Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66

Microbial Cell Factories

Volumn 15, Issue 1, 2016, Pages

  Huang, Huiming ^a   Hou, Lukuan ^a   Li, Huayue ^a   Qiu, Yanhong ^a   Ju, Jianhua ^b   Li, Wenli ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

Author keywords

Cryptic gene cluster; Deepsea derived Streptomyces; Type III polyketide synthase (PKS); Violapyrones (VLPs); whiB like (wbl) gene

Indexed keywords

2 PYRONE DERIVATIVE; ANTIINFECTIVE AGENT; POLYKETIDE SYNTHASE; UNCLASSIFIED DRUG; VIOLAPYRONE A; VIOLAPYRONE B; VIOLAPYRONE C; VIOLAPYRONE H; BACTERIAL PROTEIN; POLYKETIDE; SEA WATER;

ACTINOBACTERIA; ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BACTERIAL STRUCTURES; CONTROLLED STUDY; DEEP SEA SPECIES; DRUG ISOLATION; DRUG STRUCTURE; GENE CLUSTER; GENE DELETION; GENE EXPRESSION; GENE INACTIVATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; REGULATOR GENE; STREPTOMYCES SOMALIENSIS; VIOA GENE; VIOB GENE; WBL GENE; WBLC GENE; WBLE GENE; WBLH GENE; WBLI GENE; WBLK GENE; WHID GENE; WHIG GENE; WHIH GENE; WHII GENE; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MULTIGENE FAMILY; PLASMID; STREPTOMYCES; SUPPLY AND DISTRIBUTION;

BACTERIAL PROTEINS; GENE DELETION; MULTIGENE FAMILY; PLASMIDS; POLYKETIDE SYNTHASES; POLYKETIDES; SEAWATER; STREPTOMYCES;

EID: 84976417863     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0515-6     Document Type: Article

References (35)

