An update on clostridium difficile toxinotyping

Journal of Clinical Microbiology

Volumn 54, Issue 1, 2016, Pages 13-18

  Rupnik, Maja ^a,b,c   Janezic, Sandra ^a,b  

^a National Laboratory for Health Environment and Food   (Slovenia)

^b UNIVERSITY OF MARIBOR   (Slovenia)

^c Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM TOXIN; CYTOTOXIN; VIRULENCE FACTOR; BACTERIAL PROTEIN; BACTERIAL TOXIN; ENTEROTOXIN; TCDA PROTEIN, CLOSTRIDIUM DIFFICILE; TOXB PROTEIN, CLOSTRIDIUM DIFFICILE;

BACTERIAL STRAIN; CONTROLLED STUDY; DIAGNOSTIC TEST; GENE SEQUENCE; GENETIC CODE; LABORATORY TEST; MOLECULAR DIAGNOSIS; NONHUMAN; PATHOGENICITY; PEPTOCLOSTRIDIUM DIFFICILE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVIEW; CLASSIFICATION; GENETIC VARIATION; GENETICS; GENOTYPE; HUMAN; MOLECULAR TYPING; PROCEDURES;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; ENTEROTOXINS; GENETIC VARIATION; GENOTYPE; HUMANS; MOLECULAR TYPING;

EID: 84954349270     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02083-15     Document Type: Review

References (35)

