Alteration of lipase chain length specificity in the hydrolysis of esters by random mutagenesis

Biotechnology and Bioengineering

Volumn 73, Issue 6, 2001, Pages 433-441

  Gaskin, Duncan J H ^a   Romojaro, Ana ^a   Turner, Nigel A ^a   Jenkins, John ^a   Vulfson, Evgeny N ^a  

^a NORWICH RESEARCH PARK   (United Kingdom)

Author keywords

Ester; Hydrolysis; Random mutagenesis; Recombinant lipase; Selectivity

Indexed keywords

DNA; ENZYMES; ESCHERICHIA COLI; ESTERS; MUTAGENESIS; YEAST;

MUTANTS;

HYDROLYSIS;

ESTER; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; ARTICLE; DNA SEQUENCE; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC ANALYSIS; HYDROLYSIS; MUTAGENESIS; PICHIA PASTORIS; PROTEIN EXPRESSION; RHIZOMUCOR MIEHEI;

AMINO ACID SUBSTITUTION; BINDING SITES; BUTYRIC ACID; CHROMATOGRAPHY, AGAROSE; ESCHERICHIA COLI; ESTERS; HYDROLYSIS; LIPASE; MUTAGENESIS; PHENYLALANINE; PICHIA; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RHIZOMUCOR; SUBSTRATE SPECIFICITY;

PICHIA PASTORIS; RHIZOMUCOR MIEHEI;

EID: 0035919135     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1077     Document Type: Article

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