Carotenoids in microalgae

Sub-Cellular Biochemistry

Volumn 79, Issue , 2016, Pages 219-237

  Henríquez, Vitalia ^a   Escobar, Carolina ^a   Galarza, Janeth ^a   Gimpel, Javier ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

Author keywords

Algae; Astaxanthin; Cetocarotenoids; Dunaliella salina; Haematococcus pluvialis; Metabolic engineering

Indexed keywords

CAROTENOID;

BIOSYNTHESIS; BIOTECHNOLOGY; ENERGY TRANSFER; GENETIC ENGINEERING; GENETICS; LIGHT; METABOLISM; MICROALGA; PHOTOSYNTHESIS;

BIOSYNTHETIC PATHWAYS; BIOTECHNOLOGY; CAROTENOIDS; ENERGY TRANSFER; GENETIC ENGINEERING; LIGHT; MICROALGAE; PHOTOSYNTHESIS;

EID: 84983085476     PISSN: 03060225     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-3-319-39126-7_8     Document Type: Article

References (127)

1. Baroli I, Niyogi KK (2000) Molecular genetics of xanthophylls-dependent photoprotection in green algae and plants. Philos Trans R Soc Lond B 355: 1385-1394
2. Barrera D, Gimpel J, Mayfield S (2014) Rapid screening for the robust expression of recombinant proteins in algal plastids. In: Maliga P (ed) Chloroplast biotechnology: methods and protocols. Springer
3. Batista AP, Nunes MC, Fradinho P, Gouveia L, Raymundo A, Franco JM (2011) Novel foods with microalgal ingredients-effect of gel setting conditions on the linear viscoelasticity of Spirulina and Haematococcus gels. J Food Eng 110: 182-189
4. Borowitzka MA, Borowitzka LJ (1987) Vitamins and fine chemicals from micro-algae. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, New York
5. Borowitzka MA (2013) High-value products from microalgae-their development and commercialization. J Appl Phycol 25 (3): 743-756
6. Boussiba S (2000) Carotenogenesis in the green alga Haematococcus pluvialis: cellular physiology and stress response. Physiol Plant 108: 111-117
7. Breithaupt DE (2007) Modern application of xanthophylls in animal feeding-a review. Trends Food Sci Technol 18: 501-506
8. Cadoret JP, Bardor M, Lerouge P, Cabigliera M, Henríquez V, Carlier A (2008) Microalgae as cell factories producing recombinant commercial proteins. Med Sci (Paris) 24: 375-382
9. Cadoret JP, Garnier M, Saint-Jean B (2012) Microalgae, functional genomics and biotech. Adv Bot Res 64: 285-341
10. Camacho JE, González G, Klotz B (2013) Producción de Astaxantina en Haematococcus pluvialis bajo diferentes condiciones de estrés. Scielo 11 (19): 92-104
11. Campenni L, Nobre BP, Santos CA, Oliveira AC, Aires-Barros MR, Palabra MF, Gouveia L (2013) Carotenoid and lipid production by the autotrophic microalga Chlorella protothecoides under nutritional, salinity, and luminosity stress conditions. Appl Microbiol Biotechnol 97: 1383-1393
12. Chacón-Lee TL, González-Mariño GE (2010) Microalgae for “healthy” foods-possibilities and challenges. Compr Rev Food Sci Food 9 (6): 655-675
13. Chana MC, Hoa SH, Lee DJ, Chenc CY, Huang CC, Chang JS (2013) Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNWN. Biochem Eng J 78: 24-31
14. Chen H, Lao YM, Jiang JG (2011) Effects of salinities on the gene expression of a NADC)-dependent glycerol-3-phosphate dehydrogenase in Dunaliella salina. Sci Total Environ 409: 1291-1297
15. Chekanov K, Lobakova E, Selyakh I, Semenova L, Sidorov R, Solovchenko A (2014) Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia). Mar Drugs 12: 4504-4520
16. Chiu CJ, Taylor A (2007) Nutritional antioxidants and age-related cataract and maculopathy. Exp Eye Res 84 (2): 229-245
17. Ciccone MM, Cortese F, Gesualdo M, Carbonara S, Zito A, Ricci G, De Pascalis F, Scicchitano P, Riccioni G (2013) Dietary intake of carotenoids and their antioxidant and anti-inflammatory effects in cardiovascular care. Mediat Inflamm. doi.org/101155/2013/78237
