A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: Mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach

Nucleic Acids Research

Volumn 33, Issue 12, 2005, Pages 1-8

  Poussu, Eini ^a   Jäntti, Jussi ^b   Savilahti, Harri ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; FUNGAL DNA; MSO1 PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; SEC1 PROTEIN; UNCLASSIFIED DRUG; MEMBRANE PROTEIN; MSO1 PROTEIN, S CEREVISIAE; MUNC18 PROTEIN; NERVE PROTEIN; VESICULAR TRANSPORT PROTEIN;

5' UNTRANSLATED REGION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIOPHAGE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA MODIFICATION; DNA TRANSPOSITION; EXPRESSION VECTOR; GENE CONSTRUCT; GENE DELETION; GENE FUNCTION; GENE MAPPING; GENETIC ENGINEERING; GENETIC MANIPULATION; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; RESTRICTION SITE; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; TRANSLATION REGULATION; TRANSPOSON; BACTERIOPHAGE MU; BINDING SITE; CHEMISTRY; EVALUATION; GENETICS; METABOLISM; METHODOLOGY; PLASMID; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE;

ENTEROBACTERIA PHAGE MU;

BACTERIOPHAGE MU; BINDING SITES; DNA TRANSPOSABLE ELEMENTS; MEMBRANE PROTEINS; MUNC18 PROTEINS; NERVE TISSUE PROTEINS; PLASMIDS; PROTEIN ENGINEERING; PROTEIN INTERACTION MAPPING; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE DELETION; VESICULAR TRANSPORT PROTEINS;

EID: 26444591575     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni102     Document Type: Article

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