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Volumn 34, Issue 24, 2015, Pages 3059-3073

Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex

Author keywords

B complex; electron microscopy; immunolabelling; protein localisation; spliceosome

Indexed keywords

BRR2 PROTEIN, S CEREVISIAE; PROTEIN BINDING; RNA HELICASE; SACCHAROMYCES CEREVISIAE PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN;

EID: 84955170008     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.15252/embj.201592022     Document Type: Article
Times cited : (10)

References (60)
  • 1
    • 0033569743 scopus 로고    scopus 로고
    • A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro
    • Achsel T, Brahms H, Kastner B, Bachi A, Wilm M, Lührmann R, (1999) A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. EMBO J 18: 5789-5802
    • (1999) EMBO J , vol.18 , pp. 5789-5802
    • Achsel, T.1    Brahms, H.2    Kastner, B.3    Bachi, A.4    Wilm, M.5    Lührmann, R.6
  • 3
    • 31044438832 scopus 로고    scopus 로고
    • Prp8p dissection reveals domain structure and protein interaction sites
    • Boon K-L, Norman CM, Grainger RJ, Newman AJ, Beggs JD, (2006) Prp8p dissection reveals domain structure and protein interaction sites. RNA 12: 198-205
    • (2006) RNA , vol.12 , pp. 198-205
    • Boon, K.-L.1    Norman, C.M.2    Grainger, R.J.3    Newman, A.J.4    Beggs, J.D.5
  • 4
    • 0033636518 scopus 로고    scopus 로고
    • Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
    • Das R, Zhou Z, Reed R, (2000) Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell 5: 779-787
    • (2000) Mol Cell , vol.5 , pp. 779-787
    • Das, R.1    Zhou, Z.2    Reed, R.3
  • 5
    • 0027418576 scopus 로고
    • The portal protein of bacteriophage SPP1: A DNA pump with 13-fold symmetry
    • Dube P, Tavares P, Lurz R, van Heel M, (1993) The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry. EMBO J 12: 1303-1309
    • (1993) EMBO J , vol.12 , pp. 1303-1309
    • Dube, P.1    Tavares, P.2    Lurz, R.3    Van Heel, M.4
  • 7
    • 0030976931 scopus 로고    scopus 로고
    • An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2
    • Fabrizio P, Laggerbauer B, Lauber J, Lane WS, Lührmann R, (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16: 4092-4106
    • (1997) EMBO J , vol.16 , pp. 4092-4106
    • Fabrizio, P.1    Laggerbauer, B.2    Lauber, J.3    Lane, W.S.4    Lührmann, R.5
  • 8
    • 70449625597 scopus 로고    scopus 로고
    • The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome
    • Fabrizio P, Dannenberg J, Dube P, Kastner B, Stark H, Urlaub H, Lührmann R, (2009) The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. Mol Cell 36: 593-608
    • (2009) Mol Cell , vol.36 , pp. 593-608
    • Fabrizio, P.1    Dannenberg, J.2    Dube, P.3    Kastner, B.4    Stark, H.5    Urlaub, H.6    Lührmann, R.7
  • 9
    • 84893110707 scopus 로고    scopus 로고
    • Structural studies of the spliceosome: Zooming into the heart of the machine
    • Galej WP, Nguyen THD, Newman AJ, Nagai K, (2014) Structural studies of the spliceosome: zooming into the heart of the machine. Curr Opin Struct Biol 25: 57-66
    • (2014) Curr Opin Struct Biol , vol.25 , pp. 57-66
    • Galej, W.P.1    Nguyen, T.H.D.2    Newman, A.J.3    Nagai, K.4
  • 10
    • 0038670209 scopus 로고    scopus 로고
    • Molecular architecture of the multiprotein splicing factor SF3b
