Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers

Cell Communication and Signaling

Volumn 13, Issue 1, 2015, Pages

  Wehland, Markus ^a   Aleshcheva, Ganna ^a   Schulz, Herbert ^b,c   Saar, Katrin ^b   Hübner, Norbert ^b   Hemmersbach, Ruth ^d   Braun, Markus ^e   Ma, Xiao ^f   Frett, Timo ^d   Warnke, Elisabeth ^a   Riwaldt, Stefan ^a   Pietsch, Jessica ^a   Corydon, Thomas Juhl ^f   Infanger, Manfred ^a   Grimm, Daniela ^f  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c UNIVERSITY OF COLOGNE   (Germany)

^d GERMAN AEROSPACE CENTER DLR   (Germany)

^e UNIVERSITY OF BONN   (Germany)

^f AARHUS UNIVERSITY   (Denmark)

Author keywords

Chondrocytes; Cytokines; Gene expression; Hypergravity; Microgravity; Vibration

Indexed keywords

INTERLEUKIN 6;

ARTICLE; BIRC3 GENE; BMP4 GENE; CARTILAGE CELL; CELL VIABILITY; CONTROLLED STUDY; CYTOKINE RELEASE; DOWN REGULATION; EDN1 GENE; EGF GENE; FGF17 GENE; FLIGHT; GENE EXPRESSION; GENETIC REGULATION; GRAVITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IL6 GENE; IL8 GENE; MICROARRAY ANALYSIS; MICROGRAVITY; MMP10 GENE; MMP3 GENE; PARABOLIC FLIGHT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SUPERNATANT; UPREGULATION; VEGF GENE; VIBRATION; WNT5A GENE; AVIATION; CELL CULTURE; CYTOLOGY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; METABOLISM; WEIGHTLESSNESS;

AVIATION; CELLS, CULTURED; CHONDROCYTES; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; WEIGHTLESSNESS;

EID: 84961545016     PISSN: None     EISSN: 1478811X     Source Type: Journal    
DOI: 10.1186/s12964-015-0095-9     Document Type: Article

References (48)

