The scanning electron microscope in microbiology and diagnosis of infectious disease

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Golding, Christine G ^a   Lamboo, Lindsey L ^a,b   Beniac, Daniel R ^a   Booth, Timothy F ^a,b  

^a PUBLIC HEALTH AGENCY OF CANADA   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

IONIC LIQUID;

ANIMAL; CELL LINE; COMMUNICABLE DISEASE; DEVICES; EBOLAVIRUS; HUMAN; PROCEDURES; SCANNING ELECTRON MICROSCOPY; SPECIMEN HANDLING; ULTRASTRUCTURE; VACCINIA VIRUS; VIROLOGY; VIRUS;

ANIMALS; CELL LINE; COMMUNICABLE DISEASES; EBOLAVIRUS; HUMANS; IONIC LIQUIDS; MICROSCOPY, ELECTRON, SCANNING; SPECIMEN HANDLING; VACCINIA VIRUS; VIRUSES;

EID: 84969988571     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep26516     Document Type: Article

References (34)

1. van Rooyen, C. E. & SCOTT, G. D. Smallpox diagnosis with special reference to electron microscopy. Can. J. Public Health 39, 467-477 (1948).
2. Goldsmith, C. S. & Miller, S. E. Modern uses of electron microscopy for detection of viruses. Clin. Microbiol. Rev. 22, 552-563, doi: 10.1128/CMR.00027-09 (2009).
3. Hazelton, P. R. & Gelderblom, H. R. Electron microscopy for rapid diagnosis of infectious agents in emergent situations. Emerg. Infect. Dis. 9, 294-303 (2003).
4. Nitsche, A., Gelderblom, H. R., Eisendle, K., Romani, N. & Pauli, G. Pitfalls in diagnosing human poxvirus infections. J. Clin. Virol. 38, 165-168 (2007).
5. Biel, S. S. et al. Detection of human polyomaviruses in urine from bone marrow transplant patients: comparison of electron microscopy with PCR. Clin. Chem. 50, 306-312 (2004).
6. Biel, S. S. & Gelderblom, H. R. Diagnostic electron microscopy is still a timely and rewarding method. J. Clin. Virol. 13, 105-119 (1999).
7. Beniac, D. R., Siemens, C. G., Wright, C. J. & Booth, T. F. A filtration based technique for simultaneous SEM and TEM sample preparation for the rapid detection of pathogens. Viruses. 6, 3458-3471, doi: 10.3390/v6093458 (2014).
8. Beniac, D. R., Hiebert, S. L., Siemens, C. G., Corbett, C. R. & Booth, T. F. A mobile biosafety microanalysis system for infectious agents. Sci. Rep. 5, 9505, doi: 10.1038/srep09505 (2015).
9. Thiberge, S. et al. Scanning electron microscopy of cells and tissues under fully hydrated conditions. Proc. Natl. Acad. Sci. USA 101, 3346-3351 (2004).
10. de Jonge, N., Peckys, D. B., Kremers, G. J. & Piston, D. W. Electron microscopy of whole cells in liquid with nanometer resolution. Proc. Natl. Acad. Sci. USA 106, 2159-2164, doi: 10.1073/pnas.0809567106 (2009).
11. Ogura, T. Nanoscale analysis of unstained biological specimens in water without radiation damage using high-resolution frequency transmission electric-field system based on FE-SEM. Biochem. Biophys. Res. Commun. 459, 521-528, doi: 10.1016/j.bbrc.2015.02.140 (2015).
12. Bergmans, L., Moisiadis, P., Van, M. B., Quirynen, M. & Lambrechts, P. Microscopic observation of bacteria: review highlighting the use of environmental SEM. Int. Endod. J. 38, 775-788 (2005).
13. Walther, P. & Muller, M. Biological ultrastructure as revealed by high resolution cryo-SEM of block faces after cryo-sectioning. J. Microsc. 196, 279-287 (1999).
14. Hayles, M. F., Stokes, D. J., Phifer, D. & Findlay, K. C. A technique for improved focused ion beam milling of cryo-prepared life science specimens. J. Microsc. 226, 263-269 (2007).
15. Katsen-Globa, A., Puetz, N., Gepp, M. M., Neubauer, J. C. & Zimmermann, H. Study of SEM preparation artefacts with correlative microscopy: Cell shrinkage of adherent cells by HMDS-drying. Scanning, doi: 10.1002/sca.21310 (2016).