    • Golas MM, Sander B, Will CL, Lührmann R, Stark H, (2003) Molecular architecture of the multiprotein splicing factor SF3b. Science 300: 980-984
    • (2003) Science , vol.300 , pp. 980-984
    • Golas, M.M.1    Sander, B.2    Will, C.L.3    Lührmann, R.4    Stark, H.5
  • 11
    • 0033575711 scopus 로고    scopus 로고
    • Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP
    • Gottschalk A, Neubauer G, Banroques J, Mann M, Lührmann R, Fabrizio P, (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J 18: 4535-4548
    • (1999) EMBO J , vol.18 , pp. 4535-4548
    • Gottschalk, A.1    Neubauer, G.2    Banroques, J.3    Mann, M.4    Lührmann, R.5    Fabrizio, P.6
  • 12
    • 0035052524 scopus 로고    scopus 로고
    • A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly
    • Gottschalk A, Bartels C, Neubauer G, Lührmann R, Fabrizio P, (2001) A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol 21: 3037-3046
    • (2001) Mol Cell Biol , vol.21 , pp. 3037-3046
    • Gottschalk, A.1    Bartels, C.2    Neubauer, G.3    Lührmann, R.4    Fabrizio, P.5
  • 13
    • 17844395704 scopus 로고    scopus 로고
    • Prp8 protein: At the heart of the spliceosome
    • Grainger RJ, Beggs JD, (2005) Prp8 protein: at the heart of the spliceosome. RNA 11: 533-557
    • (2005) RNA , vol.11 , pp. 533-557
    • Grainger, R.J.1    Beggs, J.D.2
  • 15
    • 84868592766 scopus 로고    scopus 로고
    • Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning
    • Hahn D, Kudla G, Tollervey D, Beggs JD, (2012) Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning. Genes Dev 26: 2408-2421
    • (2012) Genes Dev , vol.26 , pp. 2408-2421
    • Hahn, D.1    Kudla, G.2    Tollervey, D.3    Beggs, J.D.4
  • 16
    • 0019728717 scopus 로고
    • Use of multivariate statistics in analysing the images of biological macromolecules
    • Van Heel M, Frank J, (1981) Use of multivariate statistics in analysing the images of biological macromolecules. Ultramicroscopy 6: 187-194
    • (1981) Ultramicroscopy , vol.6 , pp. 187-194
    • Van Heel, M.1    Frank, J.2
  • 17
    • 0021645447 scopus 로고
    • Multivariate statistical classification of noisy images (randomly oriented biological macromolecules)
    • Van Heel M, (1984) Multivariate statistical classification of noisy images (randomly oriented biological macromolecules). Ultramicroscopy 13: 165-183
    • (1984) Ultramicroscopy , vol.13 , pp. 165-183
    • Van Heel, M.1
  • 19
    • 0027923620 scopus 로고
    • Involvement of U6 snRNA in 5' splice site selection
    • Kandels-Lewis S, Séraphin B, (1993) Involvement of U6 snRNA in 5' splice site selection. Science 262: 2035-2039
    • (1993) Science , vol.262 , pp. 2035-2039
    • Kandels-Lewis, S.1    Séraphin, B.2
  • 20
    • 0002821311 scopus 로고    scopus 로고
    • Purification and electron microscopy of spliceosomal snRNPs
    • Schenkel D.J. (ed.), Berlin Heidelberg: Springer
    • Kastner B, (1998) Purification and electron microscopy of spliceosomal snRNPs. In RNP Particles, Splicing and Autoimmune Diseases, Schenkel DJ, (ed.), pp 95-140. Berlin Heidelberg: Springer
    • (1998) RNP Particles, Splicing and Autoimmune Diseases , pp. 95-140
    • Kastner, B.1
  • 23
    • 0032515969 scopus 로고    scopus 로고
    • The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro
