3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders

Nature Protocols

Volumn 9, Issue 12, 2014, Pages 2725-2731

  Giles, Rachel H ^a   Ajzenberg, Henry ^a   Jackson, Peter K ^b,c  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b GENENTECH INC   (United States)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MATRIGEL; SMALL INTERFERING RNA; COLLAGEN; DRUG COMBINATION; LAMININ; PROTEOGLYCAN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL POLARITY; CILIARY DYSKINESIA; CONTROLLED STUDY; EX VIVO STUDY; GENE DOSAGE; GENE SILENCING; GENETIC ANALYSIS; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; INNER MEDULLARY COLLECTING DUCT CELL; KIDNEY CELL; KIDNEY COLLECTING TUBULE; KIDNEY DISEASE; KIDNEY EPITHELIUM; KIDNEY INNER MEDULLA; MOUSE; NEPHRONOPHTHISIS; NONHUMAN; PRIORITY JOURNAL; SPHEROID CELL; THREE DIMENSIONAL IMAGING; ANIMAL; CULTURE TECHNIQUE; CYTOLOGY; DRUG COMBINATION; EPITHELIUM CELL; GENE INACTIVATION; IMMUNOHISTOCHEMISTRY; KIDNEY POLYCYSTIC DISEASE; MULTICELLULAR SPHEROID; PATHOLOGY; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; COLLAGEN; DRUG COMBINATIONS; EPITHELIAL CELLS; GENE DOSAGE; GENE KNOCKOUT TECHNIQUES; HUMANS; IMAGING, THREE-DIMENSIONAL; IMMUNOHISTOCHEMISTRY; KIDNEY DISEASES; KIDNEY DISEASES, CYSTIC; LAMININ; MICE; PROTEOGLYCANS; RNA, SMALL INTERFERING; SPHEROIDS, CELLULAR; TRANSFECTION;

EID: 84941639441     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.181     Document Type: Article

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