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Volumn 24, Issue 2, 2006, Pages 267-278

Organization of Core Spliceosomal Components U5 snRNA Loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as Revealed by Electron Cryomicroscopy

Author keywords

RNA

Indexed keywords

NUCLEOTIDE; PROTEIN SUBUNIT; SMALL NUCLEAR RNA;

EID: 33749672830     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcel.2006.08.021     Document Type: Article
Times cited : (69)

References (41)
  • 1
    • 0031741144 scopus 로고    scopus 로고
    • The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein
    • Achsel T., Ahrens K., Brahms H., Teigelkamp S., and Lührmann R. The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Mol. Cell. Biol. 18 (1998) 6756-6766
    • (1998) Mol. Cell. Biol. , vol.18 , pp. 6756-6766
    • Achsel, T.1    Ahrens, K.2    Brahms, H.3    Teigelkamp, S.4    Lührmann, R.5
  • 2
    • 0033569743 scopus 로고    scopus 로고
    • A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro
    • Achsel T., Brahms H., Kastner B., Bachi A., Wilm M., and Lührmann R. A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. EMBO J. 18 (1999) 5789-5802
    • (1999) EMBO J. , vol.18 , pp. 5789-5802
    • Achsel, T.1    Brahms, H.2    Kastner, B.3    Bachi, A.4    Wilm, M.5    Lührmann, R.6
  • 4
    • 4444233084 scopus 로고    scopus 로고
    • Three-dimensional structure of the native spliceosome by cryo-electron microscopy
    • Azubel M., Wolf S.G., Sperling J., and Sperling R. Three-dimensional structure of the native spliceosome by cryo-electron microscopy. Mol. Cell 15 (2004) 833-839
    • (2004) Mol. Cell , vol.15 , pp. 833-839
    • Azubel, M.1    Wolf, S.G.2    Sperling, J.3    Sperling, R.4
  • 5
    • 0025951304 scopus 로고
    • Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells
    • Behrens S.E., and Lührmann R. Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells. Genes Dev. 5 (1991) 1439-1452
    • (1991) Genes Dev. , vol.5 , pp. 1439-1452
    • Behrens, S.E.1    Lührmann, R.2
  • 6
    • 0026661948 scopus 로고
    • Protein components specifically associated with prespliceosome and spliceosome complexes
    • Bennett M., Michaud S., Kingston J., and Reed R. Protein components specifically associated with prespliceosome and spliceosome complexes. Genes Dev. 6 (1992) 1986-2000
    • (1992) Genes Dev. , vol.6 , pp. 1986-2000
    • Bennett, M.1    Michaud, S.2    Kingston, J.3    Reed, R.4
  • 7
    • 0023656329 scopus 로고
    • A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs
    • Bochnig P., Reuter R., Bringmann P., and Lührmann R. A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs. Eur. J. Biochem. 168 (1987) 461-467
    • (1987) Eur. J. Biochem. , vol.168 , pp. 461-467
    • Bochnig, P.1    Reuter, R.2    Bringmann, P.3    Lührmann, R.4
  • 9
    • 0033636518 scopus 로고    scopus 로고
    • Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
    • Das R., Zhou Z., and Reed R. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol. Cell 5 (2000) 779-787
    • (2000) Mol. Cell , vol.5 , pp. 779-787
    • Das, R.1    Zhou, Z.2    Reed, R.3
  • 10
    • 0031719371 scopus 로고    scopus 로고
    • Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae
    • Dix I., Russell C.S., O'Keefe R.T., Newman A.J., and Beggs J.D. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4 (1998) 1675-1686
    • (1998) RNA , vol.4 , pp. 1675-1686
    • Dix, I.1    Russell, C.S.2    O'Keefe, R.T.3    Newman, A.J.4    Beggs, J.D.5
  • 11
    • 0027418576 scopus 로고
    • The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry
