Cell-Binding Assays for Determining the Affinity of Protein–Protein Interactions: Technologies and Considerations

Methods in Enzymology

Volumn 580, Issue , 2016, Pages 21-44

  Hunter, S A ^a   Cochran, J R ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

Cell binding assay; Equilibrium; Kd; KinExA; Ligand depletion; SPR; Yeast surface display

Indexed keywords

LIGAND; PROTEIN; PROTEIN BINDING;

BINDING AFFINITY; BINDING ASSAY; CELL ASSAY; CELL SURFACE; COMPETITIVE BINDING ASSAY; CONTROLLED STUDY; INTERMETHOD COMPARISON; KINETIC EXCLUSION ASSAY; MAMMAL CELL; NONHUMAN; PROTEIN BINDING; PROTEIN INTERACTION; SURFACE PLASMON RESONANCE; YEAST CELL; ANIMAL; BINDING COMPETITION; CHEMISTRY; FLOW CYTOMETRY; HUMAN; KINETICS; MAMMAL; PROTEIN PROTEIN INTERACTION; YEAST;

ANIMALS; BINDING, COMPETITIVE; FLOW CYTOMETRY; HUMANS; KINETICS; LIGANDS; MAMMALS; PROTEIN BINDING; PROTEIN INTERACTION MAPS; PROTEINS; YEASTS;

EID: 84979642751     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2016.05.002     Document Type: Chapter

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