Engineering fibronectin-based binding proteins by yeast surface display

Methods in Enzymology

Volumn 523, Issue , 2013, Pages 303-326

  Chen, Tiffany F ^a,b   De Picciotto, Seymour ^a,b   Hackel, Benjamin J ^c   Wittrup, K Dane ^a,b  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c University of Minnesota   (United States)

Author keywords

Alternative scaffolds; Directed evolution; Fibronectin type III domain; Protein engineering; Yeast surface display

Indexed keywords

BINDING PROTEIN; BIOTIN; FIBRONECTIN; PLASMID DNA; STREPTAVIDIN;

ANTIGEN BINDING; ARTICLE; BINDING SITE; CELL DENSITY; CELL GROWTH; CENTRIFUGATION; CULTURE MEDIUM; ELECTROPORATION; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FUNGUS CULTURE; FUNGUS GROWTH; INCUBATION TEMPERATURE; INCUBATION TIME; MUTAGENESIS; PEPTIDE LIBRARY; PLASMID; PRIORITY JOURNAL; PROTEIN ENGINEERING; SCREENING; STAINING; SUPERNATANT; YEAST SURFACE DISPLAY;

EID: 84874091424     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-394292-0.00014-X     Document Type: Chapter

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