1. Fenical W, Jensen PR. Developing a new resource for drug discovery: marine actinomycete bacteria. Nat Chem Biol. 2006;2:666-73.
2. Jensen PR, Chavarria KL, Fenical W, Moore BS, Ziemert N. Challenges and triumphs to genomics-based natural product discovery. J Ind Microbiol Biot. 2014;41:203-9.
3. Bachmann BO, Van Lanen SG, Baltz RH. Microbial genome mining for accelerated natural products discovery: is a renaissance in the making? J Ind Microbiol Biot. 2014;41:175-84.
4. Hopwood D. Small things considered: the tip of the iceberg. 2008.
5. Liu G, Chater KF, Chandra G, Niu G, Tan H. Molecular regulation of antibiotic biosynthesis in Streptomyces. Microbiol Mol Biol Rev. 2013;77:112-43.
6. Rutledge PJ, Challis GL. Discovery of microbial natural products by activation of silent biosynthetic gene clusters. Nature Rev Microbiol. 2015;13:509-23.
7. Romero-Rodríguez A, Robledo-Casados I, Sánchez S. An overview on transcriptional regulators in Streptomyces. BBA-Gene Regulatory Mechanisms. 2015;1849:1017-39.
8. Fowler-Goldsworthy K, Gust B, Mouz S, Chandra G, Findlay KC, Chater KF. The actinobacteria-specific gene wblA controls major developmental transitions in Streptomyces coelicolor A3 (2). Microbiology. 2011;157:1312-28.
9. Zheng F, Long Q, Xie J. The function and regulatory network of WhiB and WhiB-like protein from comparative genomics and systems biology perspectives. Cell Biochem Biophys. 2012;63:103-8.
10. Soliveri J, Gomez J, Bishai W, Chater K. Multiple paralogous genes related to the Streptomyces coelicolor developmental regulatory gene whiB are present in Streptomyces and other actinomycetes. Microbiology. 2000;146:333-43.
11. Nah JH, Park SH, Yoon HM, Choi SS, Lee CH, Kim ES. Identification and characterization of wblA-dependent tmcT regulation during tautomycetin biosynthesis in Streptomyces sp. CK4412. Biotechnol Adv. 2012;30:202-9.
12. Noh JH, Kim SH, Lee HN, Lee SY, Kim ES. Isolation and genetic manipulation of the antibiotic down-regulatory gene, wblA ortholog for doxorubicin-producing Streptomyces strain improvement. Appl Microbiol and Biot. 2010;86:1145-53.
13. ch. Appl Environ Microbiol. 2014;80:6879-87.
14. Lu C, Liao G, Zhang J, Tan H. Identification of novel tylosin analogues generated by a wblA disruption mutant of Streptomyces ansochromogenes. Microb Cell Fact. 2015;14:1.
15. Zhang J, Jiang Y, Cao Y, Liu J, Zheng D, Chen X, Han L, Jiang C, Huang X. Violapyrones A-G, α-pyrone derivatives from Streptomyces violascens isolated from Hylobates hoolock feces. J Nat Prod. 2013;76:2126-30.
16. Tang X, Eitel K, Kaysser L, Kulik A, Grond S, Gust B. A two-step sulfation in antibiotic biosynthesis requires a type III polyketide synthase. Nat Chem Biol. 2013;9:610-5.
17. Nakano C, Ozawa H, Akanuma G, Funa N, Horinouchi S. Biosynthesis of aliphatic polyketides by type III polyketide synthase and methyltransferase in Bacillus subtilis. J Bacteriol. 2009;191:4916-23.
18. Song L, Barona-Gomez F, Corre C, Xiang L, Udwary DW, Austin MB, Noel JP, Moore BS, Challis GL. Type III polyketide synthase β-ketoacyl-ACP starter unit and ethylmalonyl-CoA extender unit selectivity discovered by Streptomyces coelicolor genome mining. J Am Chem Soc. 2006;128:14754-5.
19. Aizawa T, Kim SY, Takahashi S, Koshita M, Tani M, Futamura Y, Osada H, Funa N. Alkyldihydropyrones, new polyketides synthesized by a type III polyketide synthase from Streptomyces reveromyceticus. J Antibiot (Tokyo). 2014;67(12):819-23.
20. Funa N, Ozawa H, Hirata A, Horinouchi S. Phenolic lipid synthesis by type III polyketide synthases is essential for cyst formation in Azotobacter vinelandii. Proc Natl Acad Sci USA. 2006;103:6356-61.
21. Katsuyama Y, Ohnishi Y. Type III polyketide synthases in microorganisms. Methods Enzymol. 2012;515:359-77.
22. Morita H, Yamashita M, Shi SP, Wakimoto T, Kondo S, Kato R, Sugio S, Kohno T, Abe I. Synthesis of unnatural alkaloid scaffolds by exploiting plant polyketide synthase. Proc Natl Acad Sci USA. 2011;108:13504-9.
23. Chemler JA, Buchholz TJ, Geders TW, Akey DL, Rath CM, Chlipala GE, Smith JL, Sherman DH. Biochemical and structural characterization of germicidin synthase: analysis of a type III polyketide synthase that employs acyl-ACP as a starter unit donor. J Am Chem Soc. 2012;134:7359-66.
24. Kang SH, Huang J, Lee HN, Hur YA, Cohen SN, Kim ES. Interspecies DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces. J Bacteriol. 2007;189:4315-9.
25. gh influences morphogenesis and moenomycin production. Biotechnol Lett. 2011;33:2481-6.
26. Shin HJ, Lee HS, Lee JS, Shin J, Lee MA, Lee HS, Lee YJ, Yun J, Kang JS. Violapyrones H and I, new cytotoxic compounds isolated from Streptomyces sp. associated with the marine starfish Acanthaster planci. Mar Drugs. 2014;12:3283-91.
27. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1982.
28. E is required for normal cell wall structure in Streptomyces coelicolor A3 (2). J Bacteriol. 1999;181:204-11.
29. Gust B, Challis GL, Fowler K, Kieser T, Chater KF. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci USA. 2003;100:1541-6.
30. Zhang Y, Huang H, Xu S, Wang B, Ju J, Tan H, Li W. Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact. 2015;14:64.
31. Weber T, Blin K, Duddela S, Krug D, Kim H, Bruccoleri R, Lee S, Fischbach M, Muller R, Wohlleben W, Breitling R, Takano E, Medema M. antiSMASH 3.0-a comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res. 2015;43(1):W237-43.
32. Ishikawa J, Hotta K. FramePlot: a new implementation of the frame analysis for predicting protein-coding regions in bacterial DNA with a high G+C content. FEMS Microbiol Lett. 1999;174:251-3.
33. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403-10.
34. Gust B, Chandra G, Jakimowicz D, Yuqing T, Bruton CJ, Chater KF. Lambda red-mediated genetic manipulation of antibiotic-producing Streptomyces. Adv Appl Microbiol. 2004;54:107-28.
35. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA. Practical Streptomyces genetics. Norwich: John Innes Foundation; 2000.