A phased strategy to differentiate human CD14+ monocytes into classically and alternatively activated macrophages and dendritic cells

BioTechniques

Volumn 61, Issue 1, 2016, Pages 33-41

  Zarif, Jelani C ^a   Hernandez, James R ^a   Verdone, James E ^a   Campbell, Scott P ^a   Drake, Charles G ^a   Pienta, Kenneth J ^a,b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Johns Hopkins University   (United States)

Author keywords

Cytokine; M1 macrophages; M2 macrophages; Macrophage polarization; Monocyte differentiation; Monocyte derived dendritic cells; Multi nucleated giant cells; Peripheral blood mononuclear cells; Phased strategy

Indexed keywords

B7 ANTIGEN; CD14 ANTIGEN; CD83 ANTIGEN; CD86 ANTIGEN; CELL SURFACE MARKER; INTERLEUKIN 6; MANNOSE; IL6 PROTEIN, HUMAN;

ADAPTIVE IMMUNITY; ARTICLE; CELL DIFFERENTIATION; CELL FUSION; CONTROLLED STUDY; DENDRITIC CELL; ENDOCYTOSIS; FLOW CYTOMETRY; GENE EXPRESSION; GIANT CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; INCUBATION TIME; INNATE IMMUNITY; MACROPHAGE; MALE; MONOCYTE; MRNA EXPRESSION ASSAY; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; POLARIZATION; CELL CULTURE; CYTOLOGY; METABOLISM; PHYSIOLOGY;

ANTIGENS, CD14; CELL DIFFERENTIATION; CELLS, CULTURED; DENDRITIC CELLS; FLOW CYTOMETRY; HUMANS; INTERLEUKIN-6; MACROPHAGES; MONOCYTES;

EID: 84978080131     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114435     Document Type: Article

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