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Volumn 85, Issue , 2014, Pages 79-88

A potentially scalable method for the harvesting of hMSCs from microcarriers

Author keywords

Agitation; Cell harvest; Downstream processing; Human mesenchymal stem cells; Large scale processing; Microcarriers

Indexed keywords

AGITATION; DOWNSTREAM-PROCESSING; HUMAN MESENCHYMAL STEM CELLS; LARGE-SCALE PROCESSING; MICROCARRIERS;

EID: 84896844378     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2014.02.005     Document Type: Article
Times cited : (122)

References (51)
  • 1
    • 84879840462 scopus 로고    scopus 로고
    • Culture of human mesenchymal stem cells on microcarriers in a 5l stirred-tank bioreactor
    • Rafiq Q.A., Brosnan K.M., Coopman K., Nienow A.W., Hewitt C.J. Culture of human mesenchymal stem cells on microcarriers in a 5l stirred-tank bioreactor. Biotechnol. Lett. 2013, 35:1233-1245.
    • (2013) Biotechnol. Lett. , vol.35 , pp. 1233-1245
    • Rafiq, Q.A.1    Brosnan, K.M.2    Coopman, K.3    Nienow, A.W.4    Hewitt, C.J.5
  • 2
    • 83655212727 scopus 로고    scopus 로고
    • Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask
    • Want A.J., Nienow A.W., Hewitt C.J., Coopman K. Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask. Regen. Med. 2012, 7:71-84.
    • (2012) Regen. Med. , vol.7 , pp. 71-84
    • Want, A.J.1    Nienow, A.W.2    Hewitt, C.J.3    Coopman, K.4
  • 7
    • 70350567179 scopus 로고    scopus 로고
    • Ex vitro expansion of human placenta-derived mesenchymal stem cells in stirred bioreactor
    • Yu Y., Li K., Bao C., Liu T., Jin Y., Ren H., Yun W. Ex vitro expansion of human placenta-derived mesenchymal stem cells in stirred bioreactor. Appl. Biochem. Biotechnol. 2009, 159:110-118.
    • (2009) Appl. Biochem. Biotechnol. , vol.159 , pp. 110-118
    • Yu, Y.1    Li, K.2    Bao, C.3    Liu, T.4    Jin, Y.5    Ren, H.6    Yun, W.7
  • 8
    • 78649966348 scopus 로고    scopus 로고
    • Suspension culture of mammalian cells using thermosensitive microcarrier that allows cell detachment without proteolytic enzyme treatment
    • Yang H.S., Jeon O., Bhang S.H., Lee S.H., Kim B.S. Suspension culture of mammalian cells using thermosensitive microcarrier that allows cell detachment without proteolytic enzyme treatment. Cell Transplant. 2010, 19:1123-1132.
    • (2010) Cell Transplant. , vol.19 , pp. 1123-1132
    • Yang, H.S.1    Jeon, O.2    Bhang, S.H.3    Lee, S.H.4    Kim, B.S.5
  • 9
    • 77950457742 scopus 로고    scopus 로고
    • Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system
    • Eibes G., dos Santos F., Andrade P.Z., Boura J.S., Abecasis M.M., da Silva C.L., Cabral J.M. Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system. J. Biotechnol. 2010, 146:194-197.
    • (2010) J. Biotechnol. , vol.146 , pp. 194-197
    • Eibes, G.1    dos Santos, F.2    Andrade, P.Z.3    Boura, J.S.4    Abecasis, M.M.5    da Silva, C.L.6    Cabral, J.M.7
  • 10
    • 60549096066 scopus 로고    scopus 로고
    • Ear mesenchymal stem cells: an efficient adult multipotent cell population fit for rapid and scalable expansion
    • Sart S., Schneider Y.-J., Agathos S.N. Ear mesenchymal stem cells: an efficient adult multipotent cell population fit for rapid and scalable expansion. J. Biotechnol. 2009, 139:291-299.
    • (2009) J. Biotechnol. , vol.139 , pp. 291-299
    • Sart, S.1    Schneider, Y.-J.2    Agathos, S.N.3
  • 11
    • 84895520546 scopus 로고    scopus 로고
    • Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension culture
    • Yuan Y., Kallos M.S., Hunter C., Sen A. Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension culture. J. Tissue Eng. Regen. Med. 2012, 1515. 10.1002/term.1515.
    • (2012) J. Tissue Eng. Regen. Med. , pp. 1515
    • Yuan, Y.1    Kallos, M.S.2    Hunter, C.3    Sen, A.4
  • 12
    • 77956232755 scopus 로고    scopus 로고
    • Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential
