Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix

Biomaterials

Volumn 32, Issue 29, 2011, Pages 7042-7052

  Lang, Ren ^a,b   Stern, Matthew M ^b,c,d   Smith, Leona ^b   Liu, Yan ^b,e   Bharadwaj, Shantaram ^b   Liu, Guihua ^b   Baptista, Pedro M ^b   Bergman, Christopher R ^b   Soker, Shay ^b   Yoo, James J ^b   Atala, Anthony ^b   Zhang, Yuanyuan ^b  

^a CAPITAL MEDICAL UNIVERSITY   (China)

^b WAKE FOREST SCHOOL OF MEDICINE   (United States)

^c WAKE FOREST UNIVERSITY HEALTH SCIENCES   (United States)

^d UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

^e SICHUAN UNIVERSITY   (China)

Author keywords

3D; Albumin; Cell culture; ECM (extracellular matrix); Hepatocyte; Liver

Indexed keywords

3D; ALBUMIN; CELL FUNCTIONS; CELL TYPES; CELLULAR COMPONENTS; CELLULAR INFILTRATION; COLLAGEN GELS; CULTURE CONDITIONS; CULTURE SUBSTRATE; DECELLULARIZATION; DRUG DISCOVERY; ECM (EXTRACELLULAR MATRIX); EXTRACELLULAR MATRICES; GLYCOSAMINOGLYCANS; HEPATOCYTE; HEPATOCYTES; HEPG2 CELLS; HUMAN HEPATOCYTES; LAMININ; LIVER CELLS; LIVER TISSUE; MOLECULAR COMPONENTS; PHYSIOLOGICAL FUNCTIONS; PROTEOGLYCANS; THREE-DIMENSIONAL (3D); THREE-DIMENSIONAL CULTURE;

ANIMAL CELL CULTURE; CELLS; COLLAGEN; GLYCOPROTEINS; GROWTH KINETICS; OPTIMIZATION; POLYSTYRENES; THREE DIMENSIONAL;

TISSUE;

ALBUMIN; COLLAGEN GEL; COLLAGEN TYPE 1; COLLAGEN TYPE 3; COLLAGEN TYPE 4; ELASTIN; FIBRONECTIN; GLYCOSAMINOGLYCAN; LAMININ; POLYSTYRENE; PROTEOGLYCAN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL EXPANSION; CELL FUNCTION; CELL GROWTH; CELL INFILTRATION; CELL INTERACTION; CELL PROLIFERATION; CELL TYPE; CELL VIABILITY; CONTROLLED STUDY; DECELLULARIZATION; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; LIVER; LIVER CELL; LIVER CELL CULTURE; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SWINE; TISSUE REGENERATION;

ADULT; ANIMALS; BIOCOMPATIBLE MATERIALS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; EXTRACELLULAR MATRIX; HEPATOCYTES; HUMANS; LIVER; MATERIALS TESTING; POROSITY; SUS SCROFA;

SUS;

EID: 79960834954     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.06.005     Document Type: Article

References (42)

