Characterization of human chondrocytes exposed to simulated microgravity

Cellular Physiology and Biochemistry

Volumn 25, Issue 4-5, 2010, Pages 551-560

  Ulbrich, Claudia ^a   Westphal, Kriss ^a,d   Pietsch, Jessica ^a   Winkler, Henrik D F ^a   Leder, Annekatrin ^a   Bauer, Johann ^c   Kossmehl, Peter ^a   Grosse, Jirka ^e   Schoenberger, Johann ^e   Infanger, Manfred ^a   Egli, Marcel ^d   Grimm, Daniela ^a,b  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b AARHUS UNIVERSITY   (Denmark)

^c MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^d ETH ZURICH   (Switzerland)

^e UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Apoptosis; Chondrocytes; Cytoskeleton; Extracellular matrix; Growth factors; Microgravity

Indexed keywords

AGGRECAN; BASIC FIBROBLAST GROWTH FACTOR; CASPASE 3; CHONDROITIN SULFATE; COLLAGEN TYPE 1; COLLAGEN TYPE 10; COLLAGEN TYPE 2; COLLAGEN TYPE 4; CYTOSKELETON PROTEIN; FAS ANTIGEN; LAMININ; PROTEIN BCL 2; PROTEIN P53; VASCULOTROPIN;

APOPTOSIS; ARTICLE; CARTILAGE CELL; CELL AGGREGATION; CONTROLLED STUDY; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; MACHINE; MICROGRAVITY; PRIORITY JOURNAL; SIMULATION; TISSUE ENGINEERING; TISSUE REGENERATION;

EID: 77949929784     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000303059     Document Type: Article

References (52)

