A microcarrier-based cultivation system for expansion of primary mesenchymal stem cells

Biotechnology Progress

Volumn 23, Issue 1, 2007, Pages 187-193

  Frauenschuh, Simone ^a   Reichmann, Elisabeth ^a,b   Ibold, Yvonne ^a,b   Goetz, Peter M ^a   Sittinger, Michael ^b   Ringe, Jochen ^b  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; HISTOLOGY; POISSON DISTRIBUTION; SCANNING ELECTRON MICROSCOPY; THREE DIMENSIONAL; TISSUE; TWO DIMENSIONAL;

HISTOCHEMISTRY; MICROCARRIER CULTURES; MICROCARRIERS; PRIMARY MESENCHYMAL STEM CELLS (MSC);

CELL CULTURE;

ANIMAL; BIOREACTOR; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CONFERENCE PAPER; CULTURE TECHNIQUE; CYTOLOGY; EQUIPMENT; EQUIPMENT DESIGN; INSTRUMENTATION; MESENCHYMAL STEM CELL; METHODOLOGY; PHYSIOLOGY; SWINE; TISSUE ENGINEERING;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; MESENCHYMAL STEM CELLS; SWINE; TISSUE ENGINEERING;

SUS;

EID: 33847103756     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp060155w     Document Type: Conference Paper

References (39)

