Human proximal tubule cells form functional microtissues

Pflugers Archiv European Journal of Physiology

Volumn 468, Issue 4, 2016, Pages 739-750

  Prange, Jenny A ^a   Bieri, Manuela ^a   Segerer, Stephan ^b   Burger, Charlotte ^a   Kaech, Andres ^a   Moritz, Wolfgang ^c   Devuyst, Olivier ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

^c InSphero AG   (Switzerland)

Author keywords

Albumin; Drug toxicity; Endocytosis; Epithelial cells; Primary culture

Indexed keywords

ALBUMIN; CADMIUM; GENTAMICIN; CONDITIONED MEDIUM;

ARTICLE; CELL CONTACT; CELL DEDIFFERENTIATION; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRUCTURE; COCULTURE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENDOCYTOSIS; ENDOSOME; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMORTALIZED CELL LINE; KIDNEY PARENCHYMA; KIDNEY PROXIMAL TUBULE; KIDNEY TUBULE CELL; LYSOSOME; MICROVILLUS; PRIORITY JOURNAL; TIGHT JUNCTION; TISSUE CULTURE; TISSUE SPECIFICITY; CELL CULTURE; CELL LINE; CONDITIONED MEDIUM; CYTOLOGY; DRUG EFFECTS; METABOLISM; PHARMACOLOGY; PHYSIOLOGY; PRIMARY CELL CULTURE; PROCEDURES; TISSUE CULTURE TECHNIQUE;

CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CELLS, CULTURED; COCULTURE TECHNIQUES; CULTURE MEDIA, CONDITIONED; EPITHELIAL CELLS; FIBROBLASTS; HUMANS; KIDNEY TUBULES, PROXIMAL; PRIMARY CELL CULTURE; TISSUE CULTURE TECHNIQUES;

EID: 84961111539     PISSN: 00316768     EISSN: 14322013     Source Type: Journal    
DOI: 10.1007/s00424-015-1771-8     Document Type: Article

References (39)