18. Coesel SN, Baumgartner AC, Teles LM, Ramos AA, Henriques NM, Cancela L, Varela JCS (2008a) Nutrient limitation is the main regulatory factor for carotenoid accumulation and for psy and pds steady state transcript levels in Dunaliella salina (Chlorophyta) exposed to high light and salt stress. Mar Biotechnol 10: 602-611
19. Coesel S, Oborník M, Varela J, Falciatore A, Bowler C (2008b) Evolutionary Origins and Functions of the Carotenoid Biosynthetic Pathway in Marine Diatoms. PLo S One 3 (8): e2896
20. Cerón MC, Campos I, Sánchez JF, Acien FG, Molina E, Fernandez-Sevilla JM (2008) Recovery of lutein from microalgae biomass: development of a process for Scenedesmus almeriensis. J Agric Food Chem 56: 11761-11766
21. Cordero BF, Couso I, Leon R, Rodríguez H, Vargas MA (2012) Isolation and characterization of a lycopene epsilon-cyclase gene of Chlorella (Chromochloris) zofingiensis: regulation of the carotenogenic pathway by nitrogen and light. Mar Drugs 10: 2069-2088
22. Cordero BF, Couso I, Leon R, Rodríguez H, Vargas M (2011) Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis. App Microbiol Biotechnol 91: 341-351
23. Cordero BF, Obraztsova I, Martin L, Couso I, Leon R, Vargas MA, Rodriguez H (2010) Isolation and characterization of a lycopene beta-cyclase gene from the astaxanthin-producing green alga Chlorella zofingiensis (Chlorophyta). J Phycol 46: 229-1238
24. Couso I, Vila M, Rodríguez H, Vargas M, Leon R (2011) Overexpression of an Exogenous Phytoene Synthase Gene in the Unicellular Alga Chlamydomonas reinhardtii Leads to an Increase in the Content of Carotenoids. Biotechnol Prog 27 (1): 54-60
25. Cui H, Wang Y, Qin S (2012) Genome wide analysis of carotenoid cleavage dioxygenase in unicellular and filamentous cyanobacteria. Comp Funct Genomics ID 164690: 13
26. Cunningham FX, Gantt E (1998) Genes and enzymes of carotenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 49: 557-583
27. Davies FK, Jinkerson RE, POsewitz MC (2015) Toward a photosynthetic microbial platform for terpenoid engineering. Photosynth Res 123 (3): 265-284
28. Del Campo JA, Garcia-González M, Guerrero MG (2007) Outdoor cuktivation of microalgae for carotenoid production: current state and perspectives. Appl Microbiol Biotechnol 74: 1163-1174
29. Disch A, Schwender J, Müller C, Lichtenthaler HK, Rohmer M (1998) Distribution of the mevalonate and glyceraldehyde phosphate/pyruvate pathways for isoprenoid biosynthesis in unicellular algae and the cyanobacterium Synechocystis PCC 6714. Biochem J 333 (Pt 2): 381-388
30. Dwyer JH, Navab M, Dwyer KM, Hassan K, Sun P, Shircore A, Hama-Levy S, Hough G, Wang X, Drake T, Merz CN, Fogelman AM (2001) Oxygenated carotenoid lutein and progression of early atherosclerosis: the Los Angeles atherosclerosis study. Circulation 103: 2922-2927
31. Emeish S (2012) Production of Natural β-Carotene from Dunaliella Living in the Dead Sea. Jordan J Environ Earth Sci 4 (12): 23-27
32. Frank HA, Cogdell RJ (1996) Carotenoids in photosynthesis. Photochem Photobiol 63 (3): 257-264
33. Dufosse L, Galaup P, Yaron A, Arad SM, Blanc P, Murthy KNC, Ravishankar GA (2005) Microorganisms and microalgae as sources of pigments for food use: a scientific oddity or an industrial reality. Trends Food Sci Technol 16: 389-406
34. Fassett R, Coombes J (2011) Astaxanthin: a potential therapeutic agent in cardiovascular disease. Mar Drugs 9 (3): 447-465
35. Fernández-Sevilla JM, Acién Fernández FG, Molina Grima E (2010) Biotechnological production of lutein and its applications. Appl Microbiol Biotechnol 86: 27-40
36. Fukusaki E-I, Nishikawa T, Kato K, Shinmyo A, Hemmi H, Nishino T, Kobayashi A (2003) Introduction of the archaebacterial geranylgeranyl pyrophosphate synthase gene into Chlamydomonas reinhardtii chloroplast. J Biosci Bioeng 95 (3): 283-287
37. Gao Z, Meng C, Zhang X, Xu D, Miao X, Wang Y, Yang L, Chen L, Ye N (2012) Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis. Enzyme Microb Technol 51: 225-230
