Corrigendum: Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming (Nature Communications (2016) 7 (11208) DOI: 10.1038/ncomms11208);Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming

Nature Communications

Volumn 7, Issue , 2016, Pages

  Moreau, Thomas ^a,b,c   Evans, Amanda L ^a,c   Vasquez, Louella ^d   Tijssen, Marloes R ^a   Yan, Ying ^d   Trotter, Matthew W ^b,f   Howard, Daniel ^a,c   Colzani, Maria ^a,c   Arumugam, Meera ^a,c   Wu, Wing Han ^a,c   Dalby, Amanda ^a,c   Lampela, Riina ^c   Bouet, Guenaelle ^a,c   Hobbs, Catherine M ^a,c   Pask, Dean C ^a,c   Payne, Holly ^e   Ponomaryov, Tatyana ^e   Brill, Alexander ^e   Soranzo, Nicole ^d   Ouwehand, Willem H ^a   Pedersen, Roger A ^b,c   Ghevaert, Cedric ^a,c   more..

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c University of Cambridge   (United Kingdom)

^d WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^e UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^f Celgene Institute Translational Research Europe (CITRE)   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR FLI 1; TRANSCRIPTION FACTOR GATA 1; TRANSCRIPTION FACTOR TAL1; BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR; FLI1 PROTEIN, HUMAN; GATA1 PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; ONCOPROTEIN; TAL1 PROTEIN, HUMAN;

BLOOD; CELLS AND CELL COMPONENTS; CRYOPRESERVATION; GENETIC ENGINEERING; PROTEIN;

ARTICLE; BASIC RESEARCH; BLOOD CLOTTING; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL REPROGRAMMING TECHNIQUE; CELL STRUCTURE; CRYOPRESERVATION; GENE EXPRESSION; GENE REGULATORY NETWORK; GOOD MANUFACTURING PRACTICE; HUMAN; HUMAN CELL; IN VITRO STUDY; LARGE SCALE PRODUCTION; MEGAKARYOCYTE; PLURIPOTENT STEM CELL; PROTEIN PROTEIN INTERACTION; STEM CELL CULTURE; THROMBOCYTE FUNCTION; THROMBOCYTOPOIESIS; UMBILICAL CORD BLOOD; CELL CULTURE TECHNIQUE; CYTOLOGY; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; LENTIVIRINAE; METABOLISM; MICROARRAY ANALYSIS; NUCLEAR REPROGRAMMING; PROCEDURES; REPORTER GENE; SIGNAL TRANSDUCTION; THROMBOCYTE; TRANSGENE;

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; BLOOD PLATELETS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLULAR REPROGRAMMING; CRYOPRESERVATION; GATA1 TRANSCRIPTION FACTOR; GENE EXPRESSION REGULATION; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; LENTIVIRUS; MEGAKARYOCYTES; MICROARRAY ANALYSIS; PLURIPOTENT STEM CELLS; PROTO-ONCOGENE PROTEIN C-FLI-1; PROTO-ONCOGENE PROTEINS; SIGNAL TRANSDUCTION; TRANSDUCTION, GENETIC; TRANSGENES;

EID: 84964026871     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms15076     Document Type: Erratum

References (68)