    • Laggerbauer B, Achsel T, Lührmann R, (1998) The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro. Proc Natl Acad Sci USA 95: 4188-4192
    • (1998) Proc Natl Acad Sci USA , vol.95 , pp. 4188-4192
    • Laggerbauer, B.1    Achsel, T.2    Lührmann, R.3
  • 24
    • 0027739852 scopus 로고
    • Mutations in U6 snRNA that alter splice site specificity: Implications for the active site
    • Lesser CF, Guthrie C, (1993) Mutations in U6 snRNA that alter splice site specificity: implications for the active site. Science 262: 1982-1988
    • (1993) Science , vol.262 , pp. 1982-1988
    • Lesser, C.F.1    Guthrie, C.2
  • 25
    • 84858800333 scopus 로고    scopus 로고
    • Structure and assembly of the SF3a splicing factor complex of U2 snRNP
    • Lin P-C, Xu R-M, (2012) Structure and assembly of the SF3a splicing factor complex of U2 snRNP. EMBO J 31: 1579-1590
    • (2012) EMBO J , vol.31 , pp. 1579-1590
    • Lin, P.-C.1    Xu, R.-M.2
  • 27
    • 60349110387 scopus 로고    scopus 로고
    • Structural mapping of spliceosomes by electron microscopy
    • Lührmann R, Stark H, (2009) Structural mapping of spliceosomes by electron microscopy. Curr Opin Struct Biol 19: 96-102
    • (2009) Curr Opin Struct Biol , vol.19 , pp. 96-102
    • Lührmann, R.1    Stark, H.2
  • 28
    • 0026486883 scopus 로고
    • A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome
    • Madhani HD, Guthrie C, (1992) A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71: 803-817
    • (1992) Cell , vol.71 , pp. 803-817
    • Madhani, H.D.1    Guthrie, C.2
  • 29
    • 0037980050 scopus 로고    scopus 로고
    • Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast
    • McPheeters DS, Muhlenkamp P, (2003) Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast. Mol Cell Biol 23: 4174-4186
    • (2003) Mol Cell Biol , vol.23 , pp. 4174-4186
    • McPheeters, D.S.1    Muhlenkamp, P.2
  • 30
    • 0036297657 scopus 로고    scopus 로고
    • Direct probing of RNA structure and RNA-protein interactions in purified HeLa cell's and yeast spliceosomal U4/U6.U5 tri-snRNP particles
    • Mougin A, Gottschalk A, Fabrizio P, Lührmann R, Branlant C, (2002) Direct probing of RNA structure and RNA-protein interactions in purified HeLa cell's and yeast spliceosomal U4/U6.U5 tri-snRNP particles. J Mol Biol 317: 631-649
    • (2002) J Mol Biol , vol.317 , pp. 631-649
    • Mougin, A.1    Gottschalk, A.2    Fabrizio, P.3    Lührmann, R.4    Branlant, C.5
  • 31
    • 84868583242 scopus 로고    scopus 로고
    • The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA
    • Mozaffari-Jovin S, Santos KF, Hsiao H-H, Will CL, Urlaub H, Wahl MC, Lührmann R, (2012) The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA. Genes Dev 26: 2422-2434
    • (2012) Genes Dev , vol.26 , pp. 2422-2434
    • Mozaffari-Jovin, S.1    Santos, K.F.2    Hsiao, H.-H.3    Will, C.L.4    Urlaub, H.5    Wahl, M.C.6    Lührmann, R.7
  • 33
    • 84878849387 scopus 로고    scopus 로고
    • Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site
    • Nguyen THD, Li J, Galej WP, Oshikane H, Newman AJ, Nagai K, (2013) Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site. Structure 21: 910-919
    • (2013) Structure , vol.21 , pp. 910-919
    • Nguyen, T.H.D.1    Li, J.2    Galej, W.P.3    Oshikane, H.4    Newman, A.J.5    Nagai, K.6
  • 35
    • 84869152716 scopus 로고    scopus 로고
    • Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!
