Selective disruption of disulphide bonds lowered activation energy and improved catalytic efficiency in TALipB from Trichosporon asahii MSR54: MD simulations revealed flexible lid and extended substrate binding area in the mutant

Biochemical and Biophysical Research Communications

Volumn 472, Issue 1, 2016, Pages 223-230

  Singh, Yogesh ^a   Gupta, Namita ^a   Verma, Ved Vrat ^a   Goel, Manisha ^a   Gupta, Rani ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Cysteine; Disulfide bond; Lid; Lipase; Molecular dynamics; Trichosporon asahii

Indexed keywords

1 PHENYLETHANOL; ALANINE; CYSTEINE; ESTER; FUNGAL ENZYME; TALIPB ENZYME; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; DISULFIDE; FUNGAL PROTEIN; RECOMBINANT PROTEIN;

ALPHA HELIX; ARTICLE; BETA SHEET; CATALYTIC EFFICIENCY; CIRCULAR DICHROISM; COMPUTER MODEL; DISULFIDE BOND; ENANTIOSELECTIVITY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME STABILITY; ESTERIFICATION; GENE DISRUPTION; MOLECULAR DYNAMICS; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; ROTATION; SURFACE AREA; TEMPERATURE; TRICHOSPORON ASAHII; ALGORITHM; AMINO ACID SUBSTITUTION; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; FUNGAL GENE; GENETICS; METABOLISM; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; TRICHOSPORON;

ALGORITHMS; AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; DISULFIDES; ENZYME ACTIVATION; ENZYME STABILITY; FUNGAL PROTEINS; GENES, FUNGAL; LIPASE; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; TRICHOSPORON;

EID: 84960416146     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2016.01.189     Document Type: Article

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