    • Sun L.Y., Hsieh D.K., Syu W.S., Li Y.S., Chiu H.T., Chiou T.W. Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential. Cell Prolif. 2010, 43:445-456.
    • (2010) Cell Prolif. , vol.43 , pp. 445-456
    • Sun, L.Y.1    Hsieh, D.K.2    Syu, W.S.3    Li, Y.S.4    Chiu, H.T.5    Chiou, T.W.6
  • 14
    • 84991951117 scopus 로고    scopus 로고
    • Scale-up of human mesenchymal stem cells on microcarriers in suspension in a single-use bioreactor
    • Kehoe D., Schnitzler A., Simler J., DiLeo A., Ball A. Scale-up of human mesenchymal stem cells on microcarriers in suspension in a single-use bioreactor. Bioprocess Int. 2012, 24:28-38.
    • (2012) Bioprocess Int. , vol.24 , pp. 28-38
    • Kehoe, D.1    Schnitzler, A.2    Simler, J.3    DiLeo, A.4    Ball, A.5
  • 15
    • 84886786622 scopus 로고    scopus 로고
    • Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: achievements and future direction
    • Chen A.K.-L., Reuveny S., Oh S.K.W. Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: achievements and future direction. Biotechnol. Adv. 2013, 31:1032-1046.
    • (2013) Biotechnol. Adv. , vol.31 , pp. 1032-1046
    • Chen, A.K.-L.1    Reuveny, S.2    Oh, S.K.W.3
  • 18
    • 45149091594 scopus 로고    scopus 로고
    • Culturing of human mesenchymal stem cells as three-dimensional aggregates induces functional expression of CXCR4 that regulates adhesion to endothelial cells
    • Potapova I.A., Brink P.R., Cohen I.S., Doronin S.V. Culturing of human mesenchymal stem cells as three-dimensional aggregates induces functional expression of CXCR4 that regulates adhesion to endothelial cells. J. Biol. Chem. 2008, 283:13100-13107.
    • (2008) J. Biol. Chem. , vol.283 , pp. 13100-13107
    • Potapova, I.A.1    Brink, P.R.2    Cohen, I.S.3    Doronin, S.V.4
  • 20
    • 84897549019 scopus 로고    scopus 로고
    • Comparison of enzymatic and non-enzymatic means of dissociating adherent monolayers of mesenchymal stem cells
    • Heng B.C., Cowan C.M., Basu S. Comparison of enzymatic and non-enzymatic means of dissociating adherent monolayers of mesenchymal stem cells. Biol. Proced. Online 2009, 11:161-169.
    • (2009) Biol. Proced. Online , vol.11 , pp. 161-169
    • Heng, B.C.1    Cowan, C.M.2    Basu, S.3
  • 21
    • 85079920548 scopus 로고    scopus 로고
    • Developing a standardised manufacturing process for the clinical-scale production of human mesenchymal stem cells
    • Q.A. Rafiq, Developing a standardised manufacturing process for the clinical-scale production of human mesenchymal stem cells, PhD Thesis, Loughborough University, UK.
    • PhD Thesis, Loughborough University, UK.
    • Rafiq, Q.A.1
  • 22
    • 85080022592 scopus 로고    scopus 로고
    • SoloHill Engineering Inc., Protocol for small scale microcarrier culture, Report MCP-001A
    • SoloHill Engineering Inc., Protocol for small scale microcarrier culture, Report MCP-001A, 2010. http://www.solohill.com/.
    • (2010)
  • 23
    • 33747343749 scopus 로고    scopus 로고
    • Reactor engineering in large scale animal cell culture
    • Nienow A.W. Reactor engineering in large scale animal cell culture. Cytotechnology 2006, 50:9-33.
    • (2006) Cytotechnology , vol.50 , pp. 9-33
    • Nienow, A.W.1
  • 24
    • 38949132055 scopus 로고    scopus 로고
    • Microcarrier cell culture technology
    • Humana Press
    • Blüml G. Microcarrier cell culture technology. Animal Cell Biotechnology 2007, 24:149-178. Humana Press.
    • (2007) Animal Cell Biotechnology , vol.24 , pp. 149-178
    • Blüml, G.1
  • 25
    • 84888312711 scopus 로고    scopus 로고
    • Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture
    • Nienow A.W., Scott W.H., Hewitt C.J., Thomas C.R., Lewis G., Amanullah A., Kiss R., Meier S.J. Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture. Chem. Eng. Res. Des. 2013, 91:2265-2274.
    • (2013) Chem. Eng. Res. Des. , vol.91 , pp. 2265-2274
    • Nienow, A.W.1    Scott, W.H.2    Hewitt, C.J.3    Thomas, C.R.4    Lewis, G.5    Amanullah, A.6    Kiss, R.7    Meier, S.J.8
