Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors

Biotechnology Journal

Volumn 11, Issue 4, 2016, Pages 473-486

  Rafiq, Qasim A ^a,b   Coopman, Karen ^a   Nienow, Alvin W ^a,c   Hewitt, Christopher J ^a,b  

^a LOUGHBOROUGH UNIVERSITY   (United Kingdom)

^b ASTON UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Bioreactor; Cell therapy bioprocessing; Human mesenchymal stem cell; Microcarrier; Regenerative medicine

Indexed keywords

BIOCONVERSION; BIOREACTORS; BONE; COST EFFECTIVENESS; DIAGNOSIS; FLOWCHARTING; GROWTH KINETICS; KINETICS; MANUFACTURE; METABOLITES; MONOLAYERS; REGENERATIVE MEDICINE; STEM CELLS;

ANCHORAGE-DEPENDENT CELLS; BIOPROCESSING; COST-EFFECTIVE MANUFACTURING; HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS; HUMAN MESENCHYMAL STEM CELLS; LARGE SCALE PRODUCTIONS; MICROCARRIERS; STIRRED TANK BIOREACTORS;

CELL CULTURE;

ARTICLE; BIOREACTOR; BONE MARROW DERIVED MESENCHYMAL STEM CELL; CELL DIFFERENTIATION; CELL EXPANSION; CONTROLLED STUDY; DENSITY; DONOR; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; KINETICS; MESENCHYMAL STEM CELL TRANSPLANTATION; METABOLITE; MICROCARRIER CULTURE; POROSITY; PRIORITY JOURNAL; SURFACE PROPERTY; CELL CULTURE TECHNIQUE; CELL LINE; CELL PROLIFERATION; CYTOLOGY; MESENCHYMAL STROMA CELL; PROCEDURES;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; HUMANS; MESENCHYMAL STROMAL CELLS;

EID: 84977853403     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400862     Document Type: Article

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