A primary culture of mouse proximal tubular cells, established on collagen-coated membranes

American Journal of Physiology - Renal Physiology

Volumn 293, Issue 2, 2007, Pages

  Terryn, Sara ^a   Jouret, François ^c   Vandenabeele, Frank ^a,b   Smolders, Inge ^a,b   Moreels, Marjan ^a,b   Devuyst, Olivier ^b   Steels, Paul ^a,b   Van Kerkhove, Emmy ^a  

^a HASSELT UNIVERSITY   (Belgium)

^b BIOMED Research Institute   (Belgium)

^c UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

Aquaporin; Electrophysiological characteristics; Phloridzin; Receptor mediated endocytosis; Sodium dependent glucose transport; Ussing chamber

Indexed keywords

ALBUMIN; ALKALINE PHOSPHATASE; AQUAPORIN; COLLAGEN; COLLAGENASE; FLUORESCEIN ISOTHIOCYANATE; GAMMA GLUTAMYLTRANSFERASE; PHLORIZIN; SODIUM ION;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CONTROLLED STUDY; ELECTROPHYSIOLOGY; GLUCOSE TRANSPORT; IMMUNOBLOTTING; IMMUNODETECTION; IMMUNOHISTOCHEMISTRY; KIDNEY CELL; KIDNEY CORTEX; KIDNEY PROXIMAL TUBULE; MEMBRANE FILTER; MOUSE; NONHUMAN; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY;

EID: 34548439530     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.00363.2006     Document Type: Article

References (35)