  • 26
    • 84877337442 scopus 로고    scopus 로고
    • The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4
    • Nienow A.W., Rielly C.D., Brosnan K., Bargh N., Lee K., Coopman K., Hewitt C.J. The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4. Biochem. Eng. J. 2013, 76:25-36.
    • (2013) Biochem. Eng. J. , vol.76 , pp. 25-36
    • Nienow, A.W.1    Rielly, C.D.2    Brosnan, K.3    Bargh, N.4    Lee, K.5    Coopman, K.6    Hewitt, C.J.7
  • 27
    • 84988099027 scopus 로고
    • Physical mechanisms of cell damage in microcarrier cell culture bioreactors
    • Cherry R.S., Papoutsakis E.T. Physical mechanisms of cell damage in microcarrier cell culture bioreactors. Biotechnol. Bioeng. 1988, 32:1001-1014.
    • (1988) Biotechnol. Bioeng. , vol.32 , pp. 1001-1014
    • Cherry, R.S.1    Papoutsakis, E.T.2
  • 28
    • 79961190246 scopus 로고    scopus 로고
    • Scale-up stirred tank reactors
    • John Wiley & Sons Inc.
    • Nienow A.W. Scale-up stirred tank reactors. Encyclopedia of Industrial Biotechnology 2010, vol. 7:4328-4341. John Wiley & Sons Inc.
    • (2010) Encyclopedia of Industrial Biotechnology , vol.7 , pp. 4328-4341
    • Nienow, A.W.1
  • 29
    • 0023092907 scopus 로고
    • Hydrodynamic effects on animal cells grown in microcarrier cultures
    • Croughan M.S., Hamel J.F., Wang D.I. Hydrodynamic effects on animal cells grown in microcarrier cultures. Biotechnol. Bioeng. 1987, 29:130-141.
    • (1987) Biotechnol. Bioeng. , vol.29 , pp. 130-141
    • Croughan, M.S.1    Hamel, J.F.2    Wang, D.I.3
  • 30
    • 84988099707 scopus 로고
    • Effects of microcarrier concentration in animal cell culture
    • Croughan M.S., Hamel J.F., Wang D.I. Effects of microcarrier concentration in animal cell culture. Biotechnol. Bioeng. 1988, 32:975-982.
    • (1988) Biotechnol. Bioeng. , vol.32 , pp. 975-982
    • Croughan, M.S.1    Hamel, J.F.2    Wang, D.I.3
  • 31
    • 0031648103 scopus 로고    scopus 로고
    • Hydrodynamics of stirred bioreactors
    • Nienow A.W. Hydrodynamics of stirred bioreactors. Appl. Mech. Rev. 1998, 51:3-32.
    • (1998) Appl. Mech. Rev. , vol.51 , pp. 3-32
    • Nienow, A.W.1
  • 32
    • 58149218117 scopus 로고    scopus 로고
    • Use of angle resolved PIV to estimate local specific energy dissipation rates for up- and down-pumping pitched blade agitators in a stirred tank
    • Gabriele A., Nienow A.W., Simmons M.J.H. Use of angle resolved PIV to estimate local specific energy dissipation rates for up- and down-pumping pitched blade agitators in a stirred tank. Chem. Eng. Sci. 2009, 64:126-143.
    • (2009) Chem. Eng. Sci. , vol.64 , pp. 126-143
    • Gabriele, A.1    Nienow, A.W.2    Simmons, M.J.H.3
  • 33
  • 34
    • 0030243651 scopus 로고    scopus 로고
    • Impact of tank geometry on the maximum turbulence energy dissipation rate for impellers
    • Zhou G., Kresta S.M. Impact of tank geometry on the maximum turbulence energy dissipation rate for impellers. AIChE J. 1996, 42:2476-2490.
    • (1996) AIChE J. , vol.42 , pp. 2476-2490
    • Zhou, G.1    Kresta, S.M.2
  • 35
    • 34548497149 scopus 로고    scopus 로고
    • The scale-up of microbial batch and fed-batch fermentation processes
    • Hewitt C.J., Nienow A.W. The scale-up of microbial batch and fed-batch fermentation processes. Adv. Appl. Microbiol. 2007, 62:105-135.
    • (2007) Adv. Appl. Microbiol. , vol.62 , pp. 105-135
    • Hewitt, C.J.1    Nienow, A.W.2
  • 36
    • 0021866189 scopus 로고
    • Drop sizes of emulsions related to turbulent energy dissipation rates
    • Davies J.T. Drop sizes of emulsions related to turbulent energy dissipation rates. Chem. Eng. Sci. 1985, 40:839-842.
    • (1985) Chem. Eng. Sci. , vol.40 , pp. 839-842
    • Davies, J.T.1
  • 37
  • 38
    • 0032487129 scopus 로고    scopus 로고
    • The use of flow cytometry to study the impact of fluid mechanical stress on Escherichia coli W3110 during continuous cultivation in an agitated bioreactor
