N-terminal domain-deleted mu transposase exhibits increased transposition activity with low target site preference in modified buffers

Journal of Molecular Microbiology and Biotechnology

Volumn 17, Issue 1, 2009, Pages 30-40

  Kim, Yun Cheol ^a,b   Morrison, Sherie L ^a,b  

^a Department of MicrobiologyImmunology and Molecular Genetics ^*  (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Buffer composition; Mu Transposase; Target site preference; Transposition activity; Transposon

Indexed keywords

BUFFER; TRANSPOSASE; MU TRANSPOSASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIOPHAGE MU; BACTERIOPHAGE MUA; CARBOXY TERMINAL SEQUENCE; COMPARATIVE STUDY; DNA PURIFICATION; ENZYME ACTIVITY; GENE DELETION; GENE EXPRESSION; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; TRANSPOSON; WILD TYPE; GENE EXPRESSION REGULATION; GENETIC RECOMBINATION; GENETICS; METABOLISM; METHODOLOGY;

DNA TRANSPOSABLE ELEMENTS; MUTAGENESIS, INSERTIONAL; RECOMBINATION, GENETIC; SEQUENCE DELETION; TRANSPOSASES;

EID: 67649407712     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000178019     Document Type: Article

References (41)

1. Anton BP, Raleigh EA: Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease. J Bacteriol 2004;186:5699-5707.
2. Bajaj K, Chakrabarti P, Varadarajan R: Mutagenesis-based definitions and probes of residue burial in proteins. Proc Natl Acad Sci USA 2005;102:16221-16226.
3. Baker T, Mizuuchi K: DNA-promoted assembly of the active tetramer of the Mu transposase. Genes Dev 1992;6:2221-2232.
4. Baker T, Mizuuchi M, Mizuuchi K: Mu B protein allosterically activates strand transfer by the transposase of phage Mu. Cell 1991;65:1003-1013.
5. Baker TA, Kremenstova E, Luo L: Complete transposition requires four active monomers in the mu transposase tetramer. Genes Dev 1994;8:2416-2428.
6. Bishop J: A DNA sequence cleaved by restriction endonuclease R. EcoRI in only one strand. J Mol Biol 1979;128:545-549.
7. Castilho BA, Olfson P, Casadaban MJ: Plasmid insertion mutagenesis and lac gene fusion with minimu bacteriophage transposons. J Bacteriol 1984;158:488-495.
8. Chaconas G, Harshey RM: Transposition of Phage Mu DNA. Mobile DNA II. Washington, ASM Press, 2002, pp 384-402.
9. Chen Y, Yan J, Yang M, Wang J, Shen D: Expression of green fluorescent protein in Bacillus brevis under the control of a novel constitutive promoter F1 and insertion mutagenesis of F1 in Escherichia coli DH5alpha. FEMS Microbiol Lett 2003;229:111-117.
10. Clarke AR, Wigley DB, Chia WN, Barstow D, Atkinson T, Holbrook JJ: Site-directed mutagenesis reveals role of mobile arginine residue in lactate dehydrogenase catalysis. Nature 1986;324:699-702.
11. Clubb RT, Mizuuchi M, Huth JR, Omichinski JG, Savilahti H, Mizuuchi K, Clore GM, Gronenborn AM: The wing of the enhancer-binding domain of Mu phage transposase is flexible and is essential for efficient transposition. Proc Natl Acad Sci USA 1996a;93:1146-1150.
12. Conlan L, José T, Thornton K, Dupureur C: Modulating restriction endonuclease activities and specificities using neutral detergents. Biotechniques 1999;27:955-960.
13. Craigie R, Mizuuchi K: Transposition of Mu DNA: joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu. Cell 1987;51:493-501.
14. Craigie R, Mizuuchi M, Mizuuchi K: Site-specific recognition of the bacteriophage Mu ends by the Mu A protein. Cell 1984;39:387-394.
15. Fransen M, Vastiau I, Brees C, Brys V, Mannaerts GP, Van Veldhoven PP: Analysis of human Pex19p's domain structure by pentapeptide scanning mutagenesis. J Mol Biol 2005;346:1275-1286.
16. Haapa S, Suomalainen S, Eerikainen S, Airaksinen M, Paulin L, Savilahti H: An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction. Genome Res 1999a;9:308-315.
17. Haapa S, Taira S, Heikkinen E, Savilahti H: An efficient and accurate integration of miniMu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications. Nucleic Acids Res 1999b;27:2777-2784.
18. Harshey RM, Getzoff ED, Baldwin DL, Miller JL, Chaconas G: Primary structure of phage mu transposase: homology to mu repressor. Proc Natl Acad Sci USA 1985;82:7676-7680.
19. Izsvák Z, Duda E: 'Star' activity and complete loss of specificity of CeqI endonuclease. Biochem J 1989;258:301-303.