1. Kaushansky, K. Historical review: megakaryopoiesis and thrombopoiesis. Blood 111, 981-986 (2008).
2. Michelson, A. D. Platelets 3rd Edn. (Academic Press, 2013).
3. Machlus, K. R., & Italiano, Jr J. E. The incredible journey: from megakaryocyte development to platelet formation. J. Cell Biol. 201, 785-796 (2013).
4. Junt, T., et al. Dynamic visualization of thrombopoiesis within bone marrow. Science 317, 1767-1770 (2007).
5. Estcourt, L. J., et al. Platelet transfusions in haematology patients: are we using them appropriately? Vox Sang. 103, 284-293 (2012).
6. Williamson, L. M., & Devine, D. V. Challenges in the management of the blood supply. Lancet 381, 1866-1875 (2013).
7. Stroncek, D. F., & Rebulla, P. Platelet transfusions. Lancet 370, 427-438 (2007).
8. Brown, C. J., & Navarrete, C. V. Clinical relevance of the HLA system in blood transfusion. Vox Sang. 101, 93-105 (2011).
9. Thomson, J. A., et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998).
10. Takahashi, K., et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007).
11. Murry, C. E., & Keller, G. Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132, 661-680 (2008).
12. Wu, S. M., & Hochedlinger, K. Harnessing the potential of induced pluripotent stem cells for regenerative medicine. Nat. Cell Biol. 13, 497-505 (2011).
13. Takayama, N., & Eto, K. Pluripotent stem cells reveal the developmental biology of human megakaryocytes and provide a source of platelets for clinical application. Cell. Mol. Life. Sci. 69, 3419-3428 (2012).
14. Corash, L., & Lin, L. Novel processes for inactivation of leukocytes to prevent transfusion-Associated graft-versus-host disease. Bone Marrow Transplant. 33, 1-7 (2004).
15. Gaur, M., et al. Megakaryocytes derived from human embryonic stem cells: a genetically tractable system to study megakaryocytopoiesis and integrin function. J. Thromb. Haemost. 4, 436-442 (2006).
16. Klimchenko, O., et al. A common bipotent progenitor generates the erythroid and megakaryocyte lineages in embryonic stem cell-Derived primitive hematopoiesis. Blood 114, 1506-1517 (2009).
17. Takayama, N., et al. Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells. J. Exp. Med. 207, 2817-2830 (2010).
18. Pick, M., Azzola, L., Osborne, E., Stanley, E. G., & Elefanty, A. G. Generation of megakaryocytic progenitors from human embryonic stem cells in a feeder-and serum-free medium. PLoS ONE 8, e55530 (2013).
19. Feng, Q., et al. Scalable generation of universal platelets from human induced pluripotent stem cells. Stem Cell Rep. 3, 817-831 (2014).
20. Nakamura, S., et al. Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell 14, 535-548 (2014).
21. Cherry, A. B., & Daley, G. Q. Reprogramming cellular identity for regenerative medicine. Cell 148, 1110-1122 (2012).
22. Zaret, K. S., & Carroll, J. S. Pioneer transcription factors: establishing competence for gene expression. Genes Dev. 25, 2227-2241 (2011).
23. Watkins, N. A., et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells. Blood 113, e1-E9 (2009).
24. Hu, Z., et al. VisANT 4.0: integrative network platform to connect genes, drugs, diseases and therapies. Nucleic Acids Res. 41, W225-W231 (2013).
25. Bernardo, A. S., et al. BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages. Cell Stem Cell 9, 144-155 (2011).
26. Tijssen, M. R., et al. Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators. Dev. Cell 20, 597-609 (2011).
27. Takayama, N., et al. Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors. Blood 111, 5298-5306 (2008).
28. Liu, Z. J., & Sola-Visner, M. Neonatal and adult megakaryopoiesis. Curr. Opin. Hematol. 18, 330-337 (2011).
29. Mootha, V. K., et al. PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat. Genet. 34, 267-273 (2003).
30. Bluteau, O., et al. Developmental changes in human megakaryopoiesis. J. Thromb. Haemost. 11, 1730-1741 (2013).
31. Thon, J. N., et al. Cytoskeletal mechanics of proplatelet maturation and platelet release. J. Cell Biol. 191, 861-874 (2010).
32. Gardiner, E. E., & Andrews, R. K. Platelet receptor expression and shedding: glycoprotein Ib-IX-V and glycoprotein VI. Transfus. Med. Rev. 28, 56-60 (2014).
33. Hu, Z., & Yang, Y. G. Full reconstitution of human platelets in humanized mice after macrophage depletion. Blood 120, 1713-1716 (2012).
34. Hartley, P. S., Savill, J., & Brown, S. B. The death of human platelets during incubation in citrated plasma involves shedding of CD42b and aggregation of dead platelets. Thromb. Haemost. 95, 100-106 (2006).
35. Versteeg, H. H., Heemskerk, J. W., Levi, M., & Reitsma, P. H. New fundamentals in hemostasis. Physiol. Rev. 93, 327-358 (2013).
36. Tynngard, N., Wallstedt, M., Sodergren, A. L., Faxalv, L., & Ramstrom, S. Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads. Platelets 26, 177-185 (2015).
