng. 2006;12:83–90.
14. Chung TW, Yang J, Akaike T, Cho KY, Nah JW, Kim SI, Cho CS. Preparation of alginate/galactosylated chitosan scaffold for hepatocyte attachment. Biomaterials. 2002;23:2827–34.
15. Ambury RF, Merry CLR, Ulijn RV. Sugar functionalised PEGA surfaces support metabolically active hepatocytes. J Mater Chem. 2011;21:2901–8.
16. Lang R, Stern MM, Smith L, Liu Y, Bharadwaj S, Liu G, Baptista PM, Bergman CR, Soker S, Yoo JJ, Atala A, Zhang Y. Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix. Biomaterials. 2011;32:7042–52.
17. Breslow JL, Sloan HR, Ferrans VJ, Anderson JL, Levy RI. Characterization of the mouse liver cell lineFL83B. Exp Cell Res. 1973;78:441–53.
18. Yin C, Liao K, Mao HQ, Leong KW, Zhuo RX, Chan V. Adhesion contact dynamics of HepG2 cells on galactose immobilized substrates. Biomaterials. 2003;24:837–50.
19. van de Kerkhove MP, Hoekdtra R, Chamuleau RA, van Gulik TM. Clinical application of bioartificial liver support systems. Ann Surg. 2004;240:216–30.
20. Chamuleau RA. Future of bioartificial liver support. World J Gastrointest Surg. 2009;30:21–5.
21. Zhao LF, Pan XP, Li LJ. Key challenges to the development of extracorporeal bioartificial liver support systems. Hepatobiliary Pancreat Dis Int. 2012;11:243–9.
22. Stockmann HB, Hiemstra CA, Marquet RL, IJzermans JN. Extracorporeal perfusion for the treatment of acute liver failure. Ann Surg. 2000;231:460–70.
23. Subramanian A, Krishnan UM, Sethuraman S. Fabrication of uniaxially aligned 3D electrospun scaffolds for neural regeneration. Biomed Mater. 2011;6:25004–14.
24. Sankaran K, Vasanthan KS, Krishnan UM, Sethuraman S. Development & evaluation of axially aligned nanofibres for blood vessel tissue engineering. J Tissue Eng Regen Med. 2012; doi:10.1002/term.1566.
25. Sethuraman S, Nair LS, El-Amin S, Nguyen MT, Singh A, Greish YE, Allcock HR, Brown PW, Laurencin CT. Development and characterization of biodegradable nanocomposite injectables for orthopaedic applications based on polyphosphazenes. J Biomater Sci Polym Ed. 2011;22:733–52.
26. Kuppan P, Vasanthan KS, Sundaramurthi D, Krishnan UM, Sethuraman S. Development of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibres for skin tissue engineering: effects of topography, mechanical and chemical stimuli. Biomacromolecules. 2011;12:3156–65.
27. Mandal BB, Kundu SC. Cell proliferation and migration in silk fibroin 3D scaffolds. Biomaterials. 2009;30:2956–65.
28. Liu Y, Geever LM, Kennedy JE, Higginbotham CL, Cahill PA, McGuinness GB. Thermal behavior and mechanical properties of physically crosslinked PVA/gelatin hydrogels. J Mech Behav Biomed Mater. 2010;3:203–9.
29. Rault J, Neffati R, Judeinstein P. Melting of ice in porous glass: why water and solvents confined in small pores do not crystallize. Eur Phys J. 2003;36:627–37.
30. Park KH. Improved long-term culture of hepatocytes in a hydrogel containing Arg-Gly-Asp (RGD). Biotechnol Lett. 2002;24:1131–5.
31. Ratner BD, Hoffman AS, Schoen FJ, Lemons JE. Biomaterials science: introduction to materials in medicine. 2nd ed. New York: Academic Press; 2004.
32. Lanza RP, Langer R, Vacanti J. Principles of tissue engineering. San Diego: Academic Press; 2000.
33. Slaughter BV, Khurshid SS, Fisher OZ, Khademhosseini A, Peppas NA. Hydrogels in regenerative medicine. Adv Mater. 2009;21:3307–29.
34. Huang H, Oizumi S, Kojima N, Niino T, Sakai Y. Avidin-biotin binding based cell seeding and perfusion culture of liver-derived cells in a porous scaffold with a three-dimensional interconnected flow-channel network. Biomaterials. 2007;28:3815–23.
35. Markus JB. Multiscale aspects of mechanical properties of biological materials. J Mech Behav Biomed Mater. 2011;4:125–7.
