Systems for Cell Culture Scale-Up

Animal Cell Culture: Essential Methods

Volumn , Issue , 2011, Pages 297-321

  Halsall, Jennifer ^a   Davis, John M ^b  

^a Eden Biodesign   (United Kingdom)

^b UNIVERSITY OF HERTFORDSHIRE   (United Kingdom)

Author keywords

Adherent cells; Bioreactors (fermenters); Microcarriers; Nutrient and waste product gradients; Suspension cells; Systems for Cell Culture Scale up

Indexed keywords

EID: 84885542333     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470669815.ch10     Document Type: Chapter

References (57)

1. Davis, J.M. (2007) Systems for cell culture scale-up, in Medicines from Animal Cell Culture (eds G.N. Stacey and J.M. Davis), John Wiley & Sons, Ltd, Chichester, UK, pp. 145-171.
2. Hanak, J.A.J. and Davis, J.M. (1995) Hollow fibre bioreactors: The Endotronics Acusyst-Jr. and Maximizer 500, in Cell and Tissue Culture: Laboratory Procedures, Section 28D:3 (eds A. Doyle, J. B. Griffiths and D. G. Newell), John Wiley & Sons, Ltd, Chichester, UK.
3. Davis, J.M. (2007) Hollow-Fiber Cell Culture, in Animal Cell Biotechnology: Methods and Protocols, 2nd edn (ed. R. Poertner) The Humana Press Inc., Totowa, NJ, pp. 337-352.
4. Gey G (1933) An improved technic for massive tissue culture. Am. J. Cancer, 17, 752-756- First description of the use of roller bottles for mammalian cell culture.
5. Griffiths, J.B. (1995) Roller bottle culture, in Cell and Tissue Culture Laboratory Procedures, Section 28A1 (eds A. Doyle, J. B. Griffiths and D. G. Newell), John Wiley & Sons, Ltd, Chichester, UK.
6. Muzzio, F.J., Unger, D.R., Liu M. et al. (1999) Computational and experimental investigation of flow and particle settling in a roller bottle bioreactor. Biotechnol. Bioeng., 63, 185-196.
7. Unger, D.R., Muzzio, F.J., Aunins, J.G. and Singhvi, R. (2000) Computational and experimental investigation of flow and fluid mixing in the roller bottle bioreactor. Biotechnol. Bioeng., 70, 117-130.
8. Tsao, E.I., Bohn, M.A., Ostead, D.R. andMunster, M.J. (1992) Optimization of a roller bottle process for the production of recombinant erythropoietin. Ann. N.Y. Acad. Sci., 665, 127-136.
9. Berson, R.E., Pieczynski, W.J., Svihla, C.K. and Manley, T.R. (2002) Enhanced mixing and mass transfer in a recirculation loop results in high cell densities in a roller bottle reactor. Biotechnol. Prog., 18, 72-77.
10. Knight, E. (1977) Multisurface Glass Roller Bottle for Growth of Animal Cells in Culture. Appl. Environ. Microbiol., 33, 666-669.
11. Griffiths, J.B. (2001) Scale-up of suspension and anchorage-dependent animal cells. Mol. Biotechnol., 17, 223-238.
12. Nardelli, L. and Panina, G.F. (1976) 10-years experience with a 28,800 roller bottle plant for FMD vaccine production. Develop. Biol. Standard., 37, 133-138.
13. Panina, G.F. (1985) Monolayer growth systems: multiple processes, in Animal Cell Biotechnology, vol. 1 (eds R.E. Spier and J.B. Griffiths), Academic Press, London, UK, pp. 211-242.
14. Kunitake, R., Suzuki, A., Ichihashi, H. et al. (1997) Fully-automated roller bottle handling system for large scale culture of mammalian cells. J. Biotechnol. 52, 289-294.
15. Siegl, G., deChastonay, J. and Kronauer, G. (1984) Propagation and assay of hepatitis A virus in vitro. J. Virol. Meth., 9, 53-67.
16. Goetz, R., Kolbeck, R., Lottspeich, F. and Barde, Y.A. (1992) Production and characterization of recombinant mouse neurotrophin-3. Eur. J. Biochem. 204, 745-749.
