Chromosomal mapping of the αMHC-MerCreMer transgene in mice reveals a large genomic deletion

Transgenic Research

Volumn 25, Issue 5, 2016, Pages 639-648

  Harkins, Stephanie ^a   Whitton, J Lindsay ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

A1CF; Cardiomyocytes; Heart; MerCreMer; Tamoxifen

Indexed keywords

APOBEC1 PROTEIN, MOUSE; APOLIPOPROTEIN B MESSENGER RNA EDITING ENZYME CATALYTIC POLYPEPTIDE 1; CRE RECOMBINASE; INTEGRASE; MINICHROMOSOME MAINTENANCE PROTEIN; TAMOXIFEN;

ANIMAL; CARDIAC MUSCLE CELL; CHROMOSOMAL MAPPING; DNA SEQUENCE; DRUG EFFECTS; GENE DELETION; GENETICS; GENOME; GENOMICS; METABOLISM; MOUSE; PROMOTER REGION; TRANSGENIC MOUSE;

ANIMALS; APOBEC-1 DEAMINASE; CHROMOSOME MAPPING; GENOME; GENOMICS; INTEGRASES; MICE; MICE, TRANSGENIC; MINICHROMOSOME MAINTENANCE PROTEINS; MYOCYTES, CARDIAC; PROMOTER REGIONS, GENETIC; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION; TAMOXIFEN;

EID: 84966549192     PISSN: 09628819     EISSN: 15739368     Source Type: Journal    
DOI: 10.1007/s11248-016-9960-6     Document Type: Article

References (22)

1. Aldhous MC, Abu BS, Prescott NJ, Palla R, Soo K, Mansfield JC, Mathew CG, Satsangi J, Armour JA (2010) Measurement methods and accuracy in copy number variation: failure to replicate associations of beta-defensin copy number with Crohn’s disease. Hum Mol Genet 19:4930–4938
2. Althof N, Harkins S, Kemball CC, Flynn CT, Alirezaei M, Whitton JL (2014) In vivo ablation of type I interferon receptor from cardiomyocytes delays coxsackieviral clearance and accelerates myocardial disease. J Virol 88:5087–5099
3. Armour JA, Palla R, Zeeuwen PL, Den HM, Schalkwijk J, Hollox EJ (2007) Accurate, high-throughput typing of copy number variation using paralogue ratios from dispersed repeats. Nucleic Acids Res 35:e19
4. Bersell K, Choudhury S, Mollova M, Polizzotti BD, Ganapathy B, Walsh S, Wadugu B, Arab S, Kuhn B (2013) Moderate and high amounts of tamoxifen in αMHC-MerCreMer mice induce a DNA damage response, leading to heart failure and death. Dis Model Mech 6:1459–1469
5. Blanc V, Henderson JO, Newberry EP, Kennedy S, Luo J, Davidson NO (2005) Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality. Mol Cell Biol 25:7260–7269
6. Chiang C, Jacobsen JC, Ernst C, Hanscom C, Heilbut A, Blumenthal I, Mills RE, Kirby A, Lindgren AM, Rudiger SR, McLaughlan CJ, Bawden CS, Reid SJ, Faull RL, Snell RG, Hall IM, Shen Y, Ohsumi TK, Borowsky ML, Daly MJ, Lee C, Morton CC, MacDonald ME, Gusella JF, Talkowski ME (2012) Complex reorganization and predominant non-homologous repair following chromosomal breakage in karyotypically balanced germline rearrangements and transgenic integration. Nat Genet 44(390–7):S1
7. Dur S, Krause K, Pluntke N, Greeve J (2004) Gene structure and expression of the mouse APOBEC-1 complementation factor: multiple transcriptional initiation sites and a spliced variant with a premature stop translation codon. Biochim Biophys Acta 1680:11–23
8. Guescini M, Sisti D, Rocchi MB, Stocchi L, Stocchi V (2008) A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition. BMC Bioinform 9:326
9. Hall ME, Smith G, Hall JE, Stec DE (2011) Systolic dysfunction in cardiac-specific ligand-inducible MerCreMer transgenic mice. Am J Physiol Heart Circ Physiol 301:H253–H260
10. Hougen K, Aronsen JM, Stokke MK, Enger U, Nygard S, Andersson KB, Christensen G, Sejersted OM, Sjaastad I (2010) Cre-loxP DNA recombination is possible with only minimal unspecific transcriptional changes and without cardiomyopathy in Tg(αMHC-MerCreMer) mice. Am J Physiol Heart Circ Physiol 299:H1671–H1678
11. Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA (2009) Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12–15
12. Lexow J, Poggioli T, Sarathchandra P, Santini MP, Rosenthal N (2013) Cardiac fibrosis in mice expressing an inducible myocardial-specific Cre driver. Dis Model Mech 6:1470–1476
13. Lobanenkov VV, Nicolas RH, Adler VV, Paterson H, Klenova EM, Polotskaja AV, Goodwin GH (1990) A novel sequence-specific DNA binding protein which interacts with three regularly spaced direct repeats of the CCCTC-motif in the 5′-flanking sequence of the chicken c-myc gene. Oncogene 5:1743–1753
14. Mehta A, Kinter MT, Sherman NE, Driscoll DM (2000) Molecular cloning of apobec-1 complementation factor, a novel RNA-binding protein involved in the editing of apolipoprotein B mRNA. Mol Cell Biol 20:1846–1854
15. Molkentin JD, Robbins J (2009) With great power comes great responsibility: using mouse genetics to study cardiac hypertrophy and failure. J Mol Cell Cardiol 46:130–136
16. (Myh6-cre/Esr1*)1Jmk allele. http://www.informatics.jax.org/reference/allele/MGI:3050453
17. Ong CT, Corces VG (2014) CTCF: an architectural protein bridging genome topology and function. Nat Rev Genet 15:234–246
18. Rochat B (2005) Role of cytochrome P450 activity in the fate of anticancer agents and in drug resistance: focus on tamoxifen, paclitaxel and imatinib metabolism. Clin Pharmacokinet 44:349–366
19. Sohal DS, Nghiem M, Crackower MA, Witt SA, Kimball TR, Tymitz KM, Penninger JM, Molkentin JD (2001) Temporally regulated and tissue-specific gene manipulations in the adult and embryonic heart using a tamoxifen-inducible Cre protein. Circ Res 89:20–25
20. Srivastava A, Philip VM, Greenstein I, Rowe LB, Barter M, Lutz C, Reinholdt LG (2014) Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries. BMC Genom 15:367
21. Stephens PJ, Greenman CD, Fu B, Yang F, Bignell GR, Mudie LJ, Pleasance ED, Lau KW, Beare D, Stebbings LA, McLaren S, Lin ML, McBride DJ, Varela I, Nik-Zainal S, Leroy C, Jia M, Menzies A, Butler AP, Teague JW, Quail MA, Burton J, Swerdlow H, Carter NP, Morsberger LA, Iacobuzio-Donahue C, Follows GA, Green AR, Flanagan AM, Stratton MR, Futreal PA, Campbell PJ (2011) Massive genomic rearrangement acquired in a single catastrophic event during cancer development. Cell 144:27–40
22. VanBrocklin MW, Robinson JP, Whitwam T, Guilbeault AR, Koeman J, Swiatek PJ, Vande Woude GF, Khoury JD, Holmen SL (2009) Met amplification and tumor progression in Cdkn2a-deficient melanocytes. Pigment Cell Melanoma Res 22:454–460