    • Nielsen KH, Staley JP, (2012) Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!. Genes Dev 26: 2461-2467
    • (2012) Genes Dev , vol.26 , pp. 2461-2467
    • Nielsen, K.H.1    Staley, J.P.2
  • 36
    • 0037107418 scopus 로고    scopus 로고
    • Hierarchical, clustered protein interactions with U4/U6 snRNA: A biochemical role for U4/U6 proteins
    • Nottrott S, Urlaub H, Lührmann R, (2002) Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins. EMBO J 21: 5527-5538
    • (2002) EMBO J , vol.21 , pp. 5527-5538
    • Nottrott, S.1    Urlaub, H.2    Lührmann, R.3
  • 38
    • 0032537739 scopus 로고    scopus 로고
    • RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
    • Raghunathan PL, Guthrie C, (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr Biol 8: 847-855
    • (1998) Curr Biol , vol.8 , pp. 847-855
    • Raghunathan, P.L.1    Guthrie, C.2
  • 39
    • 0032828715 scopus 로고    scopus 로고
    • A generic protein purification method for protein complex characterization and proteome exploration
    • Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Séraphin B, (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol 17: 1030-1032
    • (1999) Nat Biotechnol , vol.17 , pp. 1030-1032
    • Rigaut, G.1    Shevchenko, A.2    Rutz, B.3    Wilm, M.4    Mann, M.5    Séraphin, B.6
  • 41
    • 33749672830 scopus 로고    scopus 로고
    • Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy
    • Sander B, Golas MM, Makarov EM, Brahms H, Kastner B, Lührmann R, Stark H, (2006) Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy. Mol Cell 24: 267-278
    • (2006) Mol Cell , vol.24 , pp. 267-278
    • Sander, B.1    Golas, M.M.2    Makarov, E.M.3    Brahms, H.4    Kastner, B.5    Lührmann, R.6    Stark, H.7
  • 42
    • 33748778456 scopus 로고    scopus 로고
    • RNA structural requirements for the association of the spliceosomal hPrp31 protein with the U4 and U4atac small nuclear ribonucleoproteins
    • Schultz A, Nottrott S, Hartmuth K, Lührmann R, (2006) RNA structural requirements for the association of the spliceosomal hPrp31 protein with the U4 and U4atac small nuclear ribonucleoproteins. J Biol Chem 281: 28278-28286
    • (2006) J Biol Chem , vol.281 , pp. 28278-28286
    • Schultz, A.1    Nottrott, S.2    Hartmuth, K.3    Lührmann, R.4
  • 43
    • 0001500510 scopus 로고
    • Three-dimensional structure of an intact human immunoglobulin
    • Silverton EW, Navia MA, Davies DR, (1977) Three-dimensional structure of an intact human immunoglobulin. Proc Natl Acad Sci USA 74: 5140-5144
    • (1977) Proc Natl Acad Sci USA , vol.74 , pp. 5140-5144
    • Silverton, E.W.1    Navia, M.A.2    Davies, D.R.3
  • 44
    • 33746441457 scopus 로고    scopus 로고
    • The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase
    • Small EC, Leggett SR, Winans AA, Staley JP, (2006) The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase. Mol Cell 23: 389-399
    • (2006) Mol Cell , vol.23 , pp. 389-399
    • Small, E.C.1    Leggett, S.R.2    Winans, A.A.3    Staley, J.P.4
  • 45
    • 0028209981 scopus 로고
    • Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly
    • Staknis D, Reed R, (1994) Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly. Mol Cell Biol 14: 2994-3005
    • (1994) Mol Cell Biol , vol.14 , pp. 2994-3005
    • Staknis, D.1    Reed, R.2
  • 46
    • 0033010430 scopus 로고    scopus 로고
    • An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p
    • Staley JP, Guthrie C, (1999) An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p. Mol Cell 3: 55-64
    • (1999) Mol Cell , vol.3 , pp. 55-64
    • Staley, J.P.1    Guthrie, C.2
  • 47
    • 0035945611 scopus 로고    scopus 로고
    • Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle
    • Stark H, Dube P, Lührmann R, Kastner B, (2001) Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle. Nature 409: 539-542
    • (2001) Nature , vol.409 , pp. 539-542
    • Stark, H.1    Dube, P.2    Lührmann, R.3    Kastner, B.4
  • 48
    • 33745050151 scopus 로고    scopus 로고
