Fully automatized high-throughput enzyme library screening using a robotic platform

Biotechnology and Bioengineering

Volumn 113, Issue 7, 2016, Pages 1421-1432

  Dörr, Mark ^a   Fibinger, Michael P C ^a   Last, Daniel ^a   Schmidt, Sandy ^a   Santos Aberturas, Javier ^a   Böttcher, Dominique ^a   Hummel, Anke ^a   Vickers, Clare ^a   Voss, Moritz ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Activity assay; Enzymes; Growth conditions; High throughput screening; Protein engineering; Robotic platform

Indexed keywords

BIOCHEMICAL ENGINEERING; GENETIC ENGINEERING; LIBRARIES; PROTEINS; ROBOTICS; THROUGHPUT;

ACTIVITY ASSAYS; GROWTH CONDITIONS; HIGH THROUGHPUT SCREENING; PROTEIN ENGINEERING; ROBOTIC PLATFORMS;

ENZYMES;

AMIDASE; AMINE; AMINOACYLASE; AMINOTRANSFERASE; BACTERIAL ENZYME; DEHALOGENASE; ENZYME; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; MOLECULAR LIBRARY;

ARTICLE; BACTERIAL GROWTH; BAEYER VILLIGER REACTION; BARCODE READER; BIOCATALYSIS; CENTRIFUGE; COMPUTER PROGRAM; CYTOLYSIS; DEVICES; ELECTRONICS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME STABILITY; ENZYME SYNTHESIS; EQUIPMENT DESIGN; ESCHERICHIA COLI; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; INCUBATOR; MICROTITER PLATE; MOLECULAR LIBRARY; NEGATIVE FEEDBACK; PIN HEAD MICROBIAL COLONY PICKER; PROCESS OPTIMIZATION; PROTEIN STABILITY; ROBOTICS; SPECTROPHOTOMETER; TURN TABLE; ENZYME ASSAY; LABORATORY AUTOMATION; PROCEDURES; PROTEIN ENGINEERING;

AUTOMATION, LABORATORY; ENZYME ASSAYS; EQUIPMENT DESIGN; HIGH-THROUGHPUT SCREENING ASSAYS; PROTEIN ENGINEERING; ROBOTICS; SMALL MOLECULE LIBRARIES; TRANSAMINASES;

EID: 84956866816     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25925     Document Type: Article

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