Overexpression of ppc and lysC to improve the production of 4-hydroxyisoleucine and its precursor l-isoleucine in recombinant Corynebacterium glutamicum ssp. lactofermentum

Enzyme and Microbial Technology

Volumn 87-88, Issue , 2016, Pages 79-85

  Shi, Feng ^a   Fang, Huimin ^a   Niu, Tengfei ^a   Lu, Zhengke ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

4 Hydroxyisoleucine; Corynebacterium glutamicum ssp. lactofermentum; L isoleucine dioxygenase; LysC; Ppc

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CARBON; CLONING;

4-HYDROXYISOLEUCINE; BIOSYNTHESIS PATHWAYS; CONVERSION RATIO; CORYNEBACTERIUM GLUTAMICUM SSP. LACTOFERMENTUM; DIOXYGENASE GENES; DIOXYGENASES; LYSC; SENSITIZING ACTIVITY;

AMINO ACIDS;

4 HYDROXYISOLEUCINE; ASPARTATE KINASE; ASPARTIC ACID; CARBON; DIOXYGENASE; ISOLEUCINE; LEVO ISOLEUCINE DIOXYGENASE; PHOSPHOENOLPYRUVATE CARBOXYLASE; UNCLASSIFIED DRUG; 4-HYDROXYISOLEUCINE; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL STRAIN; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; FERMENTATION; GENE OVEREXPRESSION; NONHUMAN; PH; PRECURSOR; ANALOGS AND DERIVATIVES; BACTERIAL GENE; BIOSYNTHESIS; CARBON CYCLE; GENETICS; METABOLISM; UPREGULATION;

ASPARTATE KINASE; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; CARBON CYCLE; CORYNEBACTERIUM GLUTAMICUM; DIOXYGENASES; FERMENTATION; GENES, BACTERIAL; ISOLEUCINE; PHOSPHOENOLPYRUVATE CARBOXYLASE; RECOMBINANT PROTEINS; UP-REGULATION;

EID: 84964445507     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2016.04.008     Document Type: Article

References (31)

