Binding of the human Prp31 Nop domain to a composite RNA-protein platform in U4 snRNP

Science

Volumn 316, Issue 5821, 2007, Pages 115-120

  Liu, Sunbin ^a   Li, Ping ^a   Dybkov, Olexandr ^a   Nottrott, Stephanie ^a   Hartmuth, Klaus ^a   Lührmann, Reinhard ^a   Carlomagno, Teresa ^a   Wahl, Markus C ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN DERIVATIVE; PROTEIN PRP31; SMALL NUCLEAR RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG;

BIOASSAY; CHEMICAL BINDING; PROTEIN; RNA;

ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; HUMAN; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; RETINITIS PIGMENTOSA; SPLICEOSOME; TWO HYBRID SYSTEM;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CARRIER PROTEINS; EYE PROTEINS; HUMANS; HYDROPHOBICITY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RETINITIS PIGMENTOSA; RIBONUCLEOPROTEIN, U4-U6 SMALL NUCLEAR; RNA, SMALL NUCLEAR;

EID: 34147095881     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1137924     Document Type: Article

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35. We thank S. Bhattacharya (University College London, UK) for supplying pTriEx-hPrp31, H. Urlaub for mass spectrometric analyses, G. Bourenkov (European Molecular Biology Laboratory, Hamburg, Germany) and the group of H. Bartunik (beamline BW6, Deutsches Elektronen Synchrotron, Hamburg, Germany) for beamline support, and C. Will for fruitful discussions and critical reading of the manuscript. This work was supported by the Max-Planck-Gesellschaft (to R.L., T.C., and M.C.W.), the Deutsche Forschungsgemeinschaft (LU294/12-3), the Fonds der Chemischen Industrie (to R.L.), and the Ernst-Jung-Stiftung (to R.L.). Coordinates and structure factors have been deposited with the RCSB Protein Data Bank (www.rcsb.org/pdb/) under accession code 2OZB and will be released upon publication. The authors declare that they have no competing financial interests.