    • Hewitt C.J., Boon L.A., McFarlane C.M., Nienow A.W. The use of flow cytometry to study the impact of fluid mechanical stress on Escherichia coli W3110 during continuous cultivation in an agitated bioreactor. Biotechnol. Bioeng. 1998, 59:612-620.
    • (1998) Biotechnol. Bioeng. , vol.59 , pp. 612-620
    • Hewitt, C.J.1    Boon, L.A.2    McFarlane, C.M.3    Nienow, A.W.4
  • 39
    • 0038522517 scopus 로고    scopus 로고
    • The impact of fluid mechanical stress on Saccharomyces cerevisiae cells during continuous cultivation in an agitated, aerated bioreactor; its implication for mixing in the brewing process and aerobic fermentations
    • Boswell C.D., Nienow A.W., Gill N.K., Kocharunchitt S., Hewitt C.J. The impact of fluid mechanical stress on Saccharomyces cerevisiae cells during continuous cultivation in an agitated, aerated bioreactor; its implication for mixing in the brewing process and aerobic fermentations. Food Bioprod. Process. 2003, 81:23-32.
    • (2003) Food Bioprod. Process. , vol.81 , pp. 23-32
    • Boswell, C.D.1    Nienow, A.W.2    Gill, N.K.3    Kocharunchitt, S.4    Hewitt, C.J.5
  • 40
    • 0004294969 scopus 로고    scopus 로고
    • Butterworth-Heinemann, Oxford, UK
    • Mullin J.W. Crystallization 2001, Butterworth-Heinemann, Oxford, UK. 4th ed.
    • (2001) Crystallization
    • Mullin, J.W.1
  • 41
    • 0017924196 scopus 로고
    • Particle abrasion at high solids concentration in stirred vessels
    • Nienow A.W., Conti R. Particle abrasion at high solids concentration in stirred vessels. Chem. Eng. Sci. 1978, 33:1077-1086.
    • (1978) Chem. Eng. Sci. , vol.33 , pp. 1077-1086
    • Nienow, A.W.1    Conti, R.2
  • 42
    • 0009714955 scopus 로고
    • The effect of agitation and scale-up on crystal growth rates and secondary nucleation
    • Nienow A.W. The effect of agitation and scale-up on crystal growth rates and secondary nucleation. Trans. Inst. Chem. Eng. 1976, 54:205-207.
    • (1976) Trans. Inst. Chem. Eng. , vol.54 , pp. 205-207
    • Nienow, A.W.1
  • 43
    • 5444249124 scopus 로고    scopus 로고
    • Bioprocess equipment: characterization of energy dissipation rate and its potential to damage cells
    • Mollet M., Ma N., Zhao Y., Brodkey R., Taticek R., Chalmers J.J. Bioprocess equipment: characterization of energy dissipation rate and its potential to damage cells. Biotechnol. Prog. 2004, 20:1437-1448.
    • (2004) Biotechnol. Prog. , vol.20 , pp. 1437-1448
    • Mollet, M.1    Ma, N.2    Zhao, Y.3    Brodkey, R.4    Taticek, R.5    Chalmers, J.J.6
  • 49
    • 60249089699 scopus 로고    scopus 로고
    • Mixing studies in a model aerated bioreactor equipped with an up- or a down-pumping 'Elephant Ear' agitator: power, hold-up and aerated flow field measurements, Chem
    • Zhu H., Nienow A.W., Bujalski W., Simmons M.J.H. Mixing studies in a model aerated bioreactor equipped with an up- or a down-pumping 'Elephant Ear' agitator: power, hold-up and aerated flow field measurements, Chem. Eng. Res. Des. 2009, 87:307-317.
    • (2009) Eng. Res. Des. , vol.87 , pp. 307-317
    • Zhu, H.1    Nienow, A.W.2    Bujalski, W.3    Simmons, M.J.H.4
  • 50
    • 0030239355 scopus 로고    scopus 로고
    • Particle suspension in the turbulent regime: the effect of impeller type and impeller/vessel configuration
    • Ibrahim S., Nienow A.W. Particle suspension in the turbulent regime: the effect of impeller type and impeller/vessel configuration. Chem. Eng. Res. Des. 1996, 74:679-688.
    • (1996) Chem. Eng. Res. Des. , vol.74 , pp. 679-688
    • Ibrahim, S.1    Nienow, A.W.2
  • 51
    • 84872198285 scopus 로고    scopus 로고
    • Evaluation of single-use fluidized bed centrifuge system for mammalian cell harvesting
    • Ko H., Bhatia R. Evaluation of single-use fluidized bed centrifuge system for mammalian cell harvesting. BioPharm. Int. 2012, 25:34-40.
    • (2012) BioPharm. Int. , vol.25 , pp. 34-40
    • Ko, H.1    Bhatia, R.2


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