Target-selective joint polymerase chain reaction: A robust and rapid method for high-throughput production of recombinant monoclonal antibodies from single cells

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Yoshioka, Megumi ^a   Kurosawa, Nobuyuki ^a   Isobe, Masaharu ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY EXPRESSION; CDNA ENDS; CDNA SYNTHESIS; CELL-BASED; ESSENTIAL ELEMENTS; EXPRESSION CONSTRUCTS; GENE CLONING; GENE FRAGMENTS; HIGH-THROUGHPUT; IMMUNOGLOBULIN GENES; MAGNETIC BEADS; OVERLAP EXTENSION POLYMERASE CHAIN REACTIONS; OVERLAPPING AREA; PLASMA CELLS; RAPID METHOD; RECOMBINANT MONOCLONAL ANTIBODY; SHORT PERIODS; SINGLE CELLS; THERAPEUTIC ANTIBODIES;

CLONE CELLS; CLONING; FUNCTIONAL POLYMERS; GENE EXPRESSION; MONOCLONAL ANTIBODIES; POLYMERIZATION; SURFACE PLASMON RESONANCE; SYNTHESIS (CHEMICAL); THROUGHPUT;

POLYMERASE CHAIN REACTION;

COMPLEMENTARY DNA; DNA FRAGMENT; IMMUNOGLOBULIN; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; PRIMER DNA; RECOMBINANT PROTEIN;

ARTICLE; DNA SYNTHESIS; GENE AMPLIFICATION; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; HIGH THROUGHPUT SEQUENCING; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SINGLE CELL ANALYSIS; TARGET SELECTIVE JOINT POLYMERASE CHAIN REACTION; AGAR GEL ELECTROPHORESIS; ANIMAL; BIOSYNTHESIS; CHEMISTRY; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; METHODOLOGY; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PROTEIN ENGINEERING; ROBOTICS; TIME;

ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; HIGH-THROUGHPUT SCREENING ASSAYS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; ROBOTICS; TIME FACTORS;

EID: 79960560683     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-75     Document Type: Article

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