1. Pruitt RN, Lacy DB. 2012. Toward a structural understanding of Clos-tridium difficile toxins A and B. Front Cell Infect Microbiol 2:28.
2. Gerding DN, Johnson S, Rupnik M, Aktories K. 2014. Clostridium difficile binary toxin CDT: mechanism, epidemiology, and potential clinical importance. Gut Microbes 5:15-27. http://dx.doi.org/10.4161/gmic.26854.
3. Kuehne SA, Collery MM, Kelly ML, Cartman ST, Cockayne A, Minton NP. 2014. Importance of toxin A, toxin B, and CDT in virulence of an epidemic Clostridium difficile strain. J Infect Dis 209:83-86. http://dx.doi.org/10.1093/infdis/jit426.
4. Burnham C-AD, Carroll KC. 2013. Diagnosis of Clostridium difficile infection: an ongoing conundrum for clinicians and for clinical laboratories. Clin Microbiol Rev 26:604-630. http://dx.doi.org/10.1128/CMR.00016-13.
5. Rupnik M, Avesani V, Janc M, von Eichel-Streiber C, Delmée M. 1998. A novel toxinotyping scheme and correlation of toxinotypes with sero-groups of Clostridium difficile isolates. J Clin Microbiol 36:2240-2247.
6. Rupnik M. 2008. Heterogeneity of large clostridial toxins: importance of Clostridium difficile toxinotypes. FEMS Microbiol Rev 32:541-555. http://dx.doi.org/10.1111/j.1574-6976.2008.00110.x.
7. Rupnik M. 2010. Clostridium difficile toxinotyping. Methods Mol Biol 646:67-76. http://dx.doi.org/10.1007/978-1-60327-365-7-5.
8. Janezic S, Marín M, Martín A, Rupnik M. 2015. A new type of toxin A-negative, toxin B-positive Clostridium difficile strain lacking a complete tcdA gene. J Clin Microbiol 53:692-695.
9. Braun V, Hundsberger T, Leukel P, Sauerborn M, von Eichel-Streiber C. 1996. Definition of the single integration site of the pathogenicity locus in Clostridium difficile. Gene 181:29-38. http://dx.doi.org/10.1016/S0378-1119(96)00398-8.
10. Dingle KE, Elliott B, Robinson E, Griffiths D, Eyre DW, Stoesser N, Vaughan A, Golubchik T, Fawley WN, Wilcox MH, Peto TE, Walker AS, Riley TV, Crook DW, Didelot X. 2014. Evolutionary history of the Clostridium difficile pathogenicity locus. Genome Biol Evol 6:36-52. http://dx.doi.org/10.1093/gbe/evt204.
11. Elliott B, Reed R, Chang BJ, Riley TV. 2009. Bacteremia with a large clostridial toxin-negative, binary toxin-positive strain of Clostridium difficile. Anaerobe 15:249-251. http://dx.doi.org/10.1016/j.anaerobe.2009.08.006.
12. Brouwer MSM, Roberts AP, Hussain H, Williams RJ, Allan E, Mullany P. 2013. Horizontal gene transfer converts non-toxigenic Clostridium difficile strains into toxin producers. Nat Commun 4:2601. http://dx.doi.org/10.1038/ncomms3601.
13. Borriello SP, Wren BW, Hyde S, Seddon SV, Sibbons P, Krishna MM, Tabaqchali S, Manek S, Price AB. 1992. Molecular, immunological, and biological characterization ofa toxin A-negative, toxin B-positive strain of Clostridium difficile. Infect Immun 60:4192-4199.
14. Depitre C, Delmee M, Avesani V, L'Haridon R, Roels A, Popoff M, Corthier G. 1993. Serogroup F strains of Clostridium difficile produce toxin B but not toxin A. J Med Microbiol 38:434-441. http://dx.doi.org/10.1099/00222615-38-6-434.
15. Lyerly DM, Barroso LA, Wilkins TD, Depitre C, Corthier G. 1992. Characterizationof a toxin A-negative, toxin B-positive strain ofClostrid-ium difficile. Infect Immun 60:4633-4639.
16. Rupnik M, Braun V, Soehn F, Janc M, Hofstetter M, Laufenberg-Feldmann R, von Eichel-Streiber C. 1997. Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile. FEMS Microbiol Lett 148:197-202. http://dx.doi.org/10.1111/j.1574-6968.1997.tb10288.x.
17. Braun V, Mehlig M, Moos M, Rupnik M, Kalt B, Mahony DE, von Eichel-Streiber C. 2000. A chimeric ribozyme in clostridium difficile combines features of group I introns and insertion elements. Mol Micro-biol 36:1447-1459.
18. Geric B, Rupnik M, Gerding DN, Grabnar M, Johnson S. 2004. Distribution of Clostridium difficile variant toxinotypes and strains with binary toxin genes among clinical isolates in an American hospital. J Med Micro-biol 53:887-894. http://dx.doi.org/10.1099/jmm.0.45610-0.
19. Rupnik M, Brazier JS, Duerden BI, Grabnar M, Stubbs SL. 2001. Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes. Microbiology 147:439-447. http://dx.doi.org/10.1099/00221287-147-2-439.