17. Bishop, N.E., Hugo, D.L., Borovec, S.V. and Anderson, D.A. (1994) Rapid and efficient purification of hepatitis A virus from cell culture. J. Virol. Meth., 47, 203-216.
18. Dickinson, L.A. and Kohwi-Shigematsu, T. (1995) Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol. 15, 456-465.
19. Litjens, T., Bielicki, J., Anson, D.S. et al. (1997) Expression, purification and characterization of recombinant caprine N-acetylglucosamine-6-sulphatase. Biochem. J., 327, 89-94.
20. Nelson, K., Bielicki, J. and Anson, D.S. (1997) Immortalization and characterization of a cell line exhibiting a severe multiple sulphatase deficiency phenotype. Biochem. J., 326 125-130.
21. Ohtaki, T., Ogi, K., Masuda, Y. et al. (1998) Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide. Large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells. J. Biol. Chem. 273, 15464-15473.
22. Lee S-Y, Kim, S.H., Kim, V.N. et al. (1999) Heterologous gene expression in avian cells: potential as a producer of recombinant proteins. J. Biomed. Sci., 6, 8-17.
23. Lay, A.J., Jiang, X.M., Kisker, O. et al. (2000) Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase. Nature 408, 869-873.
24. Suehiro, K., Mizuguchi, J., Nishiyama, K. et al. (2000) Fibrinogen binds to integrin alpha(5)beta(1) via the carboxyl-terminal RGD site of the alpha-chain. J. Biochem. (Tokyo), 128, 705-710.
25. Loewen, N., Bahler, C., Teo,W.L. et al. (2002) Preservation of aqueous outflow facility after second-generation FIV vector-mediated expression of marker genes in anterior segments of human eyes. Inv. Ophth. Vis. Sci., 43, 3686-3690.
26. Hagen, A.J., Aboud, R.A., DePhillips, P.A. et al. (1996) Use of nuclease enzyme in the purification of VAQTA, a hepatitis A vaccine. Biotechnol. Appl. Biochem., 23, 209-215.
27. van Wezel, A.L. (1967) Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature, 216, 64-65.-First description of the use of microcarrier beads for mammalian cell culture.
28. Reuveny, S. (1990) Microcarrier culture systems, in Large-scale Mammalian Cell Culture Technology (ed. E.S. Lubiniecki), Marcel Dekker, New York, USA, pp. 271-341.-A useful evaluation of anchorage-dependent cell propagation systems.
29. Meignier, B., Mougeot, H. and Favre, H. (1980) Foot and mouth disease virus production on microcarrier-grown cells. Dev. Biol. Stand., 46, 249-256.
30. Montagnon, B.J., Fanget, B. and Vincent-Falquet, J.C. (1984) Industrial-scale production of inactivated poliovirus vaccine prepared by culture of Vero cells on microcarrier. Rev. Infect. Dis., 6 (Suppl. 2), S341-S344.
31. Looby, D. and Griffiths, J.B. (1988) Cytotechnology 1, 339-346.-An excellent review of the use of animal cells and appropriate culture systems.
32. GE Healthcare, (2010) http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/DiscontinuedProducts?opendocument&itemid=11002914&newitemid=&text=VSFCYTOPILOTMINIREACTOR. (Accessed November 2010).
33. Nie, Y., BergendahI, V., Hei, D.J. et al. (2009) Scalable culture and cryopreservation of human embryonic stem cells on microcarriers. Biotechnol Prog., 25, 20-31.
34. Giard, D.J., Thilly, W.G., Wang, D.I.C. and Levine, D.W. (1977) Virus production with a newly developed microcarrier system. Appl. Env. Microbiol., 34, 668-672.
35. Cherry, R.S. and Papoutsakis, E.T. (1988) Physical mechanisms of cell damage in microcarrier cell culture bioreactors. Biotechnol. Bioeng., 32, 1001-1014.-A useful discussion of the mechanisms of cell damage during microcarrier culture.
