Transcriptome-enabled discovery and functional characterization of enzymes related to (2S)-pinocembrin biosynthesis from Ornithogalum caudatum and their application for metabolic engineering

Microbial Cell Factories

Volumn 15, Issue 1, 2016, Pages

  Guo, Lei ^a   Chen, Xi ^a,b   Li, Li Na ^a   Tang, Wei ^b   Pan, Yi Ting ^c   Kong, Jian Qiang ^a  

^a BEIJING HOSPITAL   (China)

^b WUHAN UNIVERSITY   (China)

^c BEIJING INSTITUTE OF PETROCHEMICAL TECHNOLOGY   (China)

Author keywords

(2S) Pinocembrin; 4 Coumarate:coenzyme A ligase; Chalcone isomerase; Chalcone synthase; Metabolic engineering; Ornithogalum caudatum

Indexed keywords

BENZOIC ACID; CAFFEIC ACID; CERULENIN; CHALCONE ISOMERASE; CHALCONE SYNTHASE; CINNAMIC ACID; COENZYME A LIGASE; FERULIC ACID; ISOMERASE; MALONYL COENZYME A; PARA COUMARIC ACID; PINOCEMBRINE; SINAPIC ACID; UNCLASSIFIED DRUG; COMPLEMENTARY DNA; FLAVANONE DERIVATIVE; RECOMBINANT PROTEIN; TRANSCRIPTOME;

ALFALFA; ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL STRUCTURE; CIRCULAR DICHROISM; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; FERMENTATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLIC ENGINEERING; NONHUMAN; NUCLEOTIDE SEQUENCE; OC4CL1 GENE; OCCHI GENE; OCCHS2 GENE; OPTICAL ROTATION; ORNITHOGALUM; ORNITHOGALUM CAUDATUM; PH; PLANT GENE; PROTON NUCLEAR MAGNETIC RESONANCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE; ULTRAVIOLET SPECTROSCOPY; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MULTIGENE FAMILY; PROCEDURES;

BIOSYNTHETIC PATHWAYS; CARBON-13 MAGNETIC RESONANCE SPECTROSCOPY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; DNA, COMPLEMENTARY; FERMENTATION; FLAVANONES; GENE EXPRESSION REGULATION, PLANT; METABOLIC ENGINEERING; MULTIGENE FAMILY; ORNITHOGALUM; PROTON MAGNETIC RESONANCE SPECTROSCOPY; RECOMBINANT PROTEINS; TRANSCRIPTOME;

EID: 84958985421     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0424-8     Document Type: Article

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