1. Muir H: The chondrocyte, architecture of cartilage-Biomechanics, structure, function and molecular-biology of cartilage matrix macromolecules. Bioessays 1995;17:1039-1048.
2. Verzijl N, Bank RA, TeKoppele JM, DeGroot J: AGEing and osteoarthritis: a different perspective. Curr Opinion Rheumatol 2003;15:616-622.
3. Suh JKF, Matthew HWT: Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 2000;21:2589-2598.
4. Marijnissen WJCM, van Osch GJVM, Aigner J, Verwoerd-Verhoef HL, Verhaar JAN: Tissue-engineered cartilage using serially passaged articular chondrocytes. Chondrocytes in alginate, combined in vivo with a synthetic (E210) or biologic biodegradable carrier (DBM). Biomaterials 2000;21:571-580.
5. Brun P, Abatangelo G, Radice M, Zacchi V, Guidolin D, Gordini DD, Cortivo R: Chondrocyte aggregation and reorganization into three-dimensional scaffolds. J Biomed Mat Res 1999;46:337-346.
6. Schulz RM, Bader A: Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur Biophys J 2007;36:539-568.
7. Pound JC, Green DW, Roach HI, Mann S, Oreffo RO: An ex vivo model for chondrogenesis and osteogenesis. Biomaterials 2007;28:2839-2849.
8. Hutmacher DW: Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000;21:2529-2543.
9. Grimm D, Bauer J, Kossmehl P, Shakibaei M, Schönberger J, Pickenhahn H, Schulze-Tanzil G, Vetter R, Eilles C, Paul M, Cogoli A: Simulated microgravity alters differentiation ad increases apoptosis in human follicular thyroid carcinoma cells. FASEB J 2002;16:604-614.
10. Infanger M, Kossmehl P, Shakibaei M, Baatout S, Witzing A, Grosse J, Bauer J, Cogoli A, Faramarzi S, Derradji H, Neefs M, Paul M, Grimm D: Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: impact of vascular endothelial growth factor. Apoptosis 2006;11:749-764.
11. Smith YC, Grande KK, Rasmussen SB, O'Brien AD: Novel three-dimensional organoid model for evaluation of the interaction of uropathogenic Escherichia coli with terminally differentiated human urothelial cells Infect Immun 2006;74:750-757.
12. Cameron DF, Hushen JJ, Colina L, Mallery J, Willing A, Sanberg PR, Saporta S: Formation and structure of transplantable tissue constructs generated in simulated microgravity from sertoli cells and neuron precursors Cell Transplant 2004;13:755-763.
13. Freed LE, Langer R, Martin I, Pellis NR, Vunjak-Novakovic G: Tissue engineering of cartilage in space. Proc Natl Acad Sci USA 1997;94:13885-13890.
14. Hsu SH, Kuo CC, Yen HJ, Whu SW, Tsai CL: The effect of two different bioreactors on the neocartilage formation in type II collagen modified polyester scaffolds seeded with chondrocytes. Artifical Organs 2005:29:467-474.
15. Klement BJ, Spooner DS: Mineralization and growth of cultured embryonic skeletal tissue in microgravity. Bone 1999;24:349-359.
16. Duke PJ, Daane EL, Montufar-Solis D: Studies of chondrogenesis in rotating systems. J Cell Biochem 1993;51:274-282.
17. Montufar-Solis D, Duke PJ: Gravitational changes affect tibial growth plates according to Hert's curve. Aviation Space Environ Med 1999;70:245-249.
18. Hoson T, Kamisaka S, Masuda Y, Yamashita M: Changes in plant growth processes under microgravity conditions simulated by a three-dimensional clinostat. Bot Mag 1992;105:53-70.
19. van Loon JJWA: Some history and use of the random positioning machine, RPM, in gravity relayed research. Adv Space Res 2007;39:1161-1165.
20. Kossmehl P, Kurth E, Faramarzi S, Habighorst B, Shakibaei M, Wehland M, Kreutz R, Infanger M, J Danser AH, Grosse J, Paul M, Grimm D: Mechanisms of apoptosis after ischemia and reperfusion: role of the renin-angiotensin system. Apoptosis 2006;11:347-358.
21. Grimm D, Jabusch HC, Kossmehl P, Huber M, Fredersdorf S, Griese DP, Krämer BK, Kromer EP: Experimental diabetes and left ventricular hypertrophy: effects of beta-receptor blockade. Cardiovasc Pathol 2002;11:229-237.
22. Riecke K, Grimm D, Shakibaei M, Kossmehl P, Schulze-Tanzil G, Paul M, Stahlmann R: Low doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin increase transforming growth factor beta and cause myocardial fibrosis in marmosets (Callithrix jacchus). Arch Toxicol 2002;76:360-366.
23. Rothermund L, Kreutz R, Kossmehl P, Fredersdorf S, Shakibaei M, Schulze-Tanzil G, Paul M, Grimm D: Early onset of chondroitin sulfate and osteopontin expression in angiotensin II-dependent left ventricular hypertrophy. Am J Hypertens 2002;15:644-652.
24. Gehrmann M, Marienhagen J, Eichholtz-Wirth H, Fritz E, Ellwart J, Jäättelä M, Zilch T, Multhoff G: Dual function of membrane-bound heat shock protein 70 (Hsp70), Bag-4, and Hsp40: protection against radiation-induced effects and target structure for natural killer cells. Cell Death Differ 2005;12:38-51.
25. Infanger M, Ulbrich C, Baatout S, Wehland M, Kreutz R, Bauer J, Grosse J, Vadrucci S, Cogoli A, Derradji H, Neefs M, Küsters S, Spain M, Paul M, Grimm D: Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells. J Cell Biochem 2007;101:1439-1455.