36. Croughan, M.S., Hamel, J-F. andWang, D.I.C. (1987) Hydrodynamic effects on animal cells grown in microcarrier cultures. Biotechnol. Bioeng., 29, 130-141.-A detailed explanation of the hydrodynamic effects exerted on animal cells grown in microcarrier cultures.
37. Croughan, M.S., Hamel J-F. and Wang, D.I.C. (1988) Effects of microcarrier concentration in animal cell culture. Biotechnol. Bioeng., 32, 975-982.
38. van Wezel, A.L. (1985) Monolayer growth systems: homogeneous unit processes, in Animal Cell Biotechnology, vol. 1 (eds R. E. Spier and J. B. Griffiths), Academic Press, London, UK, pp. 265-282.
39. Levine, D.W., Wang, D.I.C. and Thilly, W.G. (1977) Optimizing parameters for growth of anchorage-dependent mammalian cells in microcarrier culture, in Cell Culture and its Application (eds R. T. Acton and J. D. Lynn), Academic Press, New York, NY, pp. 191-216.
40. Levine, D.W., Wang, D.I.C. and Thilly, W.G. (1979) Optimization of growth surface parameters in microcarrier cell culture. Biotechnol. Bioeng., 21, 821-845.
41. Bailey, J.E. and Ollis, D. (1986) Biochemical Engineering Fundamentals, 2nd edn, McGraw-Hill, Columbus, OH.
42. van't Riet, K. and Tramper, J. (1991) Basic Bioreactor Design, Marcel Dekker, Abingdon, UK.
43. Doran, P. (1995) Bioprocess Engineering Principles, Elsevier, Amsterdam, the Netherlands.
44. Asenjo, J. and Merchuk, J. (1994) Bioreactor System Design,Marcel Dekker, Abingdon, UK.
45. Stanbury, P.F., Whitaker, A. and Hall, S. (1999) Principles of Fermentation Technology, 2nd edn, Elsevier, London, UK.
46. Nielsen, J., Villadsen, J. and Liden, G. (2002) Bioreaction Engineering Principles, 2nd edn, Springer, New York, NY.
47. Hasler, L., Butz, R. and Graf, P. (2006) Transparency, Form and Function. Fermenter Manufacturing- Art, Three Point Publishing House, CH-8636 Wald, Switzerland.
48. Cino, J. and Frey, S. (1996) 20 Tips for purchasing research fermentors and bioreactors: a practical guide for researchers, Part 1. BioPharm, 9, 52-56.-Along with reference 49, a useful guide to purchasing bioreactors.
49. Cino, J. and Frey, S. (1997) 20 tips for purchasing research fermentors and bioreactors: a practical guide for researchers, Part 2. BioPharm, 10, 42-48.
50. Lim, A.C., Washbrook, J., Titchener-Hooker, N.J. and Farid, S.S. (2006) A computer-aided approach to compare the production economics of fed-batch and perfusion culture under uncertainty. Biotechnol. Bioeng., 93, 687-697.
51. Schoof, D.D., Gramolini, B.A., Davidson, D.L. et al. (1988) Adoptive immunotherapy of human cancer using low-dose recombinant interleukin 2 and lymphokine-activated killer cells. Cancer Res., 48, 5007-5010.
52. Singh, V. (1999) Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology, 30, 140-158.-Description of the development of a disposable bioreactor for cell culture that employed wave-induced agitation.
53. Fries, S., Glazomitsky, K.,Woods, A. et al. (2005) Evaluation of disposable bioreactors: rapid production of recombinant proteins by several animal cell lines. BioProcess Int, October, 36-44.-A review of suitable bioreactor types for animal cells.
54. Namdev, P. and Lio, P. (2000) Assessing a disposable bioreactor for attachment-dependent cell cultures. BioPharm, 13, 44-50.
55. Pierce, L.N. and Shabram, P.W. (2004) Scalability of a disposable bioreactor from 12-500 L. BioProc. J., 3, 1-6.
56. WAVE Bioreactor Systems. www.wavebiotech.com (Accessed September 2010).
57. Handa-Corrigan, A. (1990) Oxygenating animal cell cultures: the remaining problems, in Animal Cell Biotechnology, vol. 4 (eds R.E. Spier and J.B. Griffiths), Academic Press, pp. 123-132.