Diabetes induced changes in podocyte morphology and gene expression evaluated using GFP transgenic podocytes

International Journal of Biological Sciences

Volumn 12, Issue 2, 2016, Pages 210-218

  Xu, Jianxiang ^a   Zheng, Shirong ^a   Kralik, Patricia M ^a   Krishnan, Laxminarayanan ^b   Huang, Hui ^a,d   Hoying, James B ^b   Cai, Lu ^a   Carlson, Edward C ^c   Tan, Yi ^a   Epstein, Paul N ^a  

^a University of Louisville School of Medicine   (United States)

^b UNIVERSITY OF LOUISVILLE   (United States)

^c UNIVERSITY OF NORTH DAKOTA   (United States)

^d Children s Hospital of Jiangxi Province   (China)

Author keywords

Chemokines; Diabetic nephropathy; Gene expression; Podocytes; Transgenic

Indexed keywords

GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; PODOCIN; SIGNAL PEPTIDE;

ANIMAL; DIABETIC NEPHROPATHY; EXPERIMENTAL DIABETES MELLITUS; GENE EXPRESSION; GENETICS; METABOLISM; MOUSE; PATHOLOGY; PODOCYTE; TRANSGENIC MOUSE; ULTRASTRUCTURE;

ANIMALS; DIABETES MELLITUS, EXPERIMENTAL; DIABETIC NEPHROPATHIES; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MEMBRANE PROTEINS; MICE; MICE, TRANSGENIC; PODOCYTES;

EID: 84956887961     PISSN: 14492288     EISSN: None     Source Type: Journal    
DOI: 10.7150/ijbs.13057     Document Type: Article

References (36)