    • Cryo-electron microscopy of spliceosomal components
    • Stark H, Lührmann R, (2006) Cryo-electron microscopy of spliceosomal components. Annu Rev Biophys Biomol Struct 35: 435-457
    • (2006) Annu Rev Biophys Biomol Struct , vol.35 , pp. 435-457
    • Stark, H.1    Lührmann, R.2
  • 49
    • 0033595016 scopus 로고    scopus 로고
    • Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins
    • Stevens SW, Abelson J, (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci USA 96: 7226-7231
    • (1999) Proc Natl Acad Sci USA , vol.96 , pp. 7226-7231
    • Stevens, S.W.1    Abelson, J.2
  • 50
    • 0035161567 scopus 로고    scopus 로고
    • Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae
    • Stevens SW, Barta I, Ge HY, Moore RE, Young MK, Lee TD, Abelson J, (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7: 1543-1553
    • (2001) RNA , vol.7 , pp. 1543-1553
    • Stevens, S.W.1    Barta, I.2    Ge, H.Y.3    Moore, R.E.4    Young, M.K.5    Lee, T.D.6    Abelson, J.7
  • 51
    • 60349104299 scopus 로고    scopus 로고
    • The spliceosome: Design principles of a dynamic RNP machine
    • Wahl MC, Will CL, Lührmann R, (2009) The spliceosome: design principles of a dynamic RNP machine. Cell 136: 701-718
    • (2009) Cell , vol.136 , pp. 701-718
    • Wahl, M.C.1    Will, C.L.2    Lührmann, R.3
  • 52
    • 28544447481 scopus 로고    scopus 로고
    • Interactions of the yeast SF3b splicing factor
    • Wang Q, He J, Lynn B, Rymond BC, (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25: 10745-10754
    • (2005) Mol Cell Biol , vol.25 , pp. 10745-10754
    • Wang, Q.1    He, J.2    Lynn, B.3    Rymond, B.C.4
  • 54
    • 68249149579 scopus 로고    scopus 로고
    • Exon, intron and splice site locations in the spliceosomal B complex
    • Wolf E, Kastner B, Deckert J, Merz C, Stark H, Lührmann R, (2009) Exon, intron and splice site locations in the spliceosomal B complex. EMBO J 28: 2283-2292
    • (2009) EMBO J , vol.28 , pp. 2283-2292
    • Wolf, E.1    Kastner, B.2    Deckert, J.3    Merz, C.4    Stark, H.5    Lührmann, R.6
  • 56
    • 0032525135 scopus 로고    scopus 로고
    • Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation
    • Xie J, Beickman K, Otte E, Rymond BC, (1998) Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation. EMBO J 17: 2938-2946
    • (1998) EMBO J , vol.17 , pp. 2938-2946
    • Xie, J.1    Beickman, K.2    Otte, E.3    Rymond, B.C.4
  • 57
    • 0025177036 scopus 로고
    • The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA
    • Xu Y, Petersen-Bjørn S, Friesen JD, (1990) The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA. Mol Cell Biol 10: 1217-1225
    • (1990) Mol Cell Biol , vol.10 , pp. 1217-1225
    • Xu, Y.1    Petersen-Bjørn, S.2    Friesen, J.D.3
  • 58
    • 84942474131 scopus 로고    scopus 로고
    • Structure of a yeast spliceosome at 3.6-angstrom resolution
    • Yan C, Hang J, Wan R, Huang M, Wong CCL, Shi Y, (2015) Structure of a yeast spliceosome at 3.6-angstrom resolution. Science 349: 1182-1191
    • (2015) Science , vol.349 , pp. 1182-1191
    • Yan, C.1    Hang, J.2    Wan, R.3    Huang, M.4    Wong, C.C.L.5    Shi, Y.6
  • 59
    • 40649103446 scopus 로고    scopus 로고
    • Purification of functional RNA-protein complexes using MS2-MBP
    • Chapter 27: Unit 27.3
    • Zhou Z, Reed R, (2003) Purification of functional RNA-protein complexes using MS2-MBP. Curr Protoc Mol Biol Chapter 27: Unit 27.3
    • (2003) Curr Protoc Mol Biol
    • Zhou, Z.1    Reed, R.2
  • 60
    • 84893774907 scopus 로고    scopus 로고
    • Crystal structures of the Lsm complex bound to the 3' end sequence of U6 small nuclear RNA
    • Zhou L, Hang J, Zhou Y, Wan R, Lu G, Yin P, Yan C, Shi Y, (2014) Crystal structures of the Lsm complex bound to the 3' end sequence of U6 small nuclear RNA. Nature 506: 116-120
    • (2014) Nature , vol.506 , pp. 116-120
    • Zhou, L.1    Hang, J.2    Zhou, Y.3    Wan, R.4    Lu, G.5    Yin, P.6    Yan, C.7    Shi, Y.8


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