Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Samazan, Frédéric ^a,b   Rokbi, Bachra ^c   Seguin, Delphine ^c   Telles, Fabienne ^c   Gautier, Valérie ^d   Richarme, Gilbert ^d   Chevret, Didier ^a   Varela, Paloma Fernández ^e   Velours, Christophe ^e   Poquet, Isabelle ^a,f  

^a UMR1319 MICALIS   (France)

^b INSTITUT CURIE   (France)

^c SANOFI PASTEUR   (France)

^d INSTITUT JACQUES MONOD   (France)

^e CNRS   (France)

^f INSTITUT PASTEUR   (France)

Author keywords

Cell factory; Chaperone; HtrA family; Lactococcus lactis; Secretion; Soluble recombinant protein; Staphylococcus aureus antigen

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL PROTEIN; CHAPERONE; POLYCLONAL ANTIBODY; PROTEIN HTRA; PROTEIN USP45; RECOMBINANT PROTEIN; SERINE PROTEINASE; UNCLASSIFIED DRUG; BACTERIAL VACCINE; PEPTIDE HYDROLASE;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CIRCULAR DICHROISM; CONTROLLED STUDY; CULTURE MEDIUM; EXPRESSION VECTOR; GENE EXPRESSION; IMMUNIZATION; IMMUNOGENICITY; LACTOCOCCUS LACTIS; LIGHT SCATTERING; MASS SPECTROMETRY; MOLECULAR CLONING; MOUSE; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN SECRETION; SIZE EXCLUSION CHROMATOGRAPHY; STAPHYLOCOCCUS AUREUS; THERMOSTABILITY; WESTERN BLOTTING; WILD TYPE; ANIMAL; CHEMISTRY; ENZYMOLOGY; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; STAPHYLOCOCCAL INFECTIONS;

ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BACTERIAL VACCINES; HUMANS; LACTOCOCCUS LACTIS; MICE; PEPTIDE HYDROLASES; PROTEIN FOLDING; RECOMBINANT PROTEINS; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 84977640703     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0271-z     Document Type: Article

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