20. Dingle KE, Griffiths D, Didelot X, Evans J, Vaughan A, Kachrimanidou M, Stoesser N, Jolley KA, Golubchik T, Harding RM, Peto TE, Fawley W, Walker AS, Wilcox M, Crook DW. 2011. Clinical Clostridium difficile: clonality and pathogenicity locus diversity. PLoS One 6:e19993. http://dx.doi.org/10.1371/journal.pone.0019993.
21. Stubbs SL, Brazier JS, O'Neill GL, Duerden BI. 1999. PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes. J Clin Microbiol 37:461-463.
22. Geric Stare B, Rupnik M. 2010. Clostridium difficile toxinotype XI (A-B-) exhibits unique arrangement of PaLoc and its upstream region. Anaerobe 16:393-395. http://dx.doi.org/10.1016/j.anaerobe.2010.04.001.
23. Rupnik M, Kato N, Grabnar M, Kato H. 2003. New types of toxin A-negative, toxin B-positive strains among Clostridium difficile isolates from Asia. J Clin Microbiol 41:1118-1125. http://dx.doi.org/10.1128/JCM.41.3.1118-1125.2003.
24. Stare BG, Delmée M, Rupnik M. 2007. Variant forms of the binary toxin CDT locus and tcdC gene in Clostridium difficile strains. J Med Microbiol 56:329-335. http://dx.doi.org/10.1099/jmm.0.46931-0.
25. Elliott B, Squire MM, Thean S, Chang BJ, Brazier JS, Rupnik M, Riley TV. 2011. New types of toxin A-negative, toxin B-positive strains among clinical isolates of Clostridium difficile in Australia. J Med Microbiol 60: 1108-1111. http://dx.doi.org/10.1099/jmm.0.031062-0.
26. Jamal WY, Rotimi VO, Grubesic A, Rupnik M, Brazier JS, Duerden BI. 2009. Correlation of multidrug resistance, toxinotypes and PCR ribotypes in Clostridium difficile isolates from Kuwait. J Chemother 21:521-526. http://dx.doi.org/10.1179/joc.2009.21.5.521.
27. Lim SK, Stuart RL, Mackin KE, Carter GP, Kotsanas D, Francis MJ, Easton M, Dimovski K, Elliott B, Riley TV, Hogg G, Paul E, Korman TM, Seemann T, Stinear TP, Lyras D, Jenkin GA. 2014. Emergence of a ribotype 244 strain of Clostridium difficile associated with severe disease and related to the epidemic ribotype 027 strain. Clin Infect Dis 58:1723-1730. http://dx.doi.org/10.1093/cid/ciu203.
28. Monot M, Eckert C, Lemire A, Hamiot A, Dubois T, Tessier C, Du-moulard B, Hamel B, Petit A, Lalande V, Ma L, Bouchier C, Barbut F, Dupuy B. 2015. Clostridium difficile: new insights into the evolution of the pathogenicity locus. Sci Rep 5:15023. http://dx.doi.org/10.1038/srep15023.
29. Rupnik M. 2001. How to detect Clostridium difficile variant strains in a routine laboratory. Clin Microbiol Infect 7:417-420. http://dx.doi.org/10.1046/j.1198-743x.2001.00290.x.
30. Lanis JM, Heinlen LD, James JA, Ballard JD. 2013. Clostridium difficile 027/BI/NAP1 encodes a hypertoxic and antigenically variable form of TcdB. PLoS Pathog 9:e1003523. http://dx.doi.org/10.1371/journal.ppat.1003523.
31. Bauer MP, Notermans DW, van Benthem BH, Brazier JS, Wilcox MH, Rupnik M, Monnet DL, van Dissel JT, Kuijper EJ. 2011. Clostridium difficile infection in Europe: a hospital-based survey. Lancet 377:63-73. http://dx.doi.org/10.1016/S0140-6736(10)61266-4.
32. Collins DA, Hawkey PM, Riley TV. 2013. Epidemiology of Clostridium difficile infection in Asia. Antimicrob Resist Infect Control 2:21. http://dx.doi.org/10.1186/2047-2994-2-21.
33. Waslawski S, Lo ES, Ewing SA, Young VB, Aronoff DM, Sharp SE, Novak-Weekley SM, Crist AE, Dunne WM, Hoppe-Bauer J, Johnson M, Brecher SM, Newton DW, Walk ST. 2013. Clostridium difficile ribotype diversity at six health care institutions in the United States. J Clin Micro-biol 51:1938-1941. http://dx.doi.org/10.1128/JCM.00056-13.
34. Eckert C, Emirian A, Le Monnier A, Cathala L, De Montclos H, Goret J, Berger P, Petit A, De Chevigny A, Jean-Pierre H, Nebbad B, Camiade S, Meckenstock R, Lalande V, Marchandin H, Barbut F. 2015. Prevalence and pathogenicity of binary toxin-positive Clostridium difficile strains that do not produce toxins A and B. New Microbes New Infect 3:12-17. http://dx.doi.org/10.1016/j.nmni.2014.10.003.
35. Janezic S, Zidaric V, Pardon B, Indra A, Kokotovic B, Blanco JL, Seyboldt C, Diaz CR, Poxton IR, Perreten V, Drigo I, Jiraskova A, Ocepek M, Weese JS, Songer JG, Wilcox MH, Rupnik M. 2014. International Clostridium difficile animal strain collection and large diversity of animal associated strains. BMC Microbiol 14:173. http://dx.doi.org/10.1186/1471-2180-14-173.