1. Baksh, D.; Davies, J. E.; Zandstra, P. W. Adult human bone marrow-derived mesenchymal progenitor cells are capable of adhesion-independent survival and expansion. Exp. Hematol. 2003, 31, 723-732.
2. Barry, F. P.; Murphy, J. M. Mesenchymal stem cells: clinical applications and biological characterization. Int. J. Biochem. Cell. Biol. 2004, 36, 568-584.
3. Bonassar, L. J.; Vacanti, C. A. Tissue engineering: the first decade and beyond. J. Cell. Biochem. Suppl. 1998, 30-31, 297-303.
4. Bruder, S. P.; Jaiswal, N.; Haynesworth, S. E. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J. Cell. Biochem. 1997, 64, 278-294.
5. Caplan, A. I. The mesengenic process. Clin. Plast. Surg. 1994, 21, 429-435.
6. Cheng, L.; Qasba, P.; Vanguri, P.; Thiede, M. A. Human mesenchymal stem cells support megakaryocyte and pro-platelet formation from CD34(+) hematopoietic progenitor cells. J. Cell. Physiol. 2000, 184, 58-69.
7. Chun, K. W.; Yoo, H. S.; Yoon, J. J.; Park, T. G. Biodegradable PLGA microcarriers for injectable delivery of chondrocytes: effect of surface modification on cell attachment and function. Biotechnol. Prog. 2004, 20, 1797-1801.
8. Colter, D. C.; Sekiya, I.; Prockop, D. J. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 7841-7845.
9. Conget, P. A.; Minguell, J. J. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J. Cell. Physiol. 1999, 181, 67-73.
10. Digirolamo, C. M.; Stokes, D.; Colter, D.; Phinney, D. G.; Class, R.; Prockop, D. J. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br. J. Haematol. 1999, 107, 275-281.
11. Durrschmid, M.; Landauer, K.; Simic, G.; Blumel, G.; Doblhoff-Dier. Scalable inocculation strategies for microcarriere based animal cell bioprocesses. Biotechnol. Bioeng. 2003, 83, 681-686.
12. Frondoza, C.; Sohrabi, A.; Hungerford, D. Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture. Biomaterials 1996, 17, 879-888.
13. Hong, Y.; Gao, C.; Xie, Y.; Gong, Y.; Shen, J. Collagen-coated polylactide microspheres as chondrocyte microcarriers. Biomaterials 2005, 26, 6305-6313.
14. Hu, W. S.; Meier, J.; Wang, D. I. C. A mechanistic analysis of the inoculum requirement of the cultivation of mamalian cells on microcarriers. Biotechnol. Bioeng. 1985, 27, 585-595.
15. Jaiswal, N.; Haynesworth, S. E.; Caplan, A. I.; Bruder, S. P. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 1997, 64, 295-312.
16. Johnstone, B.; Hering, T. M.; Caplan, A. I.; Goldberg, V. M.; Yoo, J. U. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp. Cell. Res. 1998, 238, 265-272.
17. Kassem, M.; Kristiansen, M.; Abdallah, B. M. Mesenchymal stem cells: cell biology and potential use in therapy. Basic Clin. Pharmacol. Toxicol. 2004, 95, 209-214.
18. Kuo, Y. C.; Chung, C. Y. Application of bovine pituitary extract and polyglycolide/poly(lactide-co-glycolide) scaffold to the cultivation of bovine knee chondrocytes. Biotechnol. Prog. 2005, 21, 1708-1715.
19. Lennon, D. P.; Haynesworth, S. E.; Bruder, S. P.; Jailswal, N.; Caplan, A. I. Human and and animal mesenchymal progenitor cells from bone marrow. Identification of serum for optimal selection and proliferation. In Vitro Dev. Biol. 1996, 32, 602-611.
20. Levine, D. W.; Wong, J. S.; Wang, D. I.; Thilly, W. G. Microcarrier cell culture: new methods for research-scale application. Somatic Cell. Genet 1977, 3, 149-155.
21. Mackay, A. M.; Beck, S. C.; Murphy, J. M.; Barry, F. P.; Chichester, C. O.; Pittenger, M. F. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng. 1998, 4, 415-428.
22. Majumdar, M. K.; Thiede, M. A.; Mosca, J. D.; Moorman, M.; Gerson, S. L. Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J. Cell. Physiol. 1998, 176, 57-66.
23. Malda, J.; Kreijveld, E.; Temenoff, J. S.; van Blitterswijk, C. A.; Riesle, J. Expansion of human nasal chondrocytes on macroporous microcarriers enhances redifferentiation. Biomaterials 2003, 24, 5153-5161.
24. Malda, J.; van Blitterswijk, C. A.; Grojec, M.; Martens, D. E.; Tramper, J.; Riesle, J. Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue Eng. 2003, 9, 939-948.
25. Melero-Martin, J. M.; Dowling, M. A.; Smith, M.; Al-Rubeai, M. Expansion of chondroprogenitor cells on macroporous micro-carriers as an alternative to conventional monolayer systems. Biomaterials 2006, 27, 2970-2979.
26. Minuth, W. W.; Kloth, S.; Aigner, J.; Sittinger, M.; Rockl, W. Approach to an organo-typical environment for cultured cells and tissues. Biotechniques 1996, 20, 498-501.
27. Muraglia, A., Cancedda, R., Quarto, R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J. Cell Sci. 2000, 113, 1161-1166.
28. Ng, Y. C.; Berry, J. M.; Butler, M. Optimization of physical parameters for cell attachment on macroporous microcarriers. Biotechnol. Bioeng. 1996, 50, 627.
29. Ohya, Y.; Matsunami, H.; Yamabe, E.; Ouchi, T. Cell attachment and growth on films prepared from poly(depsipeptide-co-lactide) having various functional groups. J. Biomed. Mater. Res. A. 2003, 65, 79-88.
30. Otto, W. R.; Rao, J. Tomorrow's skeleton staff: mesenchymal stem cells and the repair of bone and cartilage. Cell. Prolif. 2004, 37, 97-110.
31. Pittenger, M. F.; Mackay, A. M.; Beck, S. C.; Jaiswal, R. K.; Douglas, R.; Mosca, J. D.; Moorman, M. A.; Simonetti, D. W.; Craig, S.; Marshak, D. R. Multilineage potential of adult human mesenchymal stem cells. Science 1999, 284, 143-147.
32. Ringe, J.; Kaps, C.; Burmester, G. R.; Sittinger, M. Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften 2002, 89, 338-351.
33. Ringe, J.; Kaps, C.; Schmitt, B.; Buscher, K.; Bartel, J.; Smolian, H.; Schultz, O.; Burmester, G. R.; Haupl, T.; Sittinger, M. Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages. Cell Tissue Res. 2002, 307, 321-327.
34. Ryan, J. M.; Barry, F. P.; Murphy, J. M.; Mahon, B. P. Mesenchymal stem cells avoid allogeneic rejection. J. Inflammation (London) 2005, 2, 8.
35. Sekiya, I.; Larson, B. L.; Vuoristo, J. T.; Cui, J. G.; Prockop, D. J. Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs). J. Bone Miner. Res. 2004, 19, 256-264.
36. Sittinger, M.; Hutmacher, D. W.; Risbud, M. V. Current strategies for cell delivery in cartilage and bone regeneration. Curr. Opin. Biotechnol. 2004, 15, 411-418.
37. Sittinger, M.; Schultz, O.; Keyszer, G.; Minuth, W. W.; Burmester, G. R. Artificial tissues in perfusion culture. Int. J. Artif. Organs 1997, 20, 57-62.
38. Thissen, H.; Chang, K. Y.; Tebb, T. A.; Tsai, W. B.; Glattauer, V.; Ramshaw, J. A.; Werkmeister, J. A. Synthetic biodegradable microparticles for articular cartilage tissue engineering. J. Biomed. Mater. Res. A 2006, 77, 590-598.
39. Young, R. G.; Butler, D. L.; Weber, W.; Caplan, A. I.; Gordon, S. L.; Fink, D. J. Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair. J. Orthop. Res. 1998, 16, 406-413.