The impact of primer and probe-template mismatches on the sensitivity of pandemic influenza A/H1N1/2009 virus detection by real-time RT-PCR

Journal of Clinical Virology

Volumn 48, Issue 2, 2010, Pages 91-95

  Klungthong, Chonticha ^a   Chinnawirotpisan, Piyawan ^a   Hussem, Kittinun ^a   Phonpakobsin, Thipwipha ^a   Manasatienkij, Wudtichai ^a   Ajariyakhajorn, Chuanpis ^a   Rungrojcharoenkit, Kamonthip ^a   Gibbons, Robert V ^a   Jarman, Richard G ^a  

^a ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES   (Thailand)

Author keywords

Mismatches; Novel influenza A (H1N1) 2009; Primer; Probe; Real time; RT PCR

Indexed keywords

INFLUENZA VIRUS HEMAGGLUTININ;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE MUTATION; GENE SEQUENCE; HUMAN; INFLUENZA VIRUS A H1N1; MOLECULAR PHYLOGENY; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; VIRUS DETECTION; VIRUS TRANSMISSION;

ASIA, SOUTHEASTERN; BASE PAIR MISMATCH; CLUSTER ANALYSIS; DNA PRIMERS; GENETIC VARIATION; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

EID: 77951622408     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2010.03.012     Document Type: Article

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