1. Michalopoulos G.K. Liver regeneration after partial hepatectomy: critical analysis of mechanistic dilemmas. Am J Pathol 2010, 176:2-13.
2. Dunn J.C., Yarmush M.L., Koebe H.G., Tompkins R.G. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J 1989, 3:174-177.
3. Sadoshima J., Izumo S. The cellular and molecular response of cardiac myocytes to mechanical stress. Annu Rev Physiol 1997, 59:551-571.
4. Syedain Z.H., Weinberg J.S., Tranquillo R.T. Cyclic distension of fibrin-based tissue constructs: evidence of adaptation during growth of engineered connective tissue. Proc Natl Acad Sci U S A 2008, 105:6537-6542.
5. Funderburgh M.L., Mann M.M., Funderburgh J.L. Keratocyte phenotype is enhanced in the absence of attachment to the substratum. Mol Vis 2008, 14:308-317.
6. Apte U., Gkretsi V., Bowen W.C., Mars W.M., Luo J.H., Donthamsetty S., et al. Enhanced liver regeneration following changes induced by hepatocyte-specific genetic ablation of integrin-linked kinase. Hepatology 2009, 50:844-851.
7. Hammond J.S., Gilbert T.W., Howard D., Zaitoun A., Michalopoulos G., Shakesheff K.M., et al. Scaffolds containing growth factors and extracellular matrix induce hepatocyte proliferation and cell migration in normal and regenerating rat liver. J Hepatol 2011, 54:279-287.
8. Hanley K.P., Oakley F., Sugden S., Wilson D.I., Mann D.A., Hanley N.A. Ectopic SOX9 mediates extracellular matrix deposition characteristic of organ fibrosis. J Biol Chem 2008, 283:14063-14071.
9. Semler E.J., Ranucci C.S., Moghe P.V. Tissue assembly guided via substrate biophysics: applications to hepatocellular engineering. Adv Biochem Eng Biotechnol 2006, 102:1-46.
10. Jun J.I., Lau L.F. Cellular senescence controls fibrosis in wound healing. Aging (Albany NY) 2010, 2:627-631.
11. Tai B.C., Du C., Gao S., Wan A.C., Ying J.Y. The use of a polyelectrolyte fibrous scaffold to deliver differentiated hMSCs to the liver. Biomaterials 2010, 31:48-57.
12. Gilbert T.W., Agrawal V., Gilbert M.R., Povirk K.M., Badylak S.F., Rosen C.A. Liver-derived extracellular matrix as a biologic scaffold for acute vocal fold repair in a canine model. Laryngoscope 2009, 119:1856-1863.
13. Povero D., Busletta C., Novo E., di Bonzo L.V., Cannito S., Paternostro C., et al. Liver fibrosis: a dynamic and potentially reversible process. Histol Histopathol 2010, 25:1075-1091.
14. Mon N.N., Kokuryo T., Hamaguchi M. Inflammation and tumor progression: a lesson from TNF-alpha-dependent FAK signaling in cholangiocarcinoma. Methods Mol Biol 2009, 512:279-293.
15. Yamasaki C., Tateno C., Aratani A., Ohnishi C., Katayama S., Kohashi T., et al. Growth and differentiation of colony-forming human hepatocytes in vitro. J Hepatol 2006, 44:749-757.
16. Everitt E.A., Malik A.B., Hendey B. Fibronectin enhances the migration rate of human neutrophils in vitro. J Leukoc Biol 1996, 60:199-206.
17. Shirahashi H., Wu J., Yamamoto N., Catana A., Wege H., Wager B., et al. Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage. Cell Transplant 2004, 13:197-211.
18. Zhang Y., He Y., Bharadwaj S., Hammam N., Carnagey K., Myers R., et al. Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype. Biomaterials 2009, 30:4021-4028.
19. Liu Y., Bharadwaj S., Lee S.J., Atala A., Zhang Y. Optimization of a natural collagen scaffold to aid cell-matrix penetration for urologic tissue engineering. Biomaterials 2009, 30:3865-3873.
20. Zhang W., Chen X.P., Zhang W.G., Zhang F., Xiang S., Dong H.H., et al. Hepatic non-parenchymal cells and extracellular matrix participate in oval cell-mediated liver regeneration. World J Gastroenterol 2009, 15:552-560.
21. Whitlock P.W., Smith T.L., Poehling G.G., Shilt J.S., Van Dyke M. A naturally derived, cytocompatible, and architecturally optimized scaffold for tendon and ligament regeneration. Biomaterials 2007, 28:4321-4329.