38. García-Chavarría M, Lara-Flores M (2013) The use of carotenoid in aquaculture. Res J Fisher Hydrobiol 8 (2): 38-49
39. García-González M, Moreno J, Manzano JC, Florêncio FJ, Guerrero MG (2005) Production of Dunaliella salina biomass rich in 9-cis-β-carotene and lutein in a closed tubular photobioreactor. J Biotechnol 115: 81-90
40. Giannelli L, Yamada L, Katsuda T, Yamaji H (2015) Effects of temperature on the astaxanthin productivity and light harvesting characteristics of the green alga Haematococcus pluvialis. J Biosci Bioeng 119 (3): 345-350
41. Giuliano G, Tavazza R, Diretto G, Beyer P, Taylor MA (2008) Metabolic engineering of carotenoid biosynthesis in plants. Trends Biotechnol 26: 139-145
42. Gong Y, Hu H, Gao Y, Xu X, Gao H (2011) Microalgae as platforms for production of recombinant proteins and valuable compounds: Progress and prospects. J Ind Microbiol Biotechnol 38: 1879-1890
43. Grossman AR, Lohr M, Im CS (2004) Chlamydomonas reinhardtii in the landscape of pigments. Annu Rev Genet 38: 119-173
44. Grünewald K, Hagen C (2000) Extrusion of secondary carotenoid containing vesicles from flagellates of Haematococcus pluvialis (Volvocales; Chlorophyceae). J Appl Bot 74: 141-144
45. Grünewald K, Hirschberg J, Hagen C (2001) Ketocarotenoid biosynthesis outside of plastids in the unicellular green alga Haematococcus pluvialis. J Biol Chem 276: 6023-6029
46. Guedes A, Amaro H, Malcata F (2011) Microalgae as Sources of Carotenoids. Mar Drugs 9: 625-644
47. Guerin M, Huntley ME, Olaizola M (2003) Haematococcus astaxanthin: applications for human health and nutrition. Trends Biotechnol 21: 210-216
48. Han D, Li YT, Hu Q (2013) Astaxanthin in microalgae: pathways, functions and biotechnological implications. Algae 28: 131-147
49. Heider SA, Peters-Wendisch P, Wendisch VF, Beekwilder J, Brautaset T (2014) Metabolic engineering for the microbial production of carotenoids and related products with a focus on the rare C50 carotenoids. Appl Microbiol Biotechnol 98 (10): 4355-4368
50. Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 4: 210-218
51. Ho SH, Chan MC, Liu CC, Chen CY, Lee WL, Lee DJ, Chang JS (2014) Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies. Bioresour Technol 152: 275-282
52. Hong M, Hwang SK, Chang WC, Kim BW, Lee J, Sim SJ (2015) Enhanced autotrophic astaxanthin production from Haematococcus pluvialis under high temperature via heat stress-driven Haber-Weiss reaction. Appl Microbiol Biotechnol doi: 10.1007/s00253-015-6440-6445
53. Houille-Vernes L, Rappaport F, Wollman FA, Alric J, Johnson X (2011) Plastid terminal oxidase 2 (PTOX2) is the major oxidase involved in chlororespiration in Chlamydomonas. Proc Natl Acad Sci U S A 108: 20820-20825
54. Huang JC, Liu J, Li Y, Chen F (2008) Isolation and characterization of the phytoene desaturase gene as a potential selective marker for genetic engineering of the astaxanthin producing green alga Chlorella zofingiensis (Chlorophyta). J Phycol 44: 684-690
55. Huang JC, Wang Y, Sandmann G, Chen F (2006) Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta). Appl Microbiol Biotechnol 71: 473-479
56. Kathiresan S, Arun C, Ravishankar GA, Sarada R (2015) Regulation of astaxanthin and its intermediates through cloning andgenetic transformation of β-carotene ketolase in Haematococcus pluvialis. J Biotechnol 196-197: 33-41
57. Kempinski C, Jiang Z, Bell S, Chappell J (2015) Metabolic engineering of higher plants and algae for isoprenoid production. Adv Biochem Eng Biotechnol. doi: 10.1007/10_2014_290
58. Krishna KB, Mohanty P (1998) J Sci Ind Res (India) 57: 51-63
59. Ladygin VG (2000) Biosynthesis of carotenoids in the chloroplasts of the green alga Haematococcus pluvialis. Russ J Plant Physiol 47: 796-814
60. Lamers P, Janssen M, De Vos R, Bino R, Wijffels R (2008) Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications. Trends Biotechnol 26 (11): 631-638