26. Ulbrich C, Westphal K, Baatout S, Wehland M, Bauer J, Flick B, Infanger M, Kreutz R, Vadrucci S, Egli M, Cogoli A, Derradji H, Pietsch J, Paul M, Grimm D: Effects of basic Fibroblast Growth Factor on endothelial cells under conditions of simulated microgravity. J Cell Biochem 2008;104:1324-1341.
27. Grosse J, Grimm D, Westphal K, Ulbrich C, Moosbauer J, Pohl F, Koelbl O, Infanger M, Eilles C, Schoenberger J: Radiolabeled annexin V for imaging apoptosis in radiated human follicular thyroid carcinomas-is an individualized protocol necessary? Nucl Med Biol 2009;36:89-98.
28. Conza N, Mainil-Varlet P, Rieser F, Kraemer J, Bittmann P, Huijser R, van den Bergh L, Cogoli A: Tissue engineering in space. J Gravit Physiol 2001; 8: P17-20.
29. Poole AR, Pidoux I, Rosenberg L: Role of Proteoglycans in Endochondral Ossification: Immunofluorescent Localization of Link Protein and Proteoglycan Monomer in Bovine Fetal Epiphyseal Growth Plate J Cell Biol 1982;92:249-260.
30. Lo MY, Kim HAT: Chondrocyte apoptosis induced by hydrogen peroxide requires caspase activation but not mitochondrial pore transition. J. Orthopaedic Res. 2004;22:1120-1125.
31. Chen JH, Cao JL, Chu YL, Wang ZL, Yang ZT, Wang HL: T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes. J Zhejiang Univ-Sci B 2008;9:455-463.
32. Whiteman M, Armstrong JS, Cheung NS, Siau JL, Rose P, Schantz JT, Jones DP, Halliwell B: Peroxynitrite mediates calcium-dependent mitochondrial dysfunction and cell death via activation of calpains. FASEB J 2004;18:1395-1397.
33. Uva BM, Masini MA, Sturla M, Buzzone F, Giuliani M, Tagliaferro G, Strollo F: Microgravity-induced apoptosis in cultured glial cells. Eur J Histochem 2002;46:209-214.
34. Dürr J, Lammi P, Goodman SL, Aigner T, von der Mark K: Identification and immunolocalization of laminin in cartilage. Exp Cell Res 1996;222:225-333.
35. Kvist AJ, Nystrom A, Hultenby K, Sasaki T, Talts JF, Aspberg A: The major basement membrane components localize to the chondrocyte pericellular matrix-A cartilage basement membrane equivalent? Matrix Biol 2008;27:22-33.
36. Frondoza C, Sohrabi A, Hungerford D: Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture. Biomaterials 1996;17:879-1188
37. Mundlos S, Zabel B: Developmental expression of human cartilage matrix proteins. Dev Dyn 1994;199:241-1152
38. Schnabel M, Marlovits S, Eckhoff G, Fichtel L, Gotzen L, Vécsei V, Schlegel J: Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthritis Cartilage 2002;10:62-70.
39. Valle PL, Iwamoto M, Fanning P, Pacifici M, Olson BR: Multiple negative elements in a gene that codes for an extracellular-matrix protein, collagen x, restrict expression to hypertrophic chondrocytes. J Cell Biol 1993;121:1173-1179.
40. Vornehm SI, Dudhia J, VonderMark K, Aigner T: Expression of collagen types IX and XI and other major cartilage matrix components by human fetal chondrocytes in vivo. Matrix Biol 1996;15:91-98.
41. Lemons ML, Sandy JD, Anderson DK, Howland DR: Intact aggrecan and chondroitin sulfate-depleted aggrecan core glycoprotein inhibit axon growth in the adult rat spinal cord. Exp Neurol 2003;184:981-990.
42. Fioravanti A, Collodel G: In vitro effects of chondroitin sulfate. Adv Pharmacol 2006;53:449-465.
43. Gerber HP, Vu T, Ryan A, Kowalski J, Werb Z, Ferrara N: VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during enchondral bone formation. Nature Med 1999;5:623-638.
44. Horner A, Bishop NJ, Bord S, Beeton C, Kelsall AW, Coleman N, Compston JE: Immunolocalisation of vascular endothelial growth factor (VEGF) in human neonatal growth plate cartilage. J Anat 1999;194:519-524.
45. Infanger M, Schmidt O, Kossmehl P, Grad S, Ertel W, Grimm D: Vascular endothe-lial growth factor serum level is strongly enhanced after burn injury and correlated with local and general tissue edema. Burns 2004;30:305-311.
46. Harper J, Klagsbrun M: Cartilage to bone-angiogenesis leads the way. Nature Med 1999;5: 623-628.
47. Carlevaro MF, Cermelli S, Cancedda R, Cancedda FD: Vascular endothelial growth factor (VEGF) in cartilage neovascularization and chondrocyte differentiation: auto-paracrine role during endochondral bone formation. J Cell Sci 2000;113:59-69.
48. Schmal H, Zwingmann J, Fehrenbacher M, Finkenzeller G, Stark GB, Südkamp NP, Hartl D, Mehlhorn AT: bFGF influences human articular chondrocyte differentiation. Cytotherapy 2007;9:184-193.
49. Mehrishi JN, Bauer J: Electrophoresis of cells and the biological relevance of surface charge. Electrophoresis 2002;23:1984-1994.
50. Grimm D, Infanger M, Ulbrich C, Pietsch J, Kossmehl P, Vadrucci S, Baatout S, Flick B, Paul M, Bauer J: A delayed type of three-dimensional growth of human endothelial cells under simulated weightlessness. Tissue Eng Part A 2009;15: 2267-2275.
51. Uva BM, Masini MA, Sturla M, Prato P, Passalacqua M, Giuliani M, Tagliafierro G, Strollo F: Clinorotation-induced weightlessness influences the cytoskeleton of glial cells in culture. Brain Res 2002;934:132-139.
52. Meloni MA, Galleri G, Pippia P, Cogoli-Greuter M: Cytoskeleton changes and impaired motility of monocytes at modelled low gravity. Protoplasma 2006;229:243-249.