1. Agre P, King LS, Yasui M, Guggino WB, Ottersen OP, Fujiyoshi Y, Engel A, Nielsen S. Aquaporin water channels - from atomic structure to clinical medicine. J Physiol 542: 3-16, 2002.
2. Bell CL, Tenenhouse HS, Scriver CR. Isolation and culture of murine renal proximal tubule cells: a system to study solute transport in mutants. Ann NY Acad Sci 456: 398-400, 1985.
3. Bello-Reuss E, Weber MR. Electrophysiological studies on primary cultures of proximal tubule cells. Am J Physiol Renal Fluid Electrolyte Physiol 251: F490-F498, 1986.
4. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976.
5. Brown D, Hirsch S, Gluck S. Localization of a proton-pumping ATPase in rat kidney. J Clin Invest 82: 2114-2126, 1988.
6. Christensen EI, Birn H. Megalin and cubilin: multifunctional endocytic receptors. Nat Rev Mol Cell Biol 3: 256-266, 2002.
7. Christensen EI, Devuyst O, Dom G, Nielsen R, Van der Smissen P, Verroust P, Leruth M, Guggino WB, Courtoy PJ. Loss of chloride channel ClC-5 impairs endocytosis by defective trafficking of megalin and cubilin in kidney proximal tubules. Proc Natl Acad Sci USA 100: 8472-8477, 2003.
8. Cummings BS, Lasker JM, Lash LH. Expression of glutathione-dependent enzymes and cytochrome P-450s in freshly isolated and primary cultures of proximal tubular cells from human kidney. J Pharmacol Exp Ther 293: 677-685, 2000.
9. Del Valle PL, Trifillis A, Ruegg CE, Kane AS. Characterization of glucose transport by cultured rabbit kidney proximal convoluted and proximal straight tubule cells. In Vitro Cell Dev Biol Anim 38: 218 -227, 2002.
10. Devuyst O, Christie PT, Courtoy PJ, Beauwens R, Thakker RV. Intra-renal and subcellular distribution of the human chloride channel, CLC-5, reveals a pathophysiological basis for Dent's disease. Hum Mol Genet 8: 247-257, 1999.
11. Elliget KA, Trump BF. Primary cultures of normal rat kidney proximal tubule epithelial cells for studies of renal cell injury. In Vitro Cell Dev Biol 27A: 739-748, 1991.
12. Gekle M, Mildenberger S, Freudinger R, Silbernagl S. Functional characterization of albumin binding to the apical membrane of OK cells. Am J Physiol Renal Fluid Electrolyte Physiol 271: F286-F291, 1996.
13. Glossmann H, Neville DM. gamma-Glutamyltransferase in kidney brush border membranes. FEBS Lett 19: 340-344, 1972.
14. Hanigan MH, Gallagher BC, Townsend DM, Gabarra V. Gamma-Glutamyl transpeptidase accelerates tumor growth and increases the resistance of tumors to cisplatin in vivo. Carcinogenesis 20: 553-559, 1999.
15. Jans F, Vandenabeele F, Helbert M, Lambrichts I, Ameloot M, Steels P. A simple method for obtaining functionally and morphologically intact primary cultures of the medullary thick ascending limb of Henle's loop (MTAL) from rabbit kidneys. Pflügers Arch 440: 643-651, 2000.
16. Jouret F, Igarashi T, Gofflot F, Wilson PD, Karet FE, Thakker RV, Devuyst O. Comparative ontogeny, processing, and segmental distribution of the renal chloride channel, ClC-5. Kidney Int 65: 198 -208, 2004.
17. Lazzara MJ, Deen WM. Model of albumin reabsorption in the proximal tubule. Am J Physiol Renal Physiol 292: F340-F349, 2007.
18. Loffing J, Vallon V, Loffing-Cueni D, Aregger F, Richter K, Pietri L, Bloch-Faure M, Hoenderop JG, Shull GE, Meneton P, Kaissling B. Altered renal distal tubule structure and renal Na(+) and Ca(2+) handling in a mouse model for Gitelman's syndrome. J Am Soc Nephrol 15: 2276-2288, 2004.
19. Pan J, Wang Q, Snell WJ. Cilium-generated signaling and cilia-related disorders. Lab Invest 85: 452-463, 2005.
20. Park CH, Maack T. Albumin absorption and catabolism by isolated perfused proximal convoluted tubules of the rabbit. J Clin Invest 73: 767-777, 1984.
21. Rodeheaver DP, Aleo MD, Schnellmann RG. Differences in enzymatic and mechanical isolated rabbit renal proximal tubules: comparison in long-term incubation. In Vitro Cell Dev Biol 26: 898-904, 1990.
22. +-ATPase. Am J Physiol Renal Physiol 277: F957-F965, 1999.
23. Sakhrani LM, Badie-Dezfooly B, Trizna W, Mikhail N, Lowe AG, Taub M, Fine LG. Transport and metabolism of glucose by renal proximal tubular cells in primary culture. Am J Physiol Renal Fluid Electrolyte Physiol 246: F757-F764, 1984.
24. Schaaf GJ, de Groene EM, Maas RF, Commandeur JN, Fink-Gremmels J. Characterization of biotransformation enzyme activities in primary rat proximal tubular cells. Chem Biol Interact 134: 167-190, 2001.
25. Schwegler JS, Heppelmann B, Mildenberger S, Silbernagl S. Receptor-mediated endocytosis of albumin in cultured opossum kidney cells: a model for proximal tubular protein reabsorption. Pflügers Arch 418: 383-392, 1991.
26. Sheridan AM, Schwartz JH, Kroshian VM, Tercyak AM, Laraia J, Masino S, Lieberthal W. Renal mouse proximal tubular cells are more susceptible than MDCK cells to chemical anoxia. Am J Physiol Renal Fluid Electrolyte Physiol 265: F342-F350, 1993.
27. Spring KR, Hope A. Fluid transport and the dimensions of cells and interspaces of living Necturus gallbladder. J Gen Physiol 73: 287-305, 1979.
28. Taub ML, Yang IS, Wang Y. Primary rabbit kidney proximal tubule cell cultures maintain differentiated functions when cultured in a hormonally defined serum-free medium. In Vitro Cell Dev Biol 25: 770-775, 1989.
29. Van der Biest I, Nouwen EJ, Van Dromme SA, De Broe ME. Characterization of pure proximal and heterogeneous distal human tubular cells in culture. Kidney Int 45: 85-94, 1994.
30. Van Driessche W, De Vos R, Jans D, Simaels J, De Smet P, Raskin G. Transepithelial capacitance decrease reveals closure of lateral interspace in A6 epithelia. Pflügers Arch 437: 680-690, 1999.
31. Wagner CA, Lukewille U, Valles P, Breton S, Brown D, Giebisch GH, Geibel JP. A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse. Pflügers Arch 446: 623- 632, 2003.
32. Walter K, Schütt C. Acid and alkaline phosphatase in serum (two-point method). In: Methods of Enzymatic Analysis, edited by Bergmeyer HU. New York: Academic, 1974, p. 856-864.
33. Wang SS, Devuyst O, Courtoy PJ, Wang XT, Wang H, Wang Y, Thakker RV, Guggino S, Guggino WB. Mice lacking renal chloride channel, CLC-5, are a model for Dent's disease, a nephrolithiasis disorder associated with defective receptor-mediated endocytosis. Hum Mol Genet 9: 2937-2945, 2000.
34. Weinstein AM. Sodium and chloride transport: Proximal nephron. In: The Kidney: Physiology and Pathophysiology, edited by Seldin DW and Giebisch G. Philadelphia: Lippincott Williams & Wilkins, 2000, p. 1287-1331.
35. +-glucose cotransporters. Am J Physiol Renal Physiol 280: F10-F18, 2001.