22. Wu S., Liu Y., Bharadwaj S., Atala A., Zhang Y. Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. Biomaterials 2011, 32:1317-1326.
23. Ott H.C., Matthiesen T.S., Goh S.K., Black L.D., Kren S.M., Netoff T.I., et al. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 2008, 14:213-221.
24. Rojkind M., Gatmaitan Z., Mackensen S., Giambrone M.A., Ponce P., Reid L.M. Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes. J Cell Biol 1980, 87:255-263.
25. Baptista P.M., Siddiqui M.M., Lozier G., Rodriguez S.R., Atala A., Soker S. The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 2011, 53:604-617.
26. Kono Y., Yang S., Roberts E.A. Extended primary culture of human hepatocytes in a collagen gel sandwich system. In Vitro Cell Dev Biol Anim 1997, 33:467-472.
27. Peters S.J., Vanhaecke T., Papeleu P., Rogiers V., Haagsman H.P., van Norren K. Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response. Cell Tissue Res 2010, 340:451-457.
28. Bader A., Knop E., Kern A., Boker K., Fruhauf N., Crome O., et al. 3-D coculture of hepatic sinusoidal cells with primary hepatocytes-design of an organotypical model. Exp Cell Res 1996, 226:223-233.
29. Gerlach J.C., Zeilinger K., Spatkowski G., Hentschel F., Schnoy N., Kolbeck S., et al. Large-scale isolation of sinusoidal endothelial cells from pig and human liver. J Surg Res 2001, 100:39-45.
30. Cho C.H., Berthiaume F., Tilles A.W., Yarmush M.L. A new technique for primary hepatocyte expansion in vitro. Biotechnol Bioeng 2008, 101:345-356.
31. Hoshiba T., Cho C.S., Murakawa A., Okahata Y., Akaike T. The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes. Biomaterials 2006, 27:4519-4528.
32. Mingoia R.T., Nabb D.L., Yang C.H., Han X. Primary culture of rat hepatocytes in 96-well plates: effects of extracellular matrix configuration on cytochrome P450 enzyme activity and inducibility, and its application in in vitro cytotoxicity screening. Toxicol in Vitro 2007, 21:165-173.
33. Park K.H., Song S.C. Morphology of spheroidal hepatocytes within injectable, biodegradable, and thermosensitive poly(organophosphazene) hydrogel as cell delivery vehicle. J Biosci Bioeng 2006, 101:238-242.
34. Amin K., Ip C., Sato B., Le T., Green C.E., Tyson C.A., et al. Characterization of ANIT-induced toxicity using precision-cut rat and dog liver slices cultured in a dynamic organ roller system. Toxicol Pathol 2006, 34:776-784.
35. Chang M.L., Sung K.F., Sheen I.S., Lin S.M., Yeh C.T. A liver slice culture-based ex vivo assay to predict the outcome of antiviral therapy for chronic hepatitis C. J Viral Hepat 2009, 16:359-366.
36. Hart A., Mattheyse F.J., Balinsky J.B. An organ culture of postnatal rat liver slices. In Vitro 1983, 19:841-852.
37. McClelland R., Wauthier E., Uronis J., Reid L. Gradients in the liver's extracellular matrix chemistry from periportal to pericentral zones: influence on human hepatic progenitors. Tissue Eng Part A 2008, 14:59-70.
38. Sellaro T.L., Ravindra A.K., Stolz D.B., Badylak S.F. Maintenance of hepatic sinusoidal endothelial cell phenotype in vitro using organ-specific extracellular matrix scaffolds. Tissue Eng 2007, 13:2301-2310.
39. Wang Y., Cui C.B., Yamauchi M., Miguez P., Roach M., Malavarca R., et al. Lineage restriction of human hepatic stem cells to mature fates is made efficient by tissue-specific biomatrix scaffolds. Hepatology 2011, 53:293-305.
40. Baptista P.M., Siddiqui M.M., Lozier G., Rodriguez S.R., Atala A., Soker S. The use of whole organ decellularization for the generation of a vascularized liverorganoid. Hepatology 2011, 53:604-617.
41. Baptista P.M., Orlando G., Mirmalek-Sani S.H., Siddiqui M., Atala A., Soker S. Whole organ decellularization - a tool for bioscaffold fabrication and organ bioengineering. Conf Proc IEEE Eng Med Biol Soc 2009, 6526-6529.
42. Crapo P.M., Gilbert T.W., Badylak S.F. An overview of tissue and whole organ decellularization processes. Biomaterials 2011, 32:3233-3243.