    • Dube P., Tavares P., Lurz R., and van Heel M. The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry. EMBO J. 12 (1993) 1303-1309
    • (1993) EMBO J. , vol.12 , pp. 1303-1309
    • Dube, P.1    Tavares, P.2    Lurz, R.3    van Heel, M.4
  • 12
    • 0038670209 scopus 로고    scopus 로고
    • Molecular architecture of the multiprotein splicing factor SF3b
    • Golas M.M., Sander B., Will C.L., Lührmann R., and Stark H. Molecular architecture of the multiprotein splicing factor SF3b. Science 300 (2003) 980-984
    • (2003) Science , vol.300 , pp. 980-984
    • Golas, M.M.1    Sander, B.2    Will, C.L.3    Lührmann, R.4    Stark, H.5
  • 13
    • 15244359121 scopus 로고    scopus 로고
    • Major conformational change in the complex SF3b upon integration into the spliceosomal U11/U12 di-snRNP as revealed by electron cryomicroscopy
    • Golas M.M., Sander B., Will C.L., Lührmann R., and Stark H. Major conformational change in the complex SF3b upon integration into the spliceosomal U11/U12 di-snRNP as revealed by electron cryomicroscopy. Mol. Cell 17 (2005) 869-883
    • (2005) Mol. Cell , vol.17 , pp. 869-883
    • Golas, M.M.1    Sander, B.2    Will, C.L.3    Lührmann, R.4    Stark, H.5
  • 14
    • 0000313739 scopus 로고
    • Similarity measures between images. Exact filters for general geometry 3D reconstruction
    • Harauz G., and van Heel M. Similarity measures between images. Exact filters for general geometry 3D reconstruction. Optik 73 (1986) 146-156
    • (1986) Optik , vol.73 , pp. 146-156
    • Harauz, G.1    van Heel, M.2
  • 16
    • 1442360371 scopus 로고    scopus 로고
    • Three-dimensional structure of C complex spliceosomes by electron microscopy
    • Jurica M.S., Sousa D., Moore M.J., and Grigorieff N. Three-dimensional structure of C complex spliceosomes by electron microscopy. Nat. Struct. Mol. Biol. 11 (2004) 265-269
    • (2004) Nat. Struct. Mol. Biol. , vol.11 , pp. 265-269
    • Jurica, M.S.1    Sousa, D.2    Moore, M.J.3    Grigorieff, N.4
  • 17
    • 0002821311 scopus 로고    scopus 로고
    • Purification and electron microscopy of spliceosomal snRNPs
    • Schenkel J. (Ed), Springer, Heidelberg, Germany
    • Kastner B. Purification and electron microscopy of spliceosomal snRNPs. In: Schenkel J. (Ed). RNP Particles, Splicing and Autoimmune Diseases (1998), Springer, Heidelberg, Germany 95-140
    • (1998) RNP Particles, Splicing and Autoimmune Diseases , pp. 95-140
    • Kastner, B.1
  • 18
    • 0025254471 scopus 로고
    • Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family
    • Kastner B., Bach M., and Lührmann R. Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family. Proc. Natl. Acad. Sci. USA 87 (1990) 1710-1714
    • (1990) Proc. Natl. Acad. Sci. USA , vol.87 , pp. 1710-1714
    • Kastner, B.1    Bach, M.2    Lührmann, R.3
  • 19
    • 0036315512 scopus 로고    scopus 로고
    • Early organization of pre-mRNA during spliceosome assembly
    • Kent O.A., and MacMillan A.M. Early organization of pre-mRNA during spliceosome assembly. Nat. Struct. Biol. 9 (2002) 576-581
    • (2002) Nat. Struct. Biol. , vol.9 , pp. 576-581
    • Kent, O.A.1    MacMillan, A.M.2
  • 20
    • 11144331491 scopus 로고    scopus 로고
    • Characterization of a U2AF-independent commitment complex (E′) in the mammalian spliceosome assembly pathway
    • Kent O.A., Ritchie D.B., and Macmillan A.M. Characterization of a U2AF-independent commitment complex (E′) in the mammalian spliceosome assembly pathway. Mol. Cell. Biol. 25 (2005) 233-240
    • (2005) Mol. Cell. Biol. , vol.25 , pp. 233-240
    • Kent, O.A.1    Ritchie, D.B.2    Macmillan, A.M.3
  • 21