36. Wells RG. The role of matrix stiffness in regulating cell behavior. Hepatology. 2008;47:1394–400.
37. Lozoya OA, Wauthier E, Turner RA, Barbier C, Prestwich GD, Guilak F, Superfine R, Lubkin SR, Reid LM. Regulation of hepatic stem/progenitor phenotype by microenvironment stiffness in hydrogel models of the human liver stem cell niche. Biomaterials. 2011;32:7389–402.
38. McKee CT, Last JA, Russell P, Murphy CJ. Tissue Eng Part B Rev. 2011;17:155–64.
39. Zhao L, Chang J, Zhai W. Preparation and HL-7702 cell functionality of titania/chitosan composite scaffolds. J Mater Sci Mater Med. 2009;20:949–57.
40. Blakeney BA, Tambralli A, Anderson JM, Andukuri A, Lim DJ, Dean DR, Jun HW. Cell infiltration and growth in a low density, uncompressed three dimensional electrospun nanofibrous scaffold. Biomaterials. 2011;32:1583–90.
41. Oh SH, Kang SG, Kim ES, Cho SH, Lee JH. Fabrication and characterization of hydrophilic poly(lactic-co-glycolic acid)/poly(vinyl alcohol) blend cell scaffolds by melt-molding particulate-leaching method. Biomaterials. 2003;24:4011–21.
42. Meindl-Beinker NM, Dooley S. Transforming growth factor-β and hepatocyte transdifferentiation in liver fibrogenesis. J Gasteroenterol Hepatol. 2008;23:S122–7.
43. Richter JR, de Guzman RC, Van Dyke ME. Mechanism of hepatocyte attachment to keratin biomaterials. Biomaterials. 2011;32:7555–61.
44. Sanli O, Orhan E, Asman G. Release of salicylic acid through poly (vinyl alcohol/poly (vinyl pyrrolidone) and poly(vinyl alcohol-g-N-vinyl-2-pyrrolidone) membranes. J Appl Polym Sci. 2006;102:1244–53.
45. Paul W, Sharma CP. Polyetherurethaneurea reinforced poly (vinyl alcohol) dialysis membranes-studies on permeability and mechanical strength. Bull Mater Sci. 1994;17:1065–70.
46. Nakatsuka S, Andrady AL. Permeability of vitamin-B12 in chitosan membranes-effect of cross-linking and blending with poly(vinyl alcohol) on permeability. J Appl Polym Sci. 1992;44:17–28.
47. Shearer H, Ellis MJ, Perera SP, Chaudhuri JB. Effects of common sterilization methods on the structure and properties of poly(dl lactic-co-glycolic acid) scaffolds. Tissue Eng. 2006;12:2717–27.
48. Ricciardi R, Auriemma F, De Rosa C, Laupretre F. X-ray diffraction analysis of poly (vinyl alcohol) hydrogels, obtained by freezing and thawing techniques. Macromolecules. 2004;37:1921–7.
49. Katz JS, Burdick JA. Hydrogel mediated delivery of trophic factors for neural repair. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2009;1:128–39.
50. Chen JP, Cheng TH. Preparation and evaluation of thermo-reversible copolymer hydrogels containing chitosan and hyaluronic acid as injectable cell carriers. Polymer. 2009;50:107–16.
51. Linh NTB, Min YK, Song HY, Lee BT. Fabrication of polyvinyl alcohol/gelatin nanofiber composites and evaluation of their material properties. J Biomed Mater Res B Appl Biomater. 2010;95:184–91.
52. Vrana NE, O’Grady A, Kay E, Cahill PA, Mc Guinness B. Cell encapsulation within PVA-based hydrogels via freeze-thawing: a one-step scaffold formation and cell storage technique. J Tissue Eng Regen Med. 2009;3:567–72.
53. Du Y, Chia SM, Han R, Chang S, Tang H, Yu H. 3D hepatocyte monolayer on hybrid RGD/galactose substratum. Biomaterials. 2006;27:5669–80.
54. Billiet T, Gevaert E, De Schryver T, Cornelissen M, Dubrue P. The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials. 2014;35:49–62.
55. Murphy CM, O’Brien FJ. Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds. Cell Adhes Migr. 2010;4:377–81.
56. Wu Y, Zhao Z, Guan Y, Zhang Y. Galactosylated reversible hydrogels as scaffold for HepG2 spheroid generation. Acta Biomater. 2014;10:1965–74.