16. Beniac, D. R. et al. The organisation of Ebola virus reveals a capacity for extensive, modular polyploidy. PLoS. One. 7, e29608, doi: 10.1371/journal.pone.0029608 (2012).
17. Cotelesage, J. J. et al. Hepatitis B Virus Genotype G forms core-like particles with unique structural properties. J. Viral Hepat. 18, 443-448, doi: 10.1111/j.1365-2893.2010.01330.x (2010).
18. Frank, J. et al. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J. Struct. Biol. 116, 190-199 (1996).
19. Kuwabata, S., Kongkanand, A., Oyamatsu, D. & Torimoto, T. Observation of ionic liquid by scanning electron microscope. Chemistry Letters 35, 600-601, doi: 10.1246/cl.2006.600 (2006).
20. Dwiranti, A. et al. Chromosome observation by scanning electron microscopy using ionic liquid. Microsc. Res. Tech. 75, 1113-1118, doi: 10.1002/jemt.22038 (2012).
21. Takahashi, C., Shirai, T. & Fuji, M. FE-SEM observation of swelled seaweed using hydrophilic ionic liquid; 1-butyl-3-methylimidazolium tetrafluoroborate. Microsc. Res. Tech. 76, 66-71, doi: 10.1002/jemt.22137 (2013).
22. Ishigaki, Y. et al. Ionic liquid enables simple and rapid sample preparation of human culturing cells for scanning electron microscope analysis. Microsc. Res. Tech. 74, 415-420, doi: 10.1002/jemt.20924 (2011).
23. Kawai, K., Kaneko, K., Kawakami, H., Narushima, T. & Yonezawa, T. Simple pretreatment of non-conductive small hydrous biosamples with choline-type ionic liquid and membrane filter for microsample mounting. Colloids Surf. B Biointerfaces. 102, 9-12, doi: 10.1016/j.colsurfb.2012.08.019 (2013).
24. Torimoto, T., Tsuda, T., Okazaki, K. & Kuwabata, S. New frontiers in materials science opened by ionic liquids. Adv. Mater. 22, 1196-1221, doi: 10.1002/adma.200902184 (2010).
25. Arimoto, S., Oyamatsu, D., Torimoto, T. & Kuwabata, S. Development of in situ electrochemical scanning electron microscopy with ionic liquids as electrolytes. Chemphyschem. 9, 763-767, doi: 10.1002/cphc.200700758 (2008).
26. Klungthong, C. et al. The impact of primer and probe-template mismatches on the sensitivity of pandemic influenza A/H1N1/2009 virus detection by real-time RT-PCR. J Clin. Virol. 48, 91-95, doi: 10.1016/j.jcv.2010.03.012 (2010).
27. Pyne, M. T., Brown, K. L. & Hillyard, D. R. Evaluation of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test and identification of rare polymorphisms potentially affecting assay performance. J Clin. Microbiol. 48, 2852-2858, doi: 10.1128/JCM.00776-10 (2010).
28. Mahony, J. B. & Richardson, S. Molecular diagnosis of severe acute respiratory syndrome: the state of the art. J Mol. Diagn. 7, 551-559 (2005).
29. Gelderblom, H. R. & Hazelton, P. R. Specimen collection for electron microscopy. Emerg. Infect. Dis. 6, 433-434 (2000).
30. Goldstein, J. I. et al. Scanning Electron Microscopy and X-ray Microanalysis (Springer, New York 2003).
31. Levett, P. N. Leptospirosis. Clin. Microbiol. Rev. 14, 296-326 (2001).
32. Fàbrega, A. & Vila, J. Salmonella enterica serovar Typhimurium skills to succeed in the host: virulence and regulation. Clin. Microbiol. Rev. 26, 308-341, doi: 10.1128/CMR.00066-12 (2013).
33. Halfmann, P. et al. Replication-deficient ebolavirus as a vaccine candidate. J. Virol. 83, 3810-3815, doi: 10.1128/JVI.00074-09 (2009).
34. Abramoff, M. D., Magelhaes, P. J. & Ram, S. J. Image Processing with ImageJ. Biophotonics International 11, 36-42 (2004).