    • 17844367582 scopus 로고    scopus 로고
    • The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation
    • Laggerbauer B., Liu S., Makarov E., Vornlocher H.P., Makarova O., Ingelfinger D., Achsel T., and Lührmann R. The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation. RNA 11 (2005) 598-608
    • (2005) RNA , vol.11 , pp. 598-608
    • Laggerbauer, B.1    Liu, S.2    Makarov, E.3    Vornlocher, H.P.4    Makarova, O.5    Ingelfinger, D.6    Achsel, T.7    Lührmann, R.8
  • 22
    • 0025815089 scopus 로고
    • Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts
    • Lamm G.M., Blencowe B.J., Sproat B.S., Iribarren A.M., Ryder U., and Lamond A.I. Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucleic Acids Res. 19 (1991) 3193-3198
    • (1991) Nucleic Acids Res. , vol.19 , pp. 3193-3198
    • Lamm, G.M.1    Blencowe, B.J.2    Sproat, B.S.3    Iribarren, A.M.4    Ryder, U.5    Lamond, A.I.6
  • 23
    • 0034640124 scopus 로고    scopus 로고
    • The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions
    • Makarov E.M., Makarova O.V., Achsel T., and Lührmann R. The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions. J. Mol. Biol. 298 (2000) 567-575
    • (2000) J. Mol. Biol. , vol.298 , pp. 567-575
    • Makarov, E.M.1    Makarova, O.V.2    Achsel, T.3    Lührmann, R.4
  • 24
    • 0037073946 scopus 로고    scopus 로고
    • Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome
    • Makarov E.M., Makarova O.V., Urlaub H., Gentzel M., Will C.L., Wilm M., and Lührmann R. Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome. Science 298 (2002) 2205-2208
    • (2002) Science , vol.298 , pp. 2205-2208
    • Makarov, E.M.1    Makarova, O.V.2    Urlaub, H.3    Gentzel, M.4    Will, C.L.5    Wilm, M.6    Lührmann, R.7
  • 25
    • 0035796398 scopus 로고    scopus 로고
    • Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing
    • McConnell T.S., and Steitz J.A. Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing. EMBO J. 20 (2001) 3577-3586
    • (2001) EMBO J. , vol.20 , pp. 3577-3586
    • McConnell, T.S.1    Steitz, J.A.2
  • 26
    • 0037107418 scopus 로고    scopus 로고
    • Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins
    • Nottrott S., Urlaub H., and Lührmann R. Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins. EMBO J. 21 (2002) 5527-5538
    • (2002) EMBO J. , vol.21 , pp. 5527-5538
    • Nottrott, S.1    Urlaub, H.2    Lührmann, R.3
  • 27
    • 84985231782 scopus 로고
    • Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli
    • Radermacher M., Wagenknecht T., Verschoor A., and Frank J. Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli. J. Microsc. 146 (1987) 113-136
    • (1987) J. Microsc. , vol.146 , pp. 113-136
    • Radermacher, M.1    Wagenknecht, T.2    Verschoor, A.3    Frank, J.4
  • 28
    • 0038016841 scopus 로고    scopus 로고
    • Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra
    • Sander B., Golas M.M., and Stark H. Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra. J. Struct. Biol. 142 (2003) 392-401
    • (2003) J. Struct. Biol. , vol.142 , pp. 392-401
    • Sander, B.1    Golas, M.M.2    Stark, H.3
  • 29
    • 0142187117 scopus 로고    scopus 로고
    • Corrim-based alignment for improved speed in single-particle image processing
    • Sander B., Golas M.M., and Stark H. Corrim-based alignment for improved speed in single-particle image processing. J. Struct. Biol. 143 (2003) 219-228
    • (2003) J. Struct. Biol. , vol.143 , pp. 219-228
    • Sander, B.1    Golas, M.M.2    Stark, H.3
  • 30
    • 20544469627 scopus 로고    scopus 로고
    • Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy
    • Sander B., Golas M.M., and Stark H. Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy. J. Struct. Biol. 151 (2005) 92-105
    • (2005) J. Struct. Biol. , vol.151 , pp. 92-105
    • Sander, B.1    Golas, M.M.2    Stark, H.3
  • 31
    • 0031704540 scopus 로고    scopus 로고
    • A maximum-likelihood approach to single-particle image refinement
    • Sigworth F.J. A maximum-likelihood approach to single-particle image refinement. J. Struct. Biol. 122 (1998) 328-339
    • (1998) J. Struct. Biol. , vol.122 , pp. 328-339
    • Sigworth, F.J.1
  • 32
    • 0027739853 scopus 로고
    • The U5 and U6 small nuclear RNAs as the active site components of the spliceosome
    • Sontheimer E.J., and Steitz J.A. The U5 and U6 small nuclear RNAs as the active site components of the spliceosome. Science 262 (1993) 1989-1996
    • (1993) Science , vol.262 , pp. 1989-1996
    • Sontheimer, E.J.1    Steitz, J.A.2
  • 33
    • 0032489021 scopus 로고    scopus 로고
    • Mechanical devices of the spliceosome: motors, clocks, springs, and things
    • Staley J.P., and Guthrie C. Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell 92 (1998) 315-326
    • (1998) Cell , vol.92 , pp. 315-326
    • Staley, J.P.1    Guthrie, C.2
  • 34
    • 0029031713 scopus 로고
    • Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA
    • Teigelkamp S., Newman A.J., and Beggs J.D. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA. EMBO J. 14 (1995) 2602-2612
    • (1995) EMBO J. , vol.14 , pp. 2602-2612
    • Teigelkamp, S.1    Newman, A.J.2    Beggs, J.D.3
  • 35
    • 0034731349 scopus 로고    scopus 로고
    • A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal small nuclear ribonucleoproteins (snRNPs). Analysis of RNA-protein contacts in native U1 and U4/U6.U5 snRNPs
    • Urlaub H., Hartmuth K., Kostka S., Grelle G., and Lührmann R. A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal small nuclear ribonucleoproteins (snRNPs). Analysis of RNA-protein contacts in native U1 and U4/U6.U5 snRNPs. J. Biol. Chem. 275 (2000) 41458-41468
    • (2000) J. Biol. Chem. , vol.275 , pp. 41458-41468
    • Urlaub, H.1    Hartmuth, K.2    Kostka, S.3    Grelle, G.4    Lührmann, R.5
  • 36
    • 0021645447 scopus 로고
    • Multivariate statistical classification of noisy images (randomly oriented biological macromolecules)
    • van Heel M. Multivariate statistical classification of noisy images (randomly oriented biological macromolecules). Ultramicroscopy 13 (1984) 165-183
    • (1984) Ultramicroscopy , vol.13 , pp. 165-183
    • van Heel, M.1
  • 39
    • 0025742986 scopus 로고
    • Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA
    • Wu J.A., and Manley J.L. Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA. Nature 352 (1991) 818-821
    • (1991) Nature , vol.352 , pp. 818-821
    • Wu, J.A.1    Manley, J.L.2
  • 40
    • 0027059033 scopus 로고
    • Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing
    • Wyatt J.R., Sontheimer E.J., and Steitz J.A. Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing. Genes Dev. 6 (1992) 2542-2553
    • (1992) Genes Dev. , vol.6 , pp. 2542-2553
    • Wyatt, J.R.1    Sontheimer, E.J.2    Steitz, J.A.3
  • 41
    • 0034649666 scopus 로고    scopus 로고
    • Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome
    • Yean S.L., Wuenschell G., Termini J., and Lin R.J. Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome. Nature 408 (2000) 881-884
    • (2000) Nature , vol.408 , pp. 881-884
    • Yean, S.L.1    Wuenschell, G.2    Termini, J.3    Lin, R.J.4


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