61. Leon R, Couso I, Fernandez E (2007) Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii. J Biotechnol 130: 143-152
62. Leu S, Boussiba S (2014) Advances in the Production of High-Value Products by Microalgae. Indust Biotechnol 10 (3): 169-183
63. Lemoine Y, Schoefs B (2010) Secondary ketocarotenoid astaxanthin biosynthesis in algae: a multifunctional response to stress. Photosynth Res 106: 155-177
64. Lemoine Y, Rmiki NE, Creach A, Rachidi J, Schoefs B (2008) Cytoplasmic accumulation of astaxanthin by the green alga Haematococcus pluvialis (flotow). In: Schoefs B (ed) Plant Cell Organelles-Selected topics. Research Signpost, Trivandrum, pp 251-284
65. Li M, Zhibing G, Yan C, Chunlei SX (2013) Structure and function characterization of the phytoene desaturase related to the lutein biosynthesis in Chlorella protothecoides CS-41. Mol Biol Rep 40: 3351-3361
66. Li Y, Huang JL, Sandmann G, Chen F (2008) Glucose sensing and the mitochondria alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae). Planta 228: 735-743
67. Li Y, Huang J, Sandmann G, Chen F (2009) High-light and sodium chloride stress differentially regulate the biosynthesis of astaxanthin in Chlorella zofingiensis (Chlorophyceae). J Phycol 45: 635-641
68. Li Y, Sommerfeld M, Chen F, Hu Q (2010) Effect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae). J Appl Phycol 22: 253-263
69. Lichtenthaler HK (1999) The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 50: 47-65
70. Lichtenthaler HK, Rohmer M, Schwender J (1997) Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants. Physiol Plant 101: 643-652
71. Lin JH, Lee DJ, Chang JS (2014) Lutein production from biomass: Marigold flowers versus microalgae. Bioresour Technol 184: 421-428
72. Liu J, Sun Z, Gerken H, Huang J, Jiang Y, Chen F (2014) Genetic engineering of the green alga Chlorella zofingiensis: a modified norflurazon-resistant phytoene desaturase gene as a dominant selectable marker. Appl Microbiol Biotechnol 98: 5069-5079
73. Liu J, Sun Z, Zhong Y, Gerken H, Huang J, Chen F (2013) Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant. J Appl Phycol 25: 1447-1456
74. Liu J, Zhong Y, Sun Z, Huang J, Sandmann G, Chen F (2010) One amino acid substitution in phytoene desaturase makes Chlorella zofingiensis resistant to norflurazon and enhances the biosynthesis of astaxanthin. Planta 232: 61-67
75. Lohr M, Wilhelm C (1999) Algae displaying the diadinoxanthin cycle also possess the violaxanthin cycle. Proc Natl Acad Sci U S A 96: 8784-8789
76. Lohr M, Wilhelm C (2001) Xanthophyll synthesis in diatoms: Quantifications of putative intermediate and comparison of pigment conversion kinetics with rate constants derive form a model. Planta 212: 382-391
77. Lopez J, Fimbres D, Medina LA, Miranda A, Martínez LR, Molina DM (2013) Production of biomass and carotenoids of Dunaliella tertiolecta in nitrogen-limited cultures. Fyton ISSN 0031 9457 (2013) 82: 23-30
78. Lorenz TR, Cysewski GR (2000) Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Trends Biotechnol 18: 160-167
79. Macias-Sanchez MD, Fernández-Sevilla M, Acién-Fernández FG, Cerón-García MC, Molina-Grima E (2010) Supercritical fluid extraction of carotenoids from Scenedesmus almeriensis. Food Chem 123: 928-935
80. Matsuno T (2001) Aquatic animal carotenoids. Fish Sci 67: 771-783. doi: 10.1046/j.1444-2906.2001.00323.x
81. Miki W (1991) Biological functions and activities of animal carotenoids. Pure Appl Chem 63: 141–146
82. Moulin P, Lemoine Y, Schoefs B (2010) Carotenoids and stress in higher plants and algae. In: Pessarakli M (ed) Handbook of plant and crop stress, 3rd edn. Taylor and Francis, New York
83. Mulders K, Lamers PP, Martens DE, Wijffels RH (2014) Phototrophic pigment production with microalgae: biological constraints and opportunities. J Phycol 50: 229-242
