Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins

Nature Methods

Volumn 11, Issue 10, 2014, Pages 1064-1070

  Kramer, Katharina ^a   Sachsenberg, Timo ^b   Beckmann, Benedikt M ^c,e   Qamar, Saadia ^a   Boon, Kum Loong ^a   Hentze, Matthias W ^c   Kohlbacher, Oliver ^b   Urlaub, Henning ^a,d  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

^e HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSYLHOMOCYSTEINASE; ALCOHOL DEHYDROGENASE; FUNGAL RNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INORGANIC PYROPHOSPHATASE; MESSENGER RNA; NUCLEIC ACID BINDING PROTEIN; OLIGONUCLEOTIDE; PEROXIREDOXIN; PHOSPHOGLYCERATE KINASE; POLYADENYLIC ACID BINDING PROTEIN; PYRUVATE KINASE; RNA BINDING PROTEIN; AMINO ACID; CROSS LINKING REAGENT; FUNGAL PROTEIN; PEPTIDE; PROTEOME; RNA;

ARTICLE; AUTOMATION; BINDING SITE; CONTROLLED STUDY; CROSS LINKING; FUNGAL STRAIN; GEL PERMEATION CHROMATOGRAPHY; HUMAN; IN VITRO STUDY; IN VIVO STUDY; MASS SPECTROMETRY; NONHUMAN; PHOTO CROSS LINKING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CROSS LINKING; PROTEIN RNA BINDING; RNA EXTRACTION; RNA SEQUENCE; ULTRAVIOLET RADIATION; YEAST; YEAST CELL; CHEMISTRY; COMPUTER PROGRAM; COMPUTER SIMULATION; PROCEDURES; PROTEOMICS;

AMINO ACIDS; AUTOMATION; BINDING SITES; COMPUTER SIMULATION; CROSS-LINKING REAGENTS; FUNGAL PROTEINS; HUMANS; MASS SPECTROMETRY; OLIGONUCLEOTIDES; PEPTIDES; PROTEOME; PROTEOMICS; RNA; RNA-BINDING PROTEINS; SOFTWARE;

EID: 84921721617     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3092     Document Type: Article

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