A facile and efficient transposon mutagenesis method for generation of multi-codon deletions in protein sequences

Journal of Biotechnology

Volumn 227, Issue , 2016, Pages 27-34

  Liu, Shu su ^a   Wei, Xuan ^a   Ji, Qun ^a   Xin, Xiu ^a   Jiang, Biao ^a   Liu, Jia ^a,b  

^a SHANGHAITECH UNIVERSITY   (China)

^b University of Maryland   (United States)

Author keywords

Asymmetric transposon; Codon deletions; Green fluorescent protein; Mutant library; Reading frame selection

Indexed keywords

BIOCHEMICAL ENGINEERING; CONVOLUTIONAL CODES; FLUORESCENCE; GENETIC ENGINEERING; MUTAGENESIS;

ASYMMETRIC TRANSPOSON; CODON DELETIONS; FRAME SELECTION; GREEN FLUORESCENT PROTEIN; MUTANT LIBRARIES;

PROTEINS;

GREEN FLUORESCENT PROTEIN; PROTEIN; TRANSPOSASE; CODON; TRANSPOSON;

AMINO ACID SEQUENCE; ARTICLE; CODON; CONTROLLED STUDY; DELETION MUTANT; DNA FLANKING REGION; FLUORESCENCE; GENE DELETION; GENETIC SCREENING; INVERSE POLYMERASE CHAIN REACTION; MOLECULAR LIBRARY; MUTAGENESIS; MUTATIONAL ANALYSIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ENGINEERING; TERMINAL SEQUENCE; TRANSPOSON; GENE EXPRESSION REGULATION; GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROCEDURES; RESTRICTION MAPPING;

AMINO ACID SEQUENCE; BASE SEQUENCE; CODON; DNA TRANSPOSABLE ELEMENTS; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SEQUENCE DELETION;

EID: 84962788705     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.03.038     Document Type: Article

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