84. Naik PS, Chanemougasoundharam A, Paul Khurana SM, Kalloo G (2003) Genetic manipulation of carotenoid pathway in higher plants. Curr Sci 85: 1423-1430
85. Nunes M, Henriques AA, Pinto E, Leal R, Monteiro AC (2013) Carotenogênese em celulas de Haematococcus pluvialis induzidas pelos estresses luminoso e nutricional. Pesq Agropec Bras Brasilia 48 (8): 825-832
86. Olaizola M (2003) Commercial development of microalgal biotechnology: from the test tube to the marketplace. Biomol Eng 20: 459-466
87. Oren A (2005) A hundred years of Dunaliella research: 1905-2005. Saline Syst 1: 2
88. Onogi N, Okuno M, Matsushima-Nishiwaki R, Fukutomi Y, Moriwaki H, Muto Y (1998) Antiproliferative effect of carotenoids on human colon cancer cells without conversion to retinoic acid. Nutr Cancer 32: 20-24
89. Parker MS, Mock T, Armbrust EV (2008) Genomic Insights into Marine Microalgae. Annu Rev Genet 42: 619-645
90. Perusek L, Maeda T (2013) Vitamin A derivatives as treatment options for retinal degenerative diseases. Nutrients 5: 2646-2666
91. Prieto A, Canavate JP, Garcia-Gonzalez M (2011) Assessment of carotenoid production by Dunaliella salina in different culture systems and operation regimes. J Biotechnol 151: 180-185
92. Priyadarshani I, Biswajit R (2012) Commercial and industrial applications of micro algae-a review. J Algal Biomass Utln 3 (4): 89-100
93. Pogson BJ, Mc Donald KA, Truong M, Britton G, Della Penna D (1996) Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants. Plant Cell 8: 1627-1639
94. Pogson BJ, Niyogi KK, Björkman O, Della Penna D (1998) Altered xanthophyll compositions adversely affect chlorophyll accumulation and non-photochemical quenching in Arabidopsis mutants. Proc Natl Acad Sci U S A 95: 13324-13329
95. Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65 (6): 635-648
96. Rabbani S, Beyer P, Von Lintig J, Hugueney P, Kleinig H (1998) Induced beta-carotene synthesis driven by triacylglycerol deposition in the unicellular alga Dunaliella bardawil. Plant Physiol 116: 1239-1248
97. Ramos A, Marques R, Rodrigues M, Henriques N, Baumgartner A, Castilho R, Brenig B, Varela JC (2009) Molecular and functional characterization of a c DNA encoding 4 hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina. J Plant Physiol 166: 968-977
98. Ranga Rao A, Phang SM, Sarada R, Ravishankar GA (2014) Astaxanthin: sources, extraction, stability, biological activities and its commercial applications—a review. Mar Drugs 12: 128-152
99. Rohdich F, Zepeck F, Adam P, Hecht S, Kaiser J (2003) The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: studies on the mechanisms of the reactions catalyzed by Isp G and Isp H protein. Proc Natl Acad Sci U S A 100: 1586-1591
100. Rohmer M (2010) Methylerythritol phosphate pathway. In: Mander L, Lui H-W (eds) Comprehensive natural products II chemistry and biology, vol 1. Elsevier, Oxford
101. Rohmer M (2007) Diversity in isoprene unit biosynthesis: the methylerythritol phosphate pathway in bacteria and plastids. Pure Appl Chem 79: 739-751
102. Sanchez JF, Fernandez JM, Acien FG, Ceron MC, Perez J, Molina E (2008) Biomass and lutein productivity of Scenedesmus almeriensis: influence of irradiance, dilution rate and temperature. Appl Microbiol Biotechnol 79: 719-729
103. Sanchez JF, Fernandez JM, Acien FG, Perez J, Molina E (2007) Influence of culture conditions in the productivity and lutein content of the new strain Scenedesmus almeriensis. Process Biochem 43 (4): 398-405
104. Sanchez-Estudillo L, Freile-Pelegrin Y, Rivera-Madrid R, Robledo D, Narvaez-Zapata JA (2006) Regulation of two photosynthetic pigment-related genes during stress-induced pigment formation in the green alga, Dunaliella salina. Biotechnol Lett 28: 787-791
105. Sandmann G (2009) Evolution of carotene desaturation: the complication of a simple pathway. Arch Biochem Biophys 483: 169-174
106. Sandmann G, Rohmer S, Fraser PD (2006) Understanding carotenoid metabolism as a necessity for genetic engineering of crop plants. Metab Eng 8: 291-302