1. Poole AR, Kojima T, Yasuda T, Mwale F, Kobayashi M, Laverty S. Composition and structure of articular cartilage: a template for tissue repair. Clin Orthop Relat Res. 2001;391(Suppl):S26-33.
2. Aigner T, Stöve J. Collagens-major component of the physiological cartilage matrix, major target of cartilage degeneration, major tool in cartilage repair. Adv Drug Deliv Rev. 2003;55:1569-93.
3. Wiberg C, Klatt AR, Wagener R, Paulsson M, Bateman JF, Heinegård D, et al. Complexes of matrilin-1 and biglycan or decorin connect collagen VI microfibrils to both collagen II and aggrecan. J Biol Chem. 2003;278:37698-704.
4. Tetlow LC, Adlam DJ, Woolley DE. Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes. Arthritis Rheum. 2001;44:585-94.
5. Clements KM, Flannelly JK, Tart J, Brockbank SM, Wardale J, Freeth J, et al. Matrix metalloproteinase 17 is necessary for cartilage aggrecan degradation in an inflammatory environment. Ann Rheum Dis. 2011;70:683-9.
6. Loeser RF. Aging and osteoarthritis: the role of chondrocyte senescence and aging changes in the cartilage matrix. Osteoarthr Cart. 2009;17:971-9.
7. Young AA, Smith MM, Smith SM, Cake MA, Ghosh P, Read RA, et al. Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis. Arthritis Res Ther. 2005;7:R852-61.
8. Alsalameh S, Amin R, Gemba T, Lotz M. Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage. Arthritis Rheum. 2004;50:1522-32.
9. Zayzafoon M, Meyers VE, McDonald JM. Microgravity: the immune response and bone. Immunol Rev. 2005;208:267-80.
10. Greenwald E, Armstrong K, Leon P, Reinberger K. Growing cartilage in space-Understanding the physiological changes from microgravity. [http://chen2820.pbworks.com/w/page/11951455/Growing%20cartilage% 20in%20space].
11. Stamenković V, Keller G, Nesic D, Cogoli A, Grogan SP. Neocartilage formation in 1 g, simulated, and microgravity environments: implications for tissue engineering. Tissue Eng A. 2010;16:1729-36.
12. Pietsch J, Ma X, Wehland M, Aleshcheva G, Schwarzwälder A, Segerer J, et al. Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission. Biomaterials. 2013;34:7694-705.
13. Grosse J, Wehland M, Pietsch J, Schulz H, Saar K, Hübner N, et al. Gravitysensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. FASEB J. 2012;26:5124-40.
14. Ulbrich C, Wehland M, Pietsch J, Aleshcheva G, Wise P, van Loon J, et al. The impact of simulated and real microgravity on bone cells and mesenchymal stem cells. Biomed Res Int. 2014;2014:928507.
15. Ma X, Wehland M, Schulz H, Saar K, Hübner N, Infanger M, et al. Genomic approach to identify factors that drive the formation of three-dimensional structures by EAhy926 endothelial cells. PLoS One. 2013;8:e64402.
16. Ulbrich C, Westphal K, Pietsch J, Winkler HD, Leder A, Bauer J, et al. Characterization of human chondrocytes exposed to simulated microgravity. Cell Physiol Biochem. 2010;25:551-60.
17. Grimm D, Wehland M, Pietsch J, Aleshcheva G, Wise P, van Loon J, et al. Growing tissues in real and simulated microgravity: new methods for tissue Engineering. Tissue Eng B Rev. 2014;20:555-66.
18. Herranz R, Anken R, Boonstra J, Braun M, Christianen PC, de Geest M, et al. Ground-based facilities for simulation of microgravity: organism-specific recommend-dations for their use, and recommended terminology. Astrobiology. 2013;13:1-17.
19. Aleshcheva G, Wehland M, Sahana J, Bauer J, Corydon TJ, Hemmersbach R, et al. Moderate alterations of the cytoskeleton in human chondrocytes after short-term microgravity produced by parabolic flight maneuvers could be prevented by upregulation of BMP-2 and SOX-9. FASEB J.2015. Feb 13. pii: fj.14-268151. [Epub ahead of print].
20. Wehland M, Ma X, Braun M, Hauslage J, Hemmersbach R, Bauer J, et al. The impact of altered gravity and vibration on endothelial cells during a parabolic flight. Cell Physiol Biochem. 2013;31:432-51.
21. Beckmann R, Houben A, Tohidnezhad M, Kweider N, Fragoulis A, Wruck CJ, et al. Mechanical forces induce changes in VEGF and VEGFR-1/sFlt-1 expression in human chondrocytes. Int J Mol Sci. 2014;15:15456-74.
22. Ferrara N. Role of vascular endothelial growth factor in regulation of physiological angiogenesis. Am J Physiol Cell Physiol. 2001;280:C1358-66.
23. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med. 2003;9:669-76.