37. Strauss, T., Sidlik-Muskatel, R., & Kenet, G. Developmental hemostasis: primary hemostasis and evaluation of platelet function in neonates. Semin. Fetal Neonatal Med. 16, 301-304 (2011).
38. De Cuyper, I. M., et al. A novel flow cytometry-based platelet aggregation assay. Blood 121, e70-E80 (2013).
39. Darbousset, R., et al. P2X1 expressed on polymorphonuclear neutrophils and platelets is required for thrombosis in mice. Blood 124, 2575-2585 (2014).
40. Dore, L. C., & Crispino, J. D. Transcription factor networks in erythroid cell and megakaryocyte development. Blood 118, 231-239 (2011).
41. Pimanda, J. E., et al. Gata2, Fli1, and Scl form a recursively wired generegulatory circuit during early hematopoietic development. Proc. Natl. Acad. Sci. USA 104, 17692-17697 (2007).
42. Fujiwara, Y., Chang, A. N., Williams, A. M., & Orkin, S. H. Functional overlap of GATA-1 and GATA-2 in primitive hematopoietic development. Blood 103, 583-585 (2004).
43. Tijssen, M. R., & Ghevaert, C. Transcription factors in late megakaryopoiesis and related platelet disorders. J. Thromb. Haemost. 11, 593-604 (2013).
44. Heffner, G. C., Clutter, M. R., Nolan, G. P., & Weissman, I. L. Novel hematopoietic progenitor populations revealed by direct assessment of GATA1 protein expression and cMPL signaling events. Stem Cells 29, 1774-1782 (2011).
45. Iwasaki, H., et al. GATA-1 converts lymphoid and myelomonocytic progenitors into the megakaryocyte/erythrocyte lineages. Immunity 19, 451-462 (2003).
46. Valtieri, M., et al. Enforced TAL-1 expression stimulates primitive, erythroid and megakaryocytic progenitors but blocks the granulopoietic differentiation program. Cancer Res. 58, 562-569 (1998).
47. Moussa, O., et al. Thrombocytopenia in mice lacking the carboxy-Terminal regulatory domain of the Ets transcription factor Fli1. Mol. Cell. Biol. 30, 5194-5206 (2010).
48. Liu, F., Walmsley, M., Rodaway, A., & Patient, R. Fli1 acts at the top of the transcriptional network driving blood and endothelial development. Curr. Biol. 18, 1234-1240 (2008).
49. Ismailoglu, I., Yeamans, G., Daley, G. Q., Perlingeiro, R. C., & Kyba, M. Mesodermal patterning activity of SCL. Exp. Hematol. 36, 1593-1603 (2008).
50. Real, P. J., et al. SCL/TAL1 regulates hematopoietic specification from human embryonic stem cells. Mol. Ther. 20, 1443-1453 (2012).
51. Toscano, M. G., et al. SCL/TAL1-mediated transcriptional network enhances megakaryocytic specification of human embryonic stem cells. Mol. Ther. 23, 158-170 (2015).
52. Elcheva, I., et al. Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators. Nat. Commun. 5, 4372 (2014).
53. Gieger, C., et al. New gene functions in megakaryopoiesis and platelet formation. Nature 480, 201-208 (2011).
54. Yamamoto, R., et al. Clonal analysis unveils self-renewing lineage-restricted progenitors generated directly from hematopoietic stem cells. Cell 154, 1112-1126 (2013).
55. Noh, J. Y., et al. Inducible Gata1 suppression expands megakaryocyte-Erythroid progenitors from embryonic stem cells. J. Clin. Invest. 125, 2369-2374 (2015).
56. Wang, Y., et al. Comparative analysis of human ex vivo-generated platelets vs. megakaryocyte-generated platelets in mice: a cautionary tale. Blood 125, 3627-3636 (2015).
57. Lu, S. J., et al. Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice. Cell Res. 21, 530-545 (2011).
58. Di Buduo, C. A., et al. Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies. Blood 125, 2254-2264 (2015).
59. Thon, J. N., et al. Platelet bioreactor-on-A-chip. Blood 124, 1857-1867 (2014).
60. Nakagawa, Y., et al. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-Derived megakaryocytes. Exp. Hematol. 41, 742-748 (2013).
61. Albers, C. A., et al. Exome sequencing identifies NBEAL2 as the causative gene for gray platelet syndrome. Nat. Genet. 43, 735-737 (2011).
62. Albers, C. A., et al. Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome. Nat. Genet. 44, 435-439 (2012).
63. Vallier, L., & Pedersen, R. Differentiation of human embryonic stem cells in adherent and in chemically defined culture conditions. Curr. Protoc. Stem Cell Biol. Chapter 1, Unit 1D 4 1-1D 4 7 (2008).
64. Skepper, J. N. Immunocytochemical strategies for electron microscopy: choice or compromise. J. Microsc. 199, 1-36 (2000).
65. Cazenave, J. P., et al. Preparation of washed platelet suspensions from human and rodent blood. Methods Mol. Biol. 272, 13-28 (2004).
66. Newman, P. J., Aster, R., & Boylan, B. Human platelets circulating in mice: applications for interrogating platelet function and survival, the efficacy of antiplatelet therapeutics, and the molecular basis of platelet immunological disorders. J. Thromb. Haemost. 5, 305-309 (2007).
67. Ghevaert, C., et al. Recombinant HPA-1a antibody therapy for treatment of fetomaternal alloimmune thrombocytopenia: proof of principle in human volunteers. Blood 122, 313-320 (2013).
68. de Witt, S. M., et al. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat. Commun. 5, 4257 (2014).