1. Reidy K, Kang HM, Hostetter T, Susztak K. Molecular mechanisms of diabetic kidney disease. J Clin Invest. 2014; 124: 2333-40.
2. Zheng S, Carlson EC, Yang L, Kralik PM, Huang Y, Epstein PN. Podocyte-specific overexpression of the antioxidant metallothionein reduces diabetic nephropathy. J Am Soc Nephrol. 2008; 19: 2077-85.
3. Toyoda M, Najafian B, Kim Y, Caramori ML, Mauer M. Podocyte Detachment and Reduced Glomerular Capillary Endothelial Fenestration in Human Type 1 Diabetic Nephropathy. Diabetes. 2007.
4. Kriz W, Shirato I, Nagata M, LeHir M, Lemley KV. The podocyte's response to stress: the enigma of foot process effacement. Am J Physiol Renal Physiol. 2013; 304: F333-47.
5. Nakamura T, Ushiyama C, Suzuki S, Hara M, Shimada N, Ebihara I, et al. Urinary excretion of podocytes in patients with diabetic nephropathy. NephrolDialTransplant. 2000; 15: 1379-83.
6. Pagtalunan ME, Miller PL, Jumping-Eagle S, Nelson RG, Myers BD, Rennke HG, et al. Podocyte loss and progressive glomerular injury in type II diabetes. J Clin Invest. 1997; 99: 342-8.
7. Brunskill EW, Georgas K, Rumballe B, Little MH, Potter SS. Defining the molecular character of the developing and adult kidney podocyte. PLoS One. 2011; 6: e24640.
8. Boerries M, Grahammer F, Eiselein S, Buck M, Meyer C, Goedel M, et al. Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks. Kidney Int. 2013; 83: 1052-64.
9. Grgic I, Brooks CR, Hofmeister AF, Bijol V, Bonventre JV, Humphreys BD. Imaging of podocyte foot processes by fluorescence microscopy. Journal of the American Society of Nephrology : JASN. 2012; 23: 785-91.
10. Hohne M, Ising C, Hagmann H, Volker LA, Brahler S, Schermer B, et al. Light microscopic visualization of podocyte ultrastructure demonstrates oscillating glomerular contractions. Am J Pathol. 2013; 182: 332-8.
11. Moeller MJ, Sanden SK, Soofi A, Wiggins RC, Holzman LB. Two gene fragments that direct podocyte-specific expression in transgenic mice. Journal of the American Society of Nephrology : JASN. 2002; 13: 1561-7.
12. Cormack BP, Valdivia RH, Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996; 173: 33-8.
13. Yokoyama T, Silversides DW, Waymire KG, Kwon BS, Takeuchi T, Overbeek PA. Conserved cysteine to serine mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice. Nucleic acids research. 1990; 18: 7293-8.
14. Ribar TJ, Epstein PN, Overbeek PA, Means AR. Targeted overexpression of an inactive calmodulin that binds Ca2+ to the mouse pancreatic beta-cell results in impaired secretion and chronic hyperglycemia. Endocrinology. 1995; 136: 106-15.
15. Zheng S, Noonan WT, Metreveli NS, Coventry S, Kralik PM, Carlson EC, et al. Development of Late-Stage Diabetic Nephropathy in OVE26 Diabetic Mice. Diabetes. 2004; 53: 3248-57.
16. Chua S, Jr., Liu SM, Li Q, Yang L, Thassanapaff VT, Fisher P. Differential beta cell responses to hyperglycaemia and insulin resistance in two novel congenic strains of diabetes (FVB-Lepr (db)) and obese (DBA- Lep (ob)) mice. Diabetologia. 2002; 45: 976-90.
17. Qi Z, Fujita H, Jin J, Davis LS, Wang Y, Fogo AB, et al. Characterization of susceptibility of inbred mouse strains to diabetic nephropathy. Diabetes. 2005; 54: 2628-37.
18. Epstein PN, Overbeek PA, Means AR. Calmodulin-induced early-onset diabetes in transgenic mice. Cell. 1989; 58: 1067-73.
19. Takemoto M, Asker N, Gerhardt H, Lundkvist A, Johansson BR, Saito Y, et al. A new method for large scale isolation of kidney glomeruli from mice. Am- JPathol. 2002; 161: 799-805.
20. Wang J, Wang Q, Watson LJ, Jones SP, Epstein PN. Cardiac overexpression of 8-oxoguanine DNA glycosylase 1 protects mitochondrial DNA and reduces cardiac fibrosis following transaortic constriction. American journal of physiology Heart and circulatory physiology. 2011; 301: H2073-80.
21. Yang L, Zheng S, Epstein PN. Metallothionein over-expression in podocytes reduces adriamycin nephrotoxicity. Free Radic Res. 2009; 43: 174-82.
22. Krishnan L, Underwood CJ, Maas S, Ellis BJ, Kode TC, Hoying JB, et al. Effect of mechanical boundary conditions on orientation of angiogenic microvessels. Cardiovascular Research. 2008; 78: 324-32.
23. Doublier S, Salvidio G, Lupia E, Ruotsalainen V, Verzola D, Deferrari G, et al. Nephrin expression is reduced in human diabetic nephropathy: evidence for a distinct role for glycated albumin and angiotensin II. Diabetes. 2003; 52: 1023-30.
24. Woroniecka KI, Park AS, Mohtat D, Thomas DB, Pullman JM, Susztak K. Transcriptome analysis of human diabetic kidney disease. Diabetes. 2011; 60: 2354-69.
25. Lorenzen J, Shah R, Biser A, Staicu SA, Niranjan T, Garcia AM, et al. The role of osteopontin in the development of albuminuria. J Am Soc Nephrol. 2008; 19: 884-90.
26. Susztak K, Bottinger E, Novetsky A, Liang D, Zhu Y, Ciccone E, et al. Molecular profiling of diabetic mouse kidney reveals novel genes linked to glomerular disease. Diabetes. 2004; 53: 784-94.
27. Chung AC, Lan HY. Chemokines in renal injury. J Am Soc Nephrol. 2011; 22: 802-9.
28. Ellis EN, Steffes MW, Chavers B, Mauer SM. Observations of glomerular epithelial cell structure in patients with type I diabetes mellitus. Kidney Int. 1987; 32: 736-41.
29. Tao J, Polumbo C, Reidy K, Sweetwyne M, Susztak K. A multicolor podocyte reporter highlights heterogeneous podocyte changes in focal segmental glomerulosclerosis. Kidney Int. 2014; 85: 972-80.
30. Carlson EC, Chhoun JM, Laturnus DI, Bikash KC, Berg B, Zheng S, et al. Podocyte-specific overexpression of metallothionein mitigates diabetic complications in the glomerular filtration barrier and glomerular histoarchitecture: a transmission electron microscopy stereometric analysis. Diabetes Metab Res Rev. 2013; 29: 113-24.
31. Hodgin JB, Borczuk AC, Nasr SH, Markowitz GS, Nair V, Martini S, et al. A molecular profile of focal segmental glomerulosclerosis from formalin-fixed, paraffin-embedded tissue. The American journal of pathology. 2010; 177: 1674-86.
32. Xu J, Huang Y, Li F, Zheng S, Epstein PN. FVB mouse genotype confers susceptibility to OVE26 diabetic albuminuria. Am J Physiol Renal Physiol. 2010; 299: F487-94.
33. Burger D, Thibodeau JF, Holterman CE, Burns KD, Touyz RM, Kennedy CR. Urinary podocyte microparticles identify prealbuminuric diabetic glomerular injury. J Am Soc Nephrol. 2014; 25: 1401-7.
34. Nicholas SB, Liu J, Kim J, Ren Y, Collins AR, Nguyen L, et al. Critical role for osteopontin in diabetic nephropathy. Kidney Int. 2010; 77: 588-600.
35. Anderberg RJ, Meek RL, Hudkins KL, Cooney SK, Alpers CE, Leboeuf RC, et al. Serum amyloid A and inflammation in diabetic kidney disease and podocytes. Lab Invest. 2015; 95: 250-62.
36. Grgic I, Hofmeister AF, Genovese G, Bernhardy AJ, Sun H, Maarouf OH, et al. Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo. Kidney international. 2014; 86: 1116-29.