24. Pufe T, Lemke A, Kurz B, Petersen W, Tillmann B, Grodzinsky AJ, et al. Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor. Am J Pathol. 2004;164:185-92.
25. Pufe T, Kurz B, Petersen W, Varoga D, Mentlein R, Kulow S, et al. The influence of biomechanical parameters on the expression of VEGF and endostatin in the bone and joint system. Ann Anat. 2005;187:461-72.
26. Steinert A, Weber M, Dimmler A, Julius C, Schütze N, Nöth U, et al. Chondrogenic differentiation of mesenchymal progenitor cells encapsulated in ultrahighviscosity alginate. J Orthop Res. 2003;21:1090-7.
27. Reddi AH. Bone morphogenetic proteins: from basic science to clinical applications. J Bone Joint Surg Am. 2001;83A Suppl 1:S1-6.
28. Yuan W, Zhao MD, Yuan FL, Che W, Duan PG, Liu Y, et al. Association of endothelin-1 expression and cartilaginous endplate degeneration in humans. PLoS One. 2013;8:e60062.
29. Saleem M, Qadir MI, Perveen N, Ahmad B, Saleem U, Irshad T, et al. Inhibitors of apoptotic proteins: new targets for anticancer therapy. Chem Biol Drug Des. 2013;82:243-51.
30. Hosseini-Farahabadi S, Geetha-Loganathan P, Fu K, Nimmagadda S, Yang HJ, Richman JM. Dual functions for WNT5A during cartilage development and in disease. Matrix Biol. 2013;32:252-64.
31. Lane Smith R, Trindade MC, Ikenoue T, Mohtai M, Das P, Carter DR, et al. Effects of shear stress on articular chondrocyte metabolism. Biorheology. 2000;37:95-107.
32. Smith RL, Carter DR, Schurman DJ. Pressure and shear differentially alter human articular chondrocyte metabolism: a review. Clin Orthop Relat Res. 2004;427(Suppl):S89-95.
33. Zhang Y, Chee A, Shi P, Wang R, Moss I, Chen EY, et al. Allogeneic articular chondrocyte transplantation downregulates interleukin 8 gene expression in the degenerating rabbit intervertebral disk in vivo. Am J Phys Med Rehabil. 2014. Aug 14. [Epub ahead of print].
34. Ryu JH, Yang S, Shin Y, Rhee J, Chun CH, Chun JS. Interleukin-6 plays an essential role in hypoxia-inducible factor 2α-induced experimental osteoarthritic cartilage destruction in mice. Arthritis Rheum. 2011;63:2732-43.
35. Civinini R, Nistri L, Martini C, Redl B, Ristori G, Innocenti M. Growth factors in the treatment of early osteoarthritis. Clin Cases Miner Bone Metab. 2013;10:26-9.
36. Krejci P, Krakow D, Mekikian PB, Wilcox WR. Fibroblast growth factors 1, 2, 17, and 19 are the predominant FGF ligands expressed in human fetal growth plate cartilage. Pediatr Res. 2007;61:267-72.
37. Blanchard JM. Cyclin A2 transcriptional regulation: modulation of cell cycle control at the G1/S transition by peripheral cues. Biochem Pharmacol. 2000;60:1179-84.
38. Abdollahi T, Robertson NM, Abdollahi A, Litwack G. Inhibition of TRAIL-induced apoptosis by IL-8 is mediated by the p38-MAPK pathway in OVCAR3 cells. Apoptosis. 2005;10:1383-93.
39. Aleshcheva G, Sahana J, Ma X, Hauslage J, Hemmersbach R, Egli M, et al. Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine. PLoS One. 2013;8:e79057.
40. Duke PJ, Daane EL, Montufar-Solis D. Studies of chondrogenesis in rotating systems. J Cell Biochem. 1993;51:274-82.
41. Chavagnac C, Gai F, Gharib T, Mora C. Astrium spaceplane for scientific missions. Acta Astronaut. 2013;92:172-7.
42. Grosse J, Wehland M, Pietsch J, Ma X, Ulbrich C, Schulz H, et al. Short-term weightlessness produced by parabolic flight maneuvers altered gene expression patterns in human endothelial cells. FASEB J. 2012;26:639-55.
43. Ma X, Pietsch J, Wehland M, Schulz H, Saar K, Hübner N, et al. Differential gene expression profile and altered cytokine secretion of thyroid cancer cells in space. FASEB J. 2014;28:813-35.
44. Ulbrich C, Pietsch J, Grosse J, Wehland M, Schulz H, Saar K, et al. Differential gene regulation under altered gravity conditions in follicular thyroid cancer cells: relationship between the extracellular matrix and the cytoskeleton. Cell Physiol Biochem. 2011;28:185-98.
45. Schmidt W. Quickly changing acceleration forces (QCAFs) vibration analysis on the A300 ZERO-G. Microgravity Sci Technol. 2004;15:42-8.
46. Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources. Nat Protoc. 2009;4:44-57.
47. Huang DW, Sherman BT, Lempicki RA. Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res. 2009;37:1-13.
48. Snel B, Lehmann G, Bork P, Huynen MA. STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene. Nucleic Acids Res. 2000;28:3442-4.