1. Abbott A (2003) Cell culture: biology’s new dimension. Nature 424:870–872
2. Ahn DW, Chung JM, Kim JY et al (2005) Inhibition of renal Na+/H+ exchange in cadmium-intoxicated rats. Toxicol Appl Pharmacol 204:91–98
3. Ali BH (1995) Gentamicin nephrotoxicity in humans and animals: some recent research. Gen Pharmacol 26:1477–1487
4. Araki M, Takano T, Uemonsa T et al (2002) Epithelia-mesenchyme interaction plays an essential role in transdifferentiation of retinal pigment epithelium of silver mutant quail: localization of FGF and related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation. Dev Biol 244:358–371
5. Boehnke K, Mirancea N, Pavesio A et al (2007) Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur J Cell Biol 86:731–746
6. Christensen EI, Birn H, Storm T et al (2012) Endocytic receptors in the renal proximal tubule. Physiology (Bethesda) 27:223–236
7. Christensen EI, Devuyst O, Dom G et al (2003) Loss of chloride channel ClC-5 impairs endocytosis by defective trafficking of megalin and cubilin in kidney proximal tubules. Proc Natl Acad Sci U S A 100:8472–8477
8. Devuyst O, Luciani A (2015) Chloride transporters and receptor-mediated endocytosis in the renal proximal tubule. J Physiol 593:4151–4164
9. Drewitz M, Helbling M, Fried N et al (2011) Towards automated production and drug sensitivity testing using scaffold-free spherical tumor microtissues. Biotechnol J 6:1488–1496
10. Eckardt KU, Coresh J, Devuyst O et al (2013) Evolving importance of kidney disease: from subspecialty to global health burden. Lancet 382:158–69
11. El Ghalbzouri A, Ponec M (2004) Diffusible factors released by fibroblasts support epidermal morphogenesis and deposition of basement membrane components. Wound Repair Regen 12:359–367
12. Gena P, Calamita G, Guggino WB (2010) Cadmium impairs albumin reabsorption by down-regulating megalin and ClC5 channels in renal proximal tubule cells. Environ Health Perspect 118:1551–1556
13. Giles RH, Ajzenberg H, Jackson PK (2014) 3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders. Nat Protoc 9:2725–2731. doi:10.1038/nprot.2014.181
14. Kalluri R, Zeisberg M (2006) Fibroblasts in cancer. Nat Rev Cancer 6:392–401
15. Kelm JM, Moritz W, Schmidt D et al (2007) In vitro vascularization of human connective microtissues. Methods Mol Med 140:153–166
16. Kobayashi K, Nomoto Y, Suzuki T et al (2006) Effect of fibroblasts on tracheal epithelial regeneration in vitro. Tissue Eng 12:2619–2628
17. Lancaster MA, Knoblich JA (2014) Organogenesis in a dish: modeling development and disease using organoid technologies. Science 345:1247125
18. Lima WR, Parreira KS, Devuyst O et al (2010) ZONAB promotes proliferation and represses differentiation of proximal tubule epithelial cells. J Am Soc Nephrol 21:478–488
19. Lote CJ (2012) In: principles of renal physiology, 5th edn. Springer, New York, p 25
20. Meli L, Barbosa HS, Hickey AM et al (2014) Three dimensional cellular microarray platform for human neural stem cell differentiation and toxicology. Stem Cell Res 13:36–47
21. Monteil C, Leclere C, Fillastre JP et al (1993) Characterization of gentamicin-induced dysfunctions in vitro: the use of optimized primary cultures of rabbit proximal tubule cells. Ren Fail 15:475–483
22. Pampaloni F, Reynaud EG, Stelzer EH (2007) The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 8:839–845
23. Pampaloni F, Stelzer EH, Masotti A (2009) Three-dimensional tissue models for drug discovery and toxicology. Recent Pat Biotechnol 3:103–117
24. Pfaller W, Gstraunthaler G (1998) Nephrotoxicity testing in vitro—what we know and what we need to know. Environ Health Perspect 106(Suppl 2):559–569
25. Raggi C, Fujiwara K, Leal T et al (2011) Decreased renal accumulation of aminoglycoside reflects defective receptor-mediated endocytosis in cystic fibrosis and Dent’s disease. Pflugers Arch 462:851–860
26. Raggi C, Luciani A, Nevo N et al (2014) Dedifferentiation and aberrations of the endolysosomal compartment characterize the early stage of nephropathic cystinosis. Hum Mol Genet 23:2266–2278
27. Reed AA, Loh NY, Terryn S et al (2010) CLC-5 and KIF3B interact to facilitate CLC-5 plasma membrane expression, endocytosis, and microtubular transport: relevance to pathophysiology of Dent’s disease. Am J Physiol Renal Physiol 298:F365–380
28. Regec AL, Trifillis AL, Trump BF (1986) The effect of gentamicin on human renal proximal tubular cells. Toxicol Pathol 14:238–241
29. Schmidt SL, Carter LL (1990) ATP is required for receptor-mediated endocytosis in intact cells. JCB 111:2307–2318
30. Silverblatt FJ, Kuehn C (1979) Autoradiography of gentamicin uptake by the rat proximal tubule cell. Kidney Int 15:335–345
31. Takasato M, Er PX, Becroft M et al (2014) Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. Nat Cell Biol 16:118–126
32. Takasato M, Er PX, Chiu HS et al (2015) Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature 526:564–568
33. Tanaka K, Terryn S, Geffers L et al (2010) The transcription factor HNF1α regulates expression of chloride-proton exchanger ClC-5 in the renal proximal tubule. Am J Physiol Renal Physiol 299:F1339–F1347
34. Terryn S, Jouret F, Vandenabeele F et al (2007) A primary culture of mouse proximal tubular cells, established on collagen-coated membranes. Am J Physiol Renal Physiol 293:F476–485
35. Thevenod F (2003) Nephrotoxicity and the proximal tubule. Insights from cadmium. Nephron Physiol 93:p87–93
36. van Meerloo J, Kaspers GJ, Cloos J (2011) Cell sensitivity assays: the MTT assay. Methods Mol Biol 731:237–245
37. Vandewalle A, Farman N, Morin JP et al (1981) Gentamicin incorporation along the nephron: autoradiographic study on isolated tubules. Kidney Int 19:529–539
38. Wohlwend A, Montesano R, Vassalli JD, Orci L (1985) LLC-PK1 cysts: a model for the study of epithelial polarity. J Cell Physiol 125:533–539
39. Zalups RK, Ahmad S (2003) Molecular handling of cadmium in transporting epithelia. Toxicol Appl Pharmacol 186:163–188