Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi

Nucleic Acids Research

Volumn 43, Issue 7, 2015, Pages e45-

  Kaulich, Manuel ^a   Lee, Yeon J ^a   Lönn, Peter ^a   Springer, Aaron D ^a   Meade, Bryan R ^a   Dowdy, Steven F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENO ASSOCIATED VIRUS; ALLELE; ARTICLE; CDK1 GENE; CDK2 GENE; CELL CYCLE ARREST; CONTROLLED STUDY; CRISPR CAS SYSTEM; GENE; GENE CONTROL; GENE EXPRESSION; GENE LOCUS; GENE MUTATION; GENE SILENCING; GENE TARGETING; GENETIC ENGINEERING; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DEPENDOPARVOVIRUS; GENETIC RECOMBINATION; GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ADENO-ASSOCIATED VIRUS; MAMMALIA;

CDK1 PROTEIN, HUMAN; CDK2 PROTEIN, HUMAN; CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE 2; PRIMER DNA;

ALLELES; BASE SEQUENCE; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CYCLIN-DEPENDENT KINASE 2; CYCLIN-DEPENDENT KINASES; DEPENDOVIRUS; DNA PRIMERS; GENE KNOCKDOWN TECHNIQUES; HUMANS; MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; RNA INTERFERENCE;

EID: 84961525313     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gku1403     Document Type: Article

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