1. Fowden L., Pratt H.M., Smith A. 4-Hydroxyisoleucine from seed of Trigonella foenum-graecum. Photochemistry 1973, 12:1707-1711.
2. Sauvaire Y., Petit P., Broca C., Manteghetti M., Baissac Y., Fernandez-Alvarez J., Gross R., Roye M., Leconte A., Gomis R., Ribes G. 4-Hydroxyisoleucine: a novel amino acid potentiator of insulin secretion. Diabetes 1998, 47:206-210.
3. Broca C., Gross R., Petit P., Sauvaire Y., Manteghetti M., Tournier M., Masiello P., Gomis R., Ribes G. 4-Hydroxyisoleucine: experimental evidence of its insulinotropic and antidiabetic properties. Am. J. Physiol. 1999, 277:E617-E623.
4. Broca C., Breil V., Cruciani-Guglielmacci C., Manteghetti M., Rouault C., Derouet M., Rizkalla S., Pau B., Petit P., Ribes G., Ktorza A., Gross R., Reach G., Taouis M. Insulinotropic agent ID-1101 (4-hydroxyisoleucine) activates insulin signaling in rat. Am. J. Physiol. Endocrinol. Metab. 2004, 287:E463-E471.
5. Maurya C.K., Singh R., Jaiswal N., Venkateswarlu K., Narender T., Tamrakar A.K. 4-Hydroxyisoleucine ameliorates fatty acid-induced insulin resistance and inflammatory response in skeletal muscle cells. Mol. Cell. Endocrinol. 2014, 395:51-60.
6. Gao F., Du W., Zafar M.I., Shafqat R.A., Jian L., Cai Q., Lu F. 4-Hydroxyisoleucine ameliorates an insulin resistant-like state in 3T3-L1 adipocytes by regulating TACE/TIMP3 expression. Drug Des. Devel. Ther. 2015, 9:5727-5736.
7. Narender T., Puri A., Shweta, Khaliq T., Saxena R., Bhatia G., Chandra R. 4-hydroxyisoleucine an unusual amino acid as antidyslipidemic and antihyperglycemic agent. Bioorg. Med. Chem. Lett. 2006, 16:293-296.
8. Singh A.B., Tamarkar A.K., Narender T., Srivastava A.K. Antihyperglycaemic effect of an unusual amino acid (4-hydroxyisoleucine) in C57BL/KsJ-db/db mice. Nat. Prod. Res. 2010, 24:258-265.
9. Haeri M.R., Limaki H.K., White C.J., White K.N. Non-insulin dependent anti-diabetic activity of (2S 3R, 4S) 4-hydroxyisoleucine of fenugreek (Trigonella foenum graecum) in streptozotocin- induced type I diabetic rats. Phytomedicine 2012, 19:571-574.
10. Kodera T., Smirnov S.V., Samsonova N.N., Kozlov Y.I., Koyama R., Hibi M., Ogawa J., Yokozeki K., Shimizu S. A novel l-isoleucine hydroxylating enzyme l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine. Biochem. Biophys. Res. Commun. 2009, 390:506-510.
11. Hibi M., Kawashima T., Kodera T., Smirnov S.V., Sokolov P.M., Sugiyama M., Shimizu S., Yokozeki K., Ogawa J. Characterization of Bacillus thuringiensis l-isoleucine dioxygenase for production of useful amino acids. Appl. Environ. Microbiol. 2011, 77:6926-6930.
12. Ogawa J., Kodera T., Smirnov S.V., Hibi M., Samsonova N.N., Koyama R., Yamanaka H., Mano J., Kawashima T., Yokozeki K., Shimizu S. A novel l-isoleucine metabolism in Bacillus thuringiensis generating (2S,3R,4S)-4-hydroxyisoleucine, a potential insulinotropic and anti-obesity amino acid. Appl. Microbiol. Biotechnol. 2011, 89:1929-1938.
13. Smirnov S.V., Kodera T., Samsonova N.N., Kotlyarova V.A., Rushkevich N.Y., Kivero A.D., Sokolov P.M., Hibi M., Ogawa J., Shimizu S. Metabolic engineering of Escherichia coli to produce (2S 3R, 4S)-4-hydroxyisoleucine. Appl. Microbiol. Biotechnol. 2010, 88:719-726.
14. Kivero A.D., Novikova A.E., Smirnov S.V. Modification of E. coli central metabolism to optimize the biotransformation of l-isoleucine into 4-hydroxyisoleucine by enzymatic hydroxylation. Appl. Biochem. Microbiol. 2012, 48:639-644.
15. Park J.H., Lee S.Y. Metabolic pathways and fermentative production of L-aspartate family amino acids. Biotechnol. J. 2010, 5:560-577.
16. Shi F., Niu T., Fang H. 4-Hydroxyisoleucine production of recombinant Corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation. Appl. Microbiol. Biotechnol. 2015, 99:3851-3863.
17. Shi F., Huan X., Wang X., Ning J. Overexpression of NAD kinases improves the l-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum. Enzyme Microb. Technol. 2012, 51:73-80.
18. Chen Z., Bommareddy R.R., Frank D., Rappert S., Zeng A.P. Deregulation of feedback inhibition of phosphoenolpyruvate carboxylase for improved lysine production in Corynebacterium glutamicum. Appl. Environ. Microbiol. 2014, 80:1388-1393.
19. Wanda M., Sawada K., Ogura K., Shimono Y., Hagiwara T., Sugimoto M., Onuki A., Yokota A. Effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in Corynebacterium glutamicum ATCC 13032. J. Biosci. Bioeng. 2016, 121:172-177.
20. Ikeda M., Ohnishi J., Hayashi M., Mitsuhashi S. A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient L-lysine production. J. Ind. Microbiol. Biotechnol. 2006, 33:610-615.
21. Hu J., Li Y., Zhang H., Tan Y., Wang X. Construction of a novel expression system for use in Corynebacterium glutamicum. Plasmid 2014, 75:18-26.
22. Shi F., Li K., Huan X., Wang X. Expression of NAD(H) kinase and glucose-6-phosphate dehydrogenase improve NADPH supply and l-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum. Appl. Biochem. Biotechnol. 2013, 171:504-521.
23. Kennerknecht N., Sahm H., Yen M.R., Patek M., Saier M.H., Eggeling L. Export of l-isoleucine from Corynebacterium glutamicum: a two-gene-encoded member of a new translocator family. J. Bacteriol. 2002, 184:3947-3956.
24. Shi F., Jiang J.J., Li Y.F., Li Y.X., Xie Y.L. Enhancement of γ-aminobutyric acid production in recombinant Corynebacterium glutamicum by co-expressing two glutamate decarboxylase genes from Lactobacillus brevis. J. Ind. Microbiol. Biotechnol. 2013, 40:1285-1296.
25. Eikmanns B.J., Follettie M.T., Griot M.U., Sinskey A.J. The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression. Mol. Gen. Genet. 1989, 218:330-339.
26. Peters-Wendisch P.G., Kreutzer C., Kalinowski J., Pátek M., Sahm H., Eikmanns B.J. Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene. Microbiology 1998, 144:915-927.
27. Peters-Wendisch P.G., Wendisch V.F., Paul S., Eikmanns B.J., Sahm H. Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum. Microbiology 1997, 143:1095-1103.
28. Sato H., Orishimo K., Shirai T., Hirasawa T., Nagahisa K., Shimizu H., Wachi M. Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum. J. Biosci. Bioeng. 2008, 106:51-58.
29. O'Regan M., Thierbach G., Bachmann B., Villeval D., Lepage P., Viret J.F., Lemoine Y. Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032. Gene 1989, 77:237-251.
30. Petersen S., de Graaf A.A., Eggeling L., Mollney M., Wiechert W., Sahm H. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J. Biol. Chem. 2000, 275:35932-35941.
31. Fu M., Nie Y., Mu X., Xu Y., Xiao R. A novel isoleucine dioxygenase and its expression in recombinant Escherichia coli for synthesis of 4-hydroxyisoleucine. Chem. Ind. Eng. Prog. 2014, 33:3037-3044.