107. Shi XM, Jiang Y, Chen F (2002) High-yield production of lutein by the green microalgae Chlorella protothecoides in heterotrophic fedbatch culture. Biotechnol Prog 18: 723-727
108. Steinbrenner J, Sandmann G (2006) Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis. Appl Environ Biotech 72 (12): 7477
109. Steinbrenner J, Linden H (2001) Regulation of two carotenoid biosynthesis genes coding for phytoene synthase and carotenoid hydroxylase during stress-induced astaxanthin formation in the green alga Haematococcus pluvialis. Plant Physiol 125: 810-817
110. Sun Z, Cunningham FX, Gantt E (1998) Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular Chlorophyte. Proc Natl Acad Sci U S A 95: 11482-11488
111. Sun N, Wang Y, Li YT, Huang JC, Chen F (2008a) Sugar-based growth, astaxanthin accumulation and carotenogenesis transcription of heterotrophic Chlorella zofingiensis (Chlorophyceae). Process Biochem 43: 1288-1292
112. Sun G, Zhang X, Sui Z, Mao Y (2008b) Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta). Mar Biotechnol (NY) 10 (3): 219-226
113. Sun Z, Liu J, Bi Y, Zhou Z (2014) Microalgae as the Production Platform for Carotenoids In: Liu J, Sun Z, Gerken H (eds) Recent advances in microalgal biotechnology, OMICS Group e Books, Foster City
114. Telfer A (2005) Too much light? How beta-carotene protects the photosystem II reaction centre. Photochem Photobiol Sci 4 (12): 950-956
115. Tran D, Haven J, Qiu W, Polle JE (2009) An update on carotenoid biosynthesis in algae: phylogenetic evidence for the existence of two classes of phytoene synthase. Planta 229: 723-729
116. Vidhyavathi R, Venkatachalam L, Sarada R, Ravishankar GA (2008) Regulation of carotenoid biosynthetic genes expression and carotenoid accumulation in the green alga Haematococcus pluvialis under nutrient stress conditions. J Exp Bot 59: 1409-1418
117. Vila M, Couso I, Leon R (2008) Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase. Process Biochem 43 (10): 1147-1152
118. Wan M, Hou D, Ya L, Fan J, Huang J, Liang S, Wang W, Pan R, Wang J, Li S (2014) The effective photoinduction of Haematococcus pluvialis for accumulating astaxanthin with attached cultivation. Biores Technol 163: 26-32
119. Wang JX, Sommerfeld M, Hu Q (2009) Occurence and environmental stress responses of two plastid terminal oxidases in Haematococcus pluvialis (Chlorophyceae). Planta 230: 191-203
120. Wei D, Chen F, Chen G, Zhang X, Liu L, Zhang H (2008) Enhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stress. Sci China Ser C Life Sci 51: 1088-1093
121. Wong KH (2006) Transgenic Chlamydomonas reinhardtii as an experimental system to study the regulation of carotenoid biosynthesis in green microalgae. Thesis. The University of Hong Kong
122. Wu DY, Wright DA, Wetzel C, Voytas DF, Rodermel S (1999) The immutans variegation locus of Arabidopsis defines a mitochondrial alternative oxidase homolog that functions during early chloroplast biogenesis. Plant Cell 11: 43-55
123. Yaakob Z, Ali E, Zainal A, Mohamad M, Takrif MS (2014) An overview: biomolecules from microalgae for animal feed and aquaculture. J Biol Res-Thessaloniki 21: 6
124. Yan Y, Zhu YH, Jiang JG, Song DL (2005) Cloning and sequence analysis of the phytoene synthase gene from a unicellular chlorophyte, Dunaliella salina. J Agric Food Chem 53: 1466-1469
125. Ye ZW, Jiang JG, Wu GH (2008) Biosynthesis and regulation of carotenoids in Dunaliella: progresses and prospects. Biotechnol Adv 26: 352-360
126. Ye ZW, Jiang JG (2010) Analysis of an Essential Carotenogenic Enzyme: —-Carotene Desaturase from Unicellular Alga Dunaliella salina. J Agric Food Chem 58: 11477-11482
127. Zheng KJ, Wang CG, Xiao M, Chen J, Li JC, Hu ZL (2014) Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas. Sci China Life Sci 57 (10): 1028-1033