20. Jones DD: Triplet nucleotide removal at random positions in a target gene: the tolerance of TEM-1 beta-lactamase to an amino acid deletion. Nucleic Acids Res 2005;33:e 80.
21. Kuo CF, Zou AH, Jayaram M, Getzoff E, Harshey R: DNA-protein complexes during attachment-site synapsis in Mu DNA transposition. EMBO J 1991;10:1585-1591.
22. Leung P, Teplow D, Harshey R: Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional en hancer. Nature 1989;338:656-658.
23. Mizuuchi M, Baker TA, Mizuuchi K: DNase protection analysis of the stable synaptic complexes involved in Mu transposition. Proc Natl Acad Sci USA 1991;88:9031-9035.
24. Mizuuchi M, Baker TA, Mizuuchi K: Assembly of the active form of the transposase-Mu DNA complex: a critical control point in Mu transposition. Cell 1992;70:303-311.
25. Mizuuchi M, Mizuuchi K: Efficient Mutransposition requires interaction of transposase with a DNA sequence at the Mu operator: implications for regulation. Cell 1989;58:399-408.
26. Nakayama C, Teplow D, Harshey R: Structural domains in phage Mu transposase: identification of the site-specific DNA-binding domain. Proc Natl Acad Sci USA 1987;84:1809-1813.
27. Nordström T, Forsgren A, Riesbeck K: The immunoglobulin D-binding part of the outer membrane protein MID from Moraxella catarrhalis comprises 238 amino acids and a tetrameric structure. J Biol Chem 2002;277:34692-34699.
28. Pasha MK, Dimmock JR, Hollenberg MD, Sharma RK: Enhanced activity of human N-myristoyltransferase by dimethyl sulfoxide and related solvents in the presence of serine/ threonine-containing peptide substrates. Biochem Pharmacol 2002;64:1461-1467.
29. Poussu E, Vihinen M, Paulin L, Savilahti H: Probing the α-complementing domain of E. coli β-galactosidase with use of an insertional pentapeptide mutagenesis strategy based on Mu in vitro DNA transposition. Proteins 2004a;54:681-691.
30. Puglia J, Wang T, Smith-Snyder C, Cote M, Scher M, Pelletier JN, John S, Jonsson CB, Roth MJ: Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins. J Virol 2006;80:9497-9510.
31. Rehan M, Younus H: Effect of organic solvents on the conformation and interaction of catalase and anticatalase antibodies. Int J Biol Macromol 2006;38:289-295.
32. Schumacher S, Clubb R, Cai M, Mizuuchi K, Clore G, Gronenborn A: Solution structure of the Mu end DNA-binding ibeta subdomain of phage Mu transposase: modular DNA recognition by two tethered domains. EMBO J 1997;16:7532-7541.
33. Surette M, Chaconas G: Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the Mu B protein is independent of stable binding of the Mu B protein to DNA. J Biol Chem 1991;266:17306-17313.
34. Surette MG, Buch SJ, Chaconas G: Transpososomes: stable protein-DNA complexes involved in the in vitro transposition of bacte-riophage Mu DNA. Cell 1987;49:253-262.
35. Surette MG, Lavoie BD, Chaconas G: Action at a distance in Mu DNA transposition: an enhancer-like element is the site of action of supercoiling relief activity by integration host factor (IHF). EMBO J 1989;8:3483-3489.
36. Taira S, Tuimala J, Roine E, Nurmiaho-Lassila EL, Savilahti H, Romantschuk M: Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus sub-unit. Mol Microbiol 1999;34:737-744.
37. Tikchonenko T, Karamov E, Zavizion B, Naroditsky B: EcoRI activity: enzyme modification or activation of accompanying endonuclease? Gene 1978;4:195-212.
38. Varrelmann M, Maiss E, Pilot R, Palkovics L: Use of pentapeptide- insertion scanning mutagenesis for functional mapping of the plum pox virus helper component proteinase suppressor of gene silencing. J Gen Virol 2007;88:1005-1015.
39. Watson MA, Chaconas G: Three-site synapsis during Mu DNA transposition: a critical intermediate preceding engagement of the active site. Cell 1996;85:435-445.
40. Wiggers HJ, Cheleski J, Zottis A, Oliva G, Andricopulo AD, Montanari CA: Effects of organic solvents on the enzyme activity of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase in calorimetric assays. Anal Biochem 2007;370:107-114.
41. Wu Z, Chaconas G: Characterization of a region in phage Mu transposase that is involved in interaction with the Mu B protein. J Biol Chem 1994;269:28829-28833.