A scalable strategy for high-throughput GFP tagging of endogenous human proteins

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 25, 2016, Pages E3501-E3508

  Leonetti, Manuel D ^a,b   Sekine, Sayaka ^b   Kamiyama, Daichi ^b   Weissman, Jonathan S ^a,b   Huang, Bo ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

CRISPR Cas9; Genome engineering; GFP library

Indexed keywords

CASPASE 9; RIBONUCLEOPROTEIN; GREEN FLUORESCENT PROTEIN; PROTEIN;

ARTICLE; CONTROLLED STUDY; ELECTROPORATION; FLOW CYTOMETRY; FLUORESCENCE; GENE TARGETING; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; FLUORESCENCE MICROSCOPY; GENETICS; HEK293 CELL LINE; HIGH THROUGHPUT SCREENING; PROCEDURES;

ELECTROPORATION; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROSCOPY, FLUORESCENCE; PROTEINS;

EID: 84975744753     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1606731113     Document Type: Article

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