Rapid addition of unlabeled silent solubility tags to proteins using a new substrate-fused sortase reagent

Journal of Biomolecular NMR

Volumn 64, Issue 3, 2016, Pages 197-205

  Amer, Brendan R ^a,b   MacDonald, Ramsay ^a,b   Jacobitz, Alex W ^a,b   Liauw, Brandon ^a,b   Clubb, Robert T ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Protein ligation; Silent solubility tag; Sortase; SUMO

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NUCLEOPHILE; PHOP PROTEIN; POLYHISTIDINE TAG; POLYPEPTIDE; PROTEIN; PROTEIN TAG; REAGENT; SMALL UBIQUITIN LIKE MODIFIER PROTEIN; SORTASE; SORTASE A; UNCLASSIFIED DRUG; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; SUMO 1 PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CENTRIFUGATION; CONJUGATION; HETERONUCLEAR SINGLE QUANTUM COHERENCE; NITROGEN NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTON NUCLEAR MAGNETIC RESONANCE; ROOM TEMPERATURE; SOLUBILITY; CHEMISTRY; NUCLEAR MAGNETIC RESONANCE;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CYSTEINE ENDOPEPTIDASES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEINS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUMO-1 PROTEIN;

EID: 84